Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 220915356

GI number: 220915356

Start: 249708

End: 250859

Strand: Direct

Name: proB [H]

Synonym: A2cp1_0235

Alternate gene names: 220915356

Gene position: 249708-250859 (Clockwise)

Preceding gene: 220915355

Following gene: 220915357

Centisome position: 4.97

GC content: 76.82

Gene sequence:

>1152_bases
ATGCCGCTCCCCATGTCCCGAAGCGCCCTCCGCCGCGTGAAGCGGCTCGTGGTCAAGGTCGGCACCGGCACGCTCACCGA
CCGCGCCGGCCGGTTCGATCGCGACAACTGCGCGCGGCTCGCCTCCGAGCTCGCCGAGGTGGCGCGCGGCCGGAAGGTGG
TGCTCGTCTCCTCCGGCGCGGTGGCGCTCGGCGCCGAGCGGCTCGGCCTCGCGCGCAGCCGGGGCAAGCCCTGGGACATC
CCCACCAAGCAGGCCTGCGCGGCCGCCGGCCAGCCCCACCTCATGGCCGCCTGGGGCGAGGCGCTGGGCCGCCACGGGCT
CGTCACCGCCCAGGTGCTGCTCACCGCCGACGACCTCGCCAGCCGCAAGCGCTTCCTGAACGCGCGCCGCACCTTCGAGC
GGCTGCTCGACGCGGGCGCGGTGCCGGTGGTGAACGAGAACGACACCGTGGCGGTGGACGAGATCAAGGTCGGCGACAAC
GACACGCTCGCCGCGCTGGTGGCGGGCTGCGTGGAGGCCGACGCGGTCGCGATGCTCACCGACGTGGACGGGCTCTACGA
CCGGAACCCCGCCGAGCCCGGGGCGCGGCTGCTGCGCGACGTGCCGCGGGTCACCGCCGAGATCGAGCGGAGCGCGGGCG
GGGCGGGGAGCGAGCGCAGCGTGGGCGGGATGATCACCAAGGTGAAGGCGGCGCGGCGGCTCGGTGCGCAGGGCGTCGTC
ACCGCGCTCCTCTCCGGGCGCCGCGCGCGCGCGCTCCCGGCGCTGCTCGCCGGCGAGCCGGTCGGCACCGTGTTCGCGCC
CGGGGCGCACCGGCTCAGCTCGCGCCAGGGCTGGCTCGCCGCGGCGGCGCGCGGCAAGGGCGTCATCCTGGTGGACGCGG
GGGCGCGGCGCGCGCTGGTGGAGCAGGGCCGGAGCCTGCTGCCGAGCGGCGTCCGGGGCGTGCAGGGCCAGTTCGGGGTC
GGGGACCCGGTGGACGTCGCGGTGGACCCGGCCCGCCCCTTCGCGCGGGGCCTGGCGGGCTACGGCGCCGACGAGGTCCG
CCGCATCGCCGGGCTGAAGACCGGCGAGATCGAGCGCGCGCTCGGGTATAAGTACCTCGACGAGATCGTCCACCGGAACG
ACCTGGTGGTCCTCGAGACCGGCCGGGAGTGA

Upstream 100 bases:

>100_bases
CCATCGCCGCGTCGGTGGCCTACAAGCTGCCGTGAGGCGGCGCGCCGGATCGCGCCGCCGCGCGATCCCCGCCGCGATCG
ACGCGCGGTTTGCGTCTACA

Downstream 100 bases:

>100_bases
GCATGCGCAAGGAGAAGTCGCTGGGCCTCGCCGCCGAGATGCGGAAGCTGGCGGAGGCCTCTCGCGAGGCCGCCCGGGCC
CTCTCGCACGCCGACCCCCG

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPLPMSRSALRRVKRLVVKVGTGTLTDRAGRFDRDNCARLASELAEVARGRKVVLVSSGAVALGAERLGLARSRGKPWDI
PTKQACAAAGQPHLMAAWGEALGRHGLVTAQVLLTADDLASRKRFLNARRTFERLLDAGAVPVVNENDTVAVDEIKVGDN
DTLAALVAGCVEADAVAMLTDVDGLYDRNPAEPGARLLRDVPRVTAEIERSAGGAGSERSVGGMITKVKAARRLGAQGVV
TALLSGRRARALPALLAGEPVGTVFAPGAHRLSSRQGWLAAAARGKGVILVDAGARRALVEQGRSLLPSGVRGVQGQFGV
GDPVDVAVDPARPFARGLAGYGADEVRRIAGLKTGEIERALGYKYLDEIVHRNDLVVLETGRE

Sequences:

>Translated_383_residues
MPLPMSRSALRRVKRLVVKVGTGTLTDRAGRFDRDNCARLASELAEVARGRKVVLVSSGAVALGAERLGLARSRGKPWDI
PTKQACAAAGQPHLMAAWGEALGRHGLVTAQVLLTADDLASRKRFLNARRTFERLLDAGAVPVVNENDTVAVDEIKVGDN
DTLAALVAGCVEADAVAMLTDVDGLYDRNPAEPGARLLRDVPRVTAEIERSAGGAGSERSVGGMITKVKAARRLGAQGVV
TALLSGRRARALPALLAGEPVGTVFAPGAHRLSSRQGWLAAAARGKGVILVDAGARRALVEQGRSLLPSGVRGVQGQFGV
GDPVDVAVDPARPFARGLAGYGADEVRRIAGLKTGEIERALGYKYLDEIVHRNDLVVLETGRE
>Mature_382_residues
PLPMSRSALRRVKRLVVKVGTGTLTDRAGRFDRDNCARLASELAEVARGRKVVLVSSGAVALGAERLGLARSRGKPWDIP
TKQACAAAGQPHLMAAWGEALGRHGLVTAQVLLTADDLASRKRFLNARRTFERLLDAGAVPVVNENDTVAVDEIKVGDND
TLAALVAGCVEADAVAMLTDVDGLYDRNPAEPGARLLRDVPRVTAEIERSAGGAGSERSVGGMITKVKAARRLGAQGVVT
ALLSGRRARALPALLAGEPVGTVFAPGAHRLSSRQGWLAAAARGKGVILVDAGARRALVEQGRSLLPSGVRGVQGQFGVG
DPVDVAVDPARPFARGLAGYGADEVRRIAGLKTGEIERALGYKYLDEIVHRNDLVVLETGRE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=297, Percent_Identity=32.3232323232323, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI62912457, Length=295, Percent_Identity=32.2033898305085, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=40.2739726027397, Blast_Score=238, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17569967, Length=285, Percent_Identity=35.4385964912281, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569969, Length=285, Percent_Identity=35.4385964912281, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=32.3741007194245, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=30, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21357643, Length=299, Percent_Identity=33.7792642140468, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40264; Mature: 40133

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPMSRSALRRVKRLVVKVGTGTLTDRAGRFDRDNCARLASELAEVARGRKVVLVSSGA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCC
VALGAERLGLARSRGKPWDIPTKQACAAAGQPHLMAAWGEALGRHGLVTAQVLLTADDLA
CEECHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
SRKRFLNARRTFERLLDAGAVPVVNENDTVAVDEIKVGDNDTLAALVAGCVEADAVAMLT
HHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
DVDGLYDRNPAEPGARLLRDVPRVTAEIERSAGGAGSERSVGGMITKVKAARRLGAQGVV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHH
TALLSGRRARALPALLAGEPVGTVFAPGAHRLSSRQGWLAAAARGKGVILVDAGARRALV
HHHHCCCHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCEEECCCCCEEEEECCCCHHHHH
EQGRSLLPSGVRGVQGQFGVGDPVDVAVDPARPFARGLAGYGADEVRRIAGLKTGEIERA
HCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHH
LGYKYLDEIVHRNDLVVLETGRE
HHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
PLPMSRSALRRVKRLVVKVGTGTLTDRAGRFDRDNCARLASELAEVARGRKVVLVSSGA
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCC
VALGAERLGLARSRGKPWDIPTKQACAAAGQPHLMAAWGEALGRHGLVTAQVLLTADDLA
CEECHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
SRKRFLNARRTFERLLDAGAVPVVNENDTVAVDEIKVGDNDTLAALVAGCVEADAVAMLT
HHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
DVDGLYDRNPAEPGARLLRDVPRVTAEIERSAGGAGSERSVGGMITKVKAARRLGAQGVV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHH
TALLSGRRARALPALLAGEPVGTVFAPGAHRLSSRQGWLAAAARGKGVILVDAGARRALV
HHHHCCCHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCEEECCCCCEEEEECCCCHHHHH
EQGRSLLPSGVRGVQGQFGVGDPVDVAVDPARPFARGLAGYGADEVRRIAGLKTGEIERA
HCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHH
LGYKYLDEIVHRNDLVVLETGRE
HHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA