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The map label for this gene is ispE [H]

Identifier: 220904718

GI number: 220904718

Start: 1738034

End: 1738921

Strand: Direct

Name: ispE [H]

Synonym: Ddes_1450

Alternate gene names: 220904718

Gene position: 1738034-1738921 (Clockwise)

Preceding gene: 220904717

Following gene: 220904719

Centisome position: 60.49

GC content: 58.45

Gene sequence:

>888_bases
ATGAGCATTCTCACCGCCGGTTGCAAGGTAAATCTGGGCTTGCGCATCACCGGGGTTCGCCCCGACGGCTATCACGAAAT
CGACTCTCTGTTCTACCCCTTGCCACGCCCCTGTGATCAGATAAAGCTGCGCGTCACTGACGTCAGTGGTATTTCCGTGC
TCTGCACAGGGCCGGAGGCCGGGCTGCTGGATGCGGAAAACAATACCCTTACCAGGGCCTACACAGCCTTTGCCCAGGCA
GTGGATTTCCCTGTTCCCGGCCTTGAAGCGCGTTTGCGCAAGGGTATTCCTTCCGGGGCCGGTCTTGGCGGCGGCAGCAG
CGATGCGGCAGCCCTGCTGCGCTGGCTTAACGGGCATGTTGCCACACCGCTCTCGTCACAGTCTCTGGCAGAGGTAGCCC
TTGGTGTGGGGGCCGATGTGCCTTTTTTTTTGCACAATTGCCCCTGCCGTGTGCGCGGCATAGGGGAAATTATCGAATCT
GTTGACCTGGATGTTTCAGGCATGCGTCTGGTGCTCGTATGCCCGGAAATCCATGTAAGCACGCCACAAACCTTTGCTGA
TTACGATGCTTTTGCGGCTGCCTCCCACGCTGCCGAAAAGCAAAACAGCTTGACAAGAGAGCAAAGCAGGGCTAATGGAA
CTTTTCTCTCCCAAGGGCGGTTTGACGCTGACTTGGGCAACGATCTAGAAAGCGTTGTCTTCAGACGTCATCCCGAGCTG
GCCGACATCAAGGCCACCCTGCTGCGCTACGGGGCAACAGCGGCTTGCATGAGCGGCAGCGGCTCCAGCATACTGGGGCT
TTTCAGGCGCGAAGCGCTTGTGGAAGCACAGGCTGCCGTTGCCATGCTGCAAGGAGAGAACAGGCGCGTTTACGCGCATG
TGCTGTGA

Upstream 100 bases:

>100_bases
CCCGCAGCATCCACGCGCTCCGCAAACGGCGTCACACATACGGCTTAGAGAACATACGAGATAAAAAACATCAGACCAGG
GATATGGCAAGGATACGTTC

Downstream 100 bases:

>100_bases
TTATACTGGGATGTAGCCAAGTGGTAAGGCAACGGGTTTTGGCTCCGTCATTCGAAGGTTCGAACCCTTCCATCCCAGCC
ACACACCATGCACGACGCGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSILTAGCKVNLGLRITGVRPDGYHEIDSLFYPLPRPCDQIKLRVTDVSGISVLCTGPEAGLLDAENNTLTRAYTAFAQA
VDFPVPGLEARLRKGIPSGAGLGGGSSDAAALLRWLNGHVATPLSSQSLAEVALGVGADVPFFLHNCPCRVRGIGEIIES
VDLDVSGMRLVLVCPEIHVSTPQTFADYDAFAAASHAAEKQNSLTREQSRANGTFLSQGRFDADLGNDLESVVFRRHPEL
ADIKATLLRYGATAACMSGSGSSILGLFRREALVEAQAAVAMLQGENRRVYAHVL

Sequences:

>Translated_295_residues
MSILTAGCKVNLGLRITGVRPDGYHEIDSLFYPLPRPCDQIKLRVTDVSGISVLCTGPEAGLLDAENNTLTRAYTAFAQA
VDFPVPGLEARLRKGIPSGAGLGGGSSDAAALLRWLNGHVATPLSSQSLAEVALGVGADVPFFLHNCPCRVRGIGEIIES
VDLDVSGMRLVLVCPEIHVSTPQTFADYDAFAAASHAAEKQNSLTREQSRANGTFLSQGRFDADLGNDLESVVFRRHPEL
ADIKATLLRYGATAACMSGSGSSILGLFRREALVEAQAAVAMLQGENRRVYAHVL
>Mature_294_residues
SILTAGCKVNLGLRITGVRPDGYHEIDSLFYPLPRPCDQIKLRVTDVSGISVLCTGPEAGLLDAENNTLTRAYTAFAQAV
DFPVPGLEARLRKGIPSGAGLGGGSSDAAALLRWLNGHVATPLSSQSLAEVALGVGADVPFFLHNCPCRVRGIGEIIESV
DLDVSGMRLVLVCPEIHVSTPQTFADYDAFAAASHAAEKQNSLTREQSRANGTFLSQGRFDADLGNDLESVVFRRHPELA
DIKATLLRYGATAACMSGSGSSILGLFRREALVEAQAAVAMLQGENRRVYAHVL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=270, Percent_Identity=31.4814814814815, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31334; Mature: 31203

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILTAGCKVNLGLRITGVRPDGYHEIDSLFYPLPRPCDQIKLRVTDVSGISVLCTGPEA
CCEEECCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCC
GLLDAENNTLTRAYTAFAQAVDFPVPGLEARLRKGIPSGAGLGGGSSDAAALLRWLNGHV
CEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
ATPLSSQSLAEVALGVGADVPFFLHNCPCRVRGIGEIIESVDLDVSGMRLVLVCPEIHVS
CCCCCHHHHHHHHHHCCCCCCHHHHCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCCC
TPQTFADYDAFAAASHAAEKQNSLTREQSRANGTFLSQGRFDADLGNDLESVVFRRHPEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCH
ADIKATLLRYGATAACMSGSGSSILGLFRREALVEAQAAVAMLQGENRRVYAHVL
HHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
SILTAGCKVNLGLRITGVRPDGYHEIDSLFYPLPRPCDQIKLRVTDVSGISVLCTGPEA
CEEECCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCC
GLLDAENNTLTRAYTAFAQAVDFPVPGLEARLRKGIPSGAGLGGGSSDAAALLRWLNGHV
CEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
ATPLSSQSLAEVALGVGADVPFFLHNCPCRVRGIGEIIESVDLDVSGMRLVLVCPEIHVS
CCCCCHHHHHHHHHHCCCCCCHHHHCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCCC
TPQTFADYDAFAAASHAAEKQNSLTREQSRANGTFLSQGRFDADLGNDLESVVFRRHPEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCH
ADIKATLLRYGATAACMSGSGSSILGLFRREALVEAQAAVAMLQGENRRVYAHVL
HHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA