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The map label for this gene is thiF [C]

Identifier: 220905430

GI number: 220905430

Start: 2612585

End: 2613229

Strand: Direct

Name: thiF [C]

Synonym: Ddes_2168

Alternate gene names: 220905430

Gene position: 2612585-2613229 (Clockwise)

Preceding gene: 220905429

Following gene: 220905440

Centisome position: 90.92

GC content: 63.1

Gene sequence:

>645_bases
ATGAAAGAAAGAACATCCGTAACAAATCCCCTGTGCCAGGGGCTTGCCCGCTATATGGCCCCGGAGCAGCTGGCAATCTT
GCAGCAGGCCCGCATTGGCATCGCCGGTGCGGGCGGTCTCGGTTCCAATGCGGCCCTTATGCTGACCCGCAGCGGCGTGG
AGCACCTGACTCTGGTGGATGATGACGTGGTGGACGCTTCCAACCTGAACCGGCAGCAGTACTGGCCGCGTCATGTGGGC
CGCTGCAAGGTGGACGCCCTGCATGAAATATTGCTGGAGCTGAACCCCCGGATGATGGTGGACTCCCGCAAAATGCGCAT
TACCAGCGCCAGCCTGCCGGGCCTGCTGCCTCTCTGCCCCATATGGGTGGAGGCCCTGGACAATCCTGAAGACAAAAGCC
TTTTTGTTGAAAGCGCCCTGCTGGCTGGGCACCGGGTCGCCAGCGCCTCCGGCATGGGGGGCTTCGGCGGTGCTCCCATG
GGGCGACGCAGGCTCGGCAACCTCGTGCTGGTGGGTGACTTTACTACAGATATTCTCATGGCTCCGCCGCTCGCACCCCG
CGTAACAGAGGCGGCGGCCCTGCTGGCCGATGCCGTGCTGGAAATGCTGCTCGGCGGTCCACATATGCCGGAAACCGCGC
TTTGA

Upstream 100 bases:

>100_bases
GACGGCCGCAGCCTGGAGCAGATGGTCTCGGCCCTCGCAGGCATGGGTTACCAGCCCGTGCTCAAGGATTGGGAAGACCC
CGTGGGCGCATGGGCATGAC

Downstream 100 bases:

>100_bases
ACCGGAACAAAAGACCATAGAAACAGGGCCCATCAGATCCAAAACTCCGGCTGCCGCATTCCGGCAGCCGGAGTACAGAT
CCTTCCCCACCCCTTGCCAC

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Molybdopterin Biosynthesis Protein MoeB; LOW QUALITY PROTEIN Thiamine Biosynthesis Protein ThiF; Thiazole Biosynthesis Protein ThiF

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKERTSVTNPLCQGLARYMAPEQLAILQQARIGIAGAGGLGSNAALMLTRSGVEHLTLVDDDVVDASNLNRQQYWPRHVG
RCKVDALHEILLELNPRMMVDSRKMRITSASLPGLLPLCPIWVEALDNPEDKSLFVESALLAGHRVASASGMGGFGGAPM
GRRRLGNLVLVGDFTTDILMAPPLAPRVTEAAALLADAVLEMLLGGPHMPETAL

Sequences:

>Translated_214_residues
MKERTSVTNPLCQGLARYMAPEQLAILQQARIGIAGAGGLGSNAALMLTRSGVEHLTLVDDDVVDASNLNRQQYWPRHVG
RCKVDALHEILLELNPRMMVDSRKMRITSASLPGLLPLCPIWVEALDNPEDKSLFVESALLAGHRVASASGMGGFGGAPM
GRRRLGNLVLVGDFTTDILMAPPLAPRVTEAAALLADAVLEMLLGGPHMPETAL
>Mature_214_residues
MKERTSVTNPLCQGLARYMAPEQLAILQQARIGIAGAGGLGSNAALMLTRSGVEHLTLVDDDVVDASNLNRQQYWPRHVG
RCKVDALHEILLELNPRMMVDSRKMRITSASLPGLLPLCPIWVEALDNPEDKSLFVESALLAGHRVASASGMGGFGGAPM
GRRRLGNLVLVGDFTTDILMAPPLAPRVTEAAALLADAVLEMLLGGPHMPETAL

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6321903, Length=131, Percent_Identity=32.0610687022901, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 22865; Mature: 22865

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERTSVTNPLCQGLARYMAPEQLAILQQARIGIAGAGGLGSNAALMLTRSGVEHLTLVD
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCCEEEECC
DDVVDASNLNRQQYWPRHVGRCKVDALHEILLELNPRMMVDSRKMRITSASLPGLLPLCP
CCCCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCHHHHH
IWVEALDNPEDKSLFVESALLAGHRVASASGMGGFGGAPMGRRRLGNLVLVGDFTTDILM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEEECCCHHHHC
APPLAPRVTEAAALLADAVLEMLLGGPHMPETAL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKERTSVTNPLCQGLARYMAPEQLAILQQARIGIAGAGGLGSNAALMLTRSGVEHLTLVD
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCCEEEECC
DDVVDASNLNRQQYWPRHVGRCKVDALHEILLELNPRMMVDSRKMRITSASLPGLLPLCP
CCCCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCHHHHH
IWVEALDNPEDKSLFVESALLAGHRVASASGMGGFGGAPMGRRRLGNLVLVGDFTTDILM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEEECCCHHHHC
APPLAPRVTEAAALLADAVLEMLLGGPHMPETAL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA