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The map label for this gene is hisH [H]

Identifier: 220915868

GI number: 220915868

Start: 843465

End: 844079

Strand: Direct

Name: hisH [H]

Synonym: A2cp1_0751

Alternate gene names: 220915868

Gene position: 843465-844079 (Clockwise)

Preceding gene: 220915867

Following gene: 220915869

Centisome position: 16.77

GC content: 76.75

Gene sequence:

>615_bases
GTGAGCCGCCCGGCCGGCACCGTCGCGCTGGTGGACTACGGCGCCGGCAACCTGCGCTCGGTGGAGAACGCGCTGCGCGA
GGTGGGCGCGGCGGTGGTGGTCACGCGCGATCCCGACGCGGTCCGCCGCGCCGACCGGGTGGTGGTGCCGGGGCAGGGCG
CGATGCCCGCCTGCGCCGAGGCGATGCGCCGGAGCGGCGTGGCCGGCGCGCTCCTGGAGGCGGTCGAGCGCGGGACGCCG
GTGCTGGGCATCTGCGTCGGGCTGCAGATCCTGTTCGCCGAGGGCGAGGAGGCGGGCGGGGCCCGCGGACTCGGCCTGGT
GGACGGCCGCATCGCGCGGCTGCCCGGCGGCGTGAAGCTGCCGCACATCGGCTGGTCGAAGGTGCGGCTGGTGGCCCCCG
GGTCGGCGCTGTTCGCGCCCATGGACGGCGCCTACGTCTACTACGCGCACTCCTACGCCGCGCCCGCGGAGGCGCCCGGC
GCGGCCCTGCTCACGCACCACGGCCGCGACTTCTGCGCGGCGCTGGAGCGCGGCAACCTGTTCGCGGTGCAGTTCCACCC
TGAGAAGAGCCAGCGCGTGGGCCTGGCCCTGCTCGAGCGGTTCGTCTCCACCTAG

Upstream 100 bases:

>100_bases
TGCACCACGTGGTCGAGGCGATCTTCAAGGCGACCGCGCGCGCGCTCCGCGCCGCGACCGCGCGCGAGGGGACCGCGCTG
CCCAGCACCAAGGGAACGCT

Downstream 100 bases:

>100_bases
GCGGGGGCCATGCTCGTCATCCCAGCGATCGATCTCATCGGCGGCGAGGTGGTCCGGCTGGAGAAGGGCGACTTCGCCCG
CAAGACCGTGTACGCGCGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSRPAGTVALVDYGAGNLRSVENALREVGAAVVVTRDPDAVRRADRVVVPGQGAMPACAEAMRRSGVAGALLEAVERGTP
VLGICVGLQILFAEGEEAGGARGLGLVDGRIARLPGGVKLPHIGWSKVRLVAPGSALFAPMDGAYVYYAHSYAAPAEAPG
AALLTHHGRDFCAALERGNLFAVQFHPEKSQRVGLALLERFVST

Sequences:

>Translated_204_residues
MSRPAGTVALVDYGAGNLRSVENALREVGAAVVVTRDPDAVRRADRVVVPGQGAMPACAEAMRRSGVAGALLEAVERGTP
VLGICVGLQILFAEGEEAGGARGLGLVDGRIARLPGGVKLPHIGWSKVRLVAPGSALFAPMDGAYVYYAHSYAAPAEAPG
AALLTHHGRDFCAALERGNLFAVQFHPEKSQRVGLALLERFVST
>Mature_203_residues
SRPAGTVALVDYGAGNLRSVENALREVGAAVVVTRDPDAVRRADRVVVPGQGAMPACAEAMRRSGVAGALLEAVERGTPV
LGICVGLQILFAEGEEAGGARGLGLVDGRIARLPGGVKLPHIGWSKVRLVAPGSALFAPMDGAYVYYAHSYAAPAEAPGA
ALLTHHGRDFCAALERGNLFAVQFHPEKSQRVGLALLERFVST

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=38.5, Blast_Score=134, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=32.8571428571429, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21206; Mature: 21074

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPAGTVALVDYGAGNLRSVENALREVGAAVVVTRDPDAVRRADRVVVPGQGAMPACAE
CCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEECCCCCCHHHHH
AMRRSGVAGALLEAVERGTPVLGICVGLQILFAEGEEAGGARGLGLVDGRIARLPGGVKL
HHHHCCHHHHHHHHHHHCCCEEEEHHCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCC
PHIGWSKVRLVAPGSALFAPMDGAYVYYAHSYAAPAEAPGAALLTHHGRDFCAALERGNL
CCCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCCCE
FAVQFHPEKSQRVGLALLERFVST
EEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRPAGTVALVDYGAGNLRSVENALREVGAAVVVTRDPDAVRRADRVVVPGQGAMPACAE
CCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEECCCCCCHHHHH
AMRRSGVAGALLEAVERGTPVLGICVGLQILFAEGEEAGGARGLGLVDGRIARLPGGVKL
HHHHCCHHHHHHHHHHHCCCEEEEHHCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCC
PHIGWSKVRLVAPGSALFAPMDGAYVYYAHSYAAPAEAPGAALLTHHGRDFCAALERGNL
CCCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCCCE
FAVQFHPEKSQRVGLALLERFVST
EEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA