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The map label for this gene is thiH [H]

Identifier: 220905429

GI number: 220905429

Start: 2611459

End: 2612583

Strand: Direct

Name: thiH [H]

Synonym: Ddes_2167

Alternate gene names: 220905429

Gene position: 2611459-2612583 (Clockwise)

Preceding gene: 220905428

Following gene: 220905430

Centisome position: 90.88

GC content: 62.13

Gene sequence:

>1125_bases
ATGGAAACTTTTCAGGACATCTTGCAGGCATGGCCCGCGCAACGCCGGACTGATGCGGTACAGGCGGCTACAGAGGAACA
CGTCTTTTCCGCCCTGCAGAAAAACATCCTTCAGCCCGAGGACTTCCTGGCCCTGCTCTCCCCTGCGGCCAGCCCACATC
TGGAGCTGATGGCCCGGCGCGCCCACGAGCTGACCCTGCGCTTTTTCGGCCGGGCGGTGCATATCTTCACGCCGCTCTAC
ATTTCGGACGTCTGCACAAACCAGTGCCGCTACTGTGGCTTCAACGCCAAGAACAAGCAGGCCCGCCGCCACCTCAGCGT
GGATGAAGCCGCTGCCGAGGCCAAAGCCGTGGCTGATCTCGGCTTTCAGCATATCCTGCTGCTTACCGGCGACGCGCGGC
ACCTGTCTTCGCCGGAATATATCGCCGCTGTGGCCCGGCGTATCAAGCCCTGGTTCGCCTCTGTAGGCATCGAGGTGTAC
TCTCTCACCACCGAAGAGTATGCCATGCTTGTGGATGCGGGCGTGGACAGCATGACCATGTTTCAGGAAACCTATGCTCC
TGAGCTGTATGGATGGCTGCACCCTGCCGGTCCCAAACGCGACTACGCCTTTCGCCTCAACGCGCCCCAGCGCGCCGCAC
AGGCCGGCATGCGATCAATGGGTGTTGGTGCGCTGCTGGGCCTGGACTCTTTCGAACAGGACGCTTTTGCCACCGGCTTG
CACGCATGGTGGCTGCAACGGCGCTACCCAGGCATGGACGTAAGCGTGTCCATACCGCGCATCTGCCCCCATGAGGGCAG
CTTTGACGTGCCGCATGCCCTGGATGACCGCCACCTCGTACAATACGTAACAGCGCTGCGCTGTTTTCTGCCAAGGGCTG
GCATCACCTGCTCCAGCCGCGAAAGCGCCTTTATGCGTGATCACCTGCTGCCGCTGGGTGTAACGCGGGTATCTGCGGGC
GTGTCCACTGCCGTGGGTGGGCGTGTCACAGAAGACGCCCATAATCCCGGCCAGTTTGAAATCACCGACGGCCGCAGCCT
GGAGCAGATGGTCTCGGCCCTCGCAGGCATGGGTTACCAGCCCGTGCTCAAGGATTGGGAAGACCCCGTGGGCGCATGGG
CATGA

Upstream 100 bases:

>100_bases
CGCAACGCATGGCTTGCAGGGCTTGGAAATGTGCGCGGCATGGCCGCCGGAGCCGACGCCTCATCCCCTCTTACGGGCTT
TTTGCGCTGAGGAGGCAATG

Downstream 100 bases:

>100_bases
CATGAAAGAAAGAACATCCGTAACAAATCCCCTGTGCCAGGGGCTTGCCCGCTATATGGCCCCGGAGCAGCTGGCAATCT
TGCAGCAGGCCCGCATTGGC

Product: thiazole biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
METFQDILQAWPAQRRTDAVQAATEEHVFSALQKNILQPEDFLALLSPAASPHLELMARRAHELTLRFFGRAVHIFTPLY
ISDVCTNQCRYCGFNAKNKQARRHLSVDEAAAEAKAVADLGFQHILLLTGDARHLSSPEYIAAVARRIKPWFASVGIEVY
SLTTEEYAMLVDAGVDSMTMFQETYAPELYGWLHPAGPKRDYAFRLNAPQRAAQAGMRSMGVGALLGLDSFEQDAFATGL
HAWWLQRRYPGMDVSVSIPRICPHEGSFDVPHALDDRHLVQYVTALRCFLPRAGITCSSRESAFMRDHLLPLGVTRVSAG
VSTAVGGRVTEDAHNPGQFEITDGRSLEQMVSALAGMGYQPVLKDWEDPVGAWA

Sequences:

>Translated_374_residues
METFQDILQAWPAQRRTDAVQAATEEHVFSALQKNILQPEDFLALLSPAASPHLELMARRAHELTLRFFGRAVHIFTPLY
ISDVCTNQCRYCGFNAKNKQARRHLSVDEAAAEAKAVADLGFQHILLLTGDARHLSSPEYIAAVARRIKPWFASVGIEVY
SLTTEEYAMLVDAGVDSMTMFQETYAPELYGWLHPAGPKRDYAFRLNAPQRAAQAGMRSMGVGALLGLDSFEQDAFATGL
HAWWLQRRYPGMDVSVSIPRICPHEGSFDVPHALDDRHLVQYVTALRCFLPRAGITCSSRESAFMRDHLLPLGVTRVSAG
VSTAVGGRVTEDAHNPGQFEITDGRSLEQMVSALAGMGYQPVLKDWEDPVGAWA
>Mature_374_residues
METFQDILQAWPAQRRTDAVQAATEEHVFSALQKNILQPEDFLALLSPAASPHLELMARRAHELTLRFFGRAVHIFTPLY
ISDVCTNQCRYCGFNAKNKQARRHLSVDEAAAEAKAVADLGFQHILLLTGDARHLSSPEYIAAVARRIKPWFASVGIEVY
SLTTEEYAMLVDAGVDSMTMFQETYAPELYGWLHPAGPKRDYAFRLNAPQRAAQAGMRSMGVGALLGLDSFEQDAFATGL
HAWWLQRRYPGMDVSVSIPRICPHEGSFDVPHALDDRHLVQYVTALRCFLPRAGITCSSRESAFMRDHLLPLGVTRVSAG
VSTAVGGRVTEDAHNPGQFEITDGRSLEQMVSALAGMGYQPVLKDWEDPVGAWA

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=375, Percent_Identity=37.3333333333333, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41445; Mature: 41445

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFQDILQAWPAQRRTDAVQAATEEHVFSALQKNILQPEDFLALLSPAASPHLELMARR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHH
AHELTLRFFGRAVHIFTPLYISDVCTNQCRYCGFNAKNKQARRHLSVDEAAAEAKAVADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHC
GFQHILLLTGDARHLSSPEYIAAVARRIKPWFASVGIEVYSLTTEEYAMLVDAGVDSMTM
CCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCHHHH
FQETYAPELYGWLHPAGPKRDYAFRLNAPQRAAQAGMRSMGVGALLGLDSFEQDAFATGL
HHHHHCHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHH
HAWWLQRRYPGMDVSVSIPRICPHEGSFDVPHALDDRHLVQYVTALRCFLPRAGITCSSR
HHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
ESAFMRDHLLPLGVTRVSAGVSTAVGGRVTEDAHNPGQFEITDGRSLEQMVSALAGMGYQ
HHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCC
PVLKDWEDPVGAWA
HHHHCCCCCCCCCC
>Mature Secondary Structure
METFQDILQAWPAQRRTDAVQAATEEHVFSALQKNILQPEDFLALLSPAASPHLELMARR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHH
AHELTLRFFGRAVHIFTPLYISDVCTNQCRYCGFNAKNKQARRHLSVDEAAAEAKAVADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHC
GFQHILLLTGDARHLSSPEYIAAVARRIKPWFASVGIEVYSLTTEEYAMLVDAGVDSMTM
CCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCHHHH
FQETYAPELYGWLHPAGPKRDYAFRLNAPQRAAQAGMRSMGVGALLGLDSFEQDAFATGL
HHHHHCHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHH
HAWWLQRRYPGMDVSVSIPRICPHEGSFDVPHALDDRHLVQYVTALRCFLPRAGITCSSR
HHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
ESAFMRDHLLPLGVTRVSAGVSTAVGGRVTEDAHNPGQFEITDGRSLEQMVSALAGMGYQ
HHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCC
PVLKDWEDPVGAWA
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]