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The map label for this gene is tgt [H]

Identifier: 220905288

GI number: 220905288

Start: 2438030

End: 2439253

Strand: Direct

Name: tgt [H]

Synonym: Ddes_2026

Alternate gene names: 220905288

Gene position: 2438030-2439253 (Clockwise)

Preceding gene: 220905274

Following gene: 220905289

Centisome position: 84.85

GC content: 61.36

Gene sequence:

>1224_bases
ATGTCCGGCGGGCGTCATGCATTGCAGGCCGGGCACACGCAGGACTGCTTGCATCCCGGCCCGCTTGCATATACTGTGCG
CGGCATGTCCGATACCATTTTTACCATAGAGCATACCGACGGGGCCGCCCGCGCAGGAATCCTGCGCACGGCACACGGCA
CGATTCCCACACCTATCTTCATGCCCGTGGGCACAGTAGGCTCGGTCAAGGCCATTGCTCCTGACGACCTGGCCGCCATC
GGCGCGCCCGTGATACTCGGCAATACCTACCATCTGTACCTGCGCCCCGGCGACGAACTGGTGGCCCGGCGCGGCGGCCT
GCACAAGTTCGCCTCCTGGCCCGGCTCCATTCTTACCGACAGCGGCGGTTTTCAGGTCTTCAGCCTCAGCTCGCTGCGCA
AAATCCGTGAAGAAGGGGTGGAGTTCCGCTCGCACCTTGACGGTTCCCGTCATCTGTTCACGCCTGAAAAGGTGTTGCAG
ATACAGCGCAACCTCAACTCCGACATCATGATGGTGCTTGATGAGTGCGTGCCGTATGGCGCGGACTATGACTATACCGA
AAAATCACTGGCTCTCACCACACGGTGGGCACTGCGCGCCCGGGCGGCCTACCCGGCAGGCACGGCGCACAACCTGCTTT
TTGCCATCACTCAGGGCGGCTTTCATAAAGACCTGCGCACGCGCTCGGTGGAAGAGCTGTGCGGCCTTGACTTTGACGGC
TTTGCCATCGGCGGCCTTTCGGTGGGCGAACCCAAAGACCAGATGTATGACCTGCTCTATCACACCGCCCCCCAGCTGCC
CGGCAACAAACCCCGCTATCTTATGGGCGTGGGTACGCCGCTTGATATCGCCAACGGCATCAACGCCGGGGTGGACATGT
TCGACTGCGTGCTGCCCACCCGCAACGCGCGCAACGGCACGCTCTATACCTCACAGGGAAAAATAAACATCAGGCGCAGG
CAGTTTGCCGAAGACGACGGCCCCCTGGACCCCAACTGCCGCTGCTACACCTGCCGCAACTTTTCGCGCGCCTACCTGCG
CCATCTGTATGCCAGCCAGGAGCTGCTTTCTTTTCGCCTCAACTCGCTGCACAATCTCACGTACTTTCTCGATCTGGCCC
GCAATGCGCGCCAGGCCATCATTGAAGGCCGATACGCCGCCTTTCTTGCAGGCATTGAGGCTCTCTATCCCGATGAGGCG
GCAAGAGCCGCTGCCGGAGCATAG

Upstream 100 bases:

>100_bases
CCCCTTTTTCGTGTCTGGAAGCGCAAGACAGAACAGATAGCTCTACCGATAATAGATTCATTTATTCCGGCATACGATCT
TTGTCAAACATCCATCCGGC

Downstream 100 bases:

>100_bases
AGCAGTTTCAAAGTGAAAATGCTTTGGCGTATGCTCGCGCAGACGTCCGCCGTGGAGGCACAGGCACAATGTAGTTACGC
TGTTACGCGCAGAGCGAACG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSGGRHALQAGHTQDCLHPGPLAYTVRGMSDTIFTIEHTDGAARAGILRTAHGTIPTPIFMPVGTVGSVKAIAPDDLAAI
GAPVILGNTYHLYLRPGDELVARRGGLHKFASWPGSILTDSGGFQVFSLSSLRKIREEGVEFRSHLDGSRHLFTPEKVLQ
IQRNLNSDIMMVLDECVPYGADYDYTEKSLALTTRWALRARAAYPAGTAHNLLFAITQGGFHKDLRTRSVEELCGLDFDG
FAIGGLSVGEPKDQMYDLLYHTAPQLPGNKPRYLMGVGTPLDIANGINAGVDMFDCVLPTRNARNGTLYTSQGKINIRRR
QFAEDDGPLDPNCRCYTCRNFSRAYLRHLYASQELLSFRLNSLHNLTYFLDLARNARQAIIEGRYAAFLAGIEALYPDEA
ARAAAGA

Sequences:

>Translated_407_residues
MSGGRHALQAGHTQDCLHPGPLAYTVRGMSDTIFTIEHTDGAARAGILRTAHGTIPTPIFMPVGTVGSVKAIAPDDLAAI
GAPVILGNTYHLYLRPGDELVARRGGLHKFASWPGSILTDSGGFQVFSLSSLRKIREEGVEFRSHLDGSRHLFTPEKVLQ
IQRNLNSDIMMVLDECVPYGADYDYTEKSLALTTRWALRARAAYPAGTAHNLLFAITQGGFHKDLRTRSVEELCGLDFDG
FAIGGLSVGEPKDQMYDLLYHTAPQLPGNKPRYLMGVGTPLDIANGINAGVDMFDCVLPTRNARNGTLYTSQGKINIRRR
QFAEDDGPLDPNCRCYTCRNFSRAYLRHLYASQELLSFRLNSLHNLTYFLDLARNARQAIIEGRYAAFLAGIEALYPDEA
ARAAAGA
>Mature_406_residues
SGGRHALQAGHTQDCLHPGPLAYTVRGMSDTIFTIEHTDGAARAGILRTAHGTIPTPIFMPVGTVGSVKAIAPDDLAAIG
APVILGNTYHLYLRPGDELVARRGGLHKFASWPGSILTDSGGFQVFSLSSLRKIREEGVEFRSHLDGSRHLFTPEKVLQI
QRNLNSDIMMVLDECVPYGADYDYTEKSLALTTRWALRARAAYPAGTAHNLLFAITQGGFHKDLRTRSVEELCGLDFDGF
AIGGLSVGEPKDQMYDLLYHTAPQLPGNKPRYLMGVGTPLDIANGINAGVDMFDCVLPTRNARNGTLYTSQGKINIRRRQ
FAEDDGPLDPNCRCYTCRNFSRAYLRHLYASQELLSFRLNSLHNLTYFLDLARNARQAIIEGRYAAFLAGIEALYPDEAA
RAAAGA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=45.4545454545455, Blast_Score=318, Evalue=8e-87,
Organism=Escherichia coli, GI1786607, Length=357, Percent_Identity=52.1008403361345, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17544680, Length=350, Percent_Identity=41.7142857142857, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25146502, Length=182, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920510, Length=377, Percent_Identity=42.9708222811671, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI21355311, Length=192, Percent_Identity=29.1666666666667, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44588; Mature: 44457

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGRHALQAGHTQDCLHPGPLAYTVRGMSDTIFTIEHTDGAARAGILRTAHGTIPTPIF
CCCCCCHHHCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHCEEEECCCCCCCCCE
MPVGTVGSVKAIAPDDLAAIGAPVILGNTYHLYLRPGDELVARRGGLHKFASWPGSILTD
EECCCCCCEEEECCCHHHHHCCCEEECCEEEEEECCCHHHHHHCCCCHHHHCCCCCEEEC
SGGFQVFSLSSLRKIREEGVEFRSHLDGSRHLFTPEKVLQIQRNLNSDIMMVLDECVPYG
CCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
ADYDYTEKSLALTTRWALRARAAYPAGTAHNLLFAITQGGFHKDLRTRSVEELCGLDFDG
CCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC
FAIGGLSVGEPKDQMYDLLYHTAPQLPGNKPRYLMGVGTPLDIANGINAGVDMFDCVLPT
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCCCHHHHHHCCC
RNARNGTLYTSQGKINIRRRQFAEDDGPLDPNCRCYTCRNFSRAYLRHLYASQELLSFRL
CCCCCCEEEECCCCEEEHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
NSLHNLTYFLDLARNARQAIIEGRYAAFLAGIEALYPDEAARAAAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SGGRHALQAGHTQDCLHPGPLAYTVRGMSDTIFTIEHTDGAARAGILRTAHGTIPTPIF
CCCCCHHHCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHCEEEECCCCCCCCCE
MPVGTVGSVKAIAPDDLAAIGAPVILGNTYHLYLRPGDELVARRGGLHKFASWPGSILTD
EECCCCCCEEEECCCHHHHHCCCEEECCEEEEEECCCHHHHHHCCCCHHHHCCCCCEEEC
SGGFQVFSLSSLRKIREEGVEFRSHLDGSRHLFTPEKVLQIQRNLNSDIMMVLDECVPYG
CCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
ADYDYTEKSLALTTRWALRARAAYPAGTAHNLLFAITQGGFHKDLRTRSVEELCGLDFDG
CCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC
FAIGGLSVGEPKDQMYDLLYHTAPQLPGNKPRYLMGVGTPLDIANGINAGVDMFDCVLPT
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCCCHHHHHHCCC
RNARNGTLYTSQGKINIRRRQFAEDDGPLDPNCRCYTCRNFSRAYLRHLYASQELLSFRL
CCCCCCEEEECCCCEEEHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
NSLHNLTYFLDLARNARQAIIEGRYAAFLAGIEALYPDEAARAAAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA