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The map label for this gene is hslV

Identifier: 220904717

GI number: 220904717

Start: 1737240

End: 1737773

Strand: Direct

Name: hslV

Synonym: Ddes_1449

Alternate gene names: 220904717

Gene position: 1737240-1737773 (Clockwise)

Preceding gene: 220904716

Following gene: 220904718

Centisome position: 60.46

GC content: 58.8

Gene sequence:

>534_bases
ATGGAAACACATGCCACTACAATTCTCGCCGTTCGTAAGGACGGTATCGTGGCCCTGGCCGGTGACGGGCAGGTGACCAT
GGGGCAGACCATGATCATGAAGCATGCGGCCCAGAAAGTACGCCGCCTGCATGACGGCAAGATTCTGGCCGGTTTTGCCG
GAGCCACCGCCGATGCGTTTACCCTTTTTGAACTTTTTGAATCCAAGCTCAAGGAAGTACGCGGCCATATGGTCCGGGCC
GCCGTGGAAATGACCAAGGACTGGCGCAAGGACAAATACCTGCGCAAGCTGGAGGCCATGCTGCTGCTGGCCGACAGGGA
ACATATCCTGGTGCTTTCAGGCACAGGCGATGTTATCGAACCGGACGACAATGTGGCTGCCATCGGCAGCGGCGGCCCCT
ATGCCCTGGCGGCCGCGCGGGCGCTTTCCCGCCACAGCGGGCTTGACGCTGAAACCATCGCCAGGGAATCCATGCGCATT
GCCGCCGAAATATGCGTTTACACCAATGATCACGTAACCCTTGAAACCCTGTAG

Upstream 100 bases:

>100_bases
ACCAGGCCAAGAGCATGGCCTCCCGCACGACGGACAAGCGCGCTTTTCAGCGCCTTGAGACCGCCTACAAAGAACGCAAG
GAAATATGGGACAAGAACTG

Downstream 100 bases:

>100_bases
GACGGCCTGACGCCACATGGCGCCGCTGCCCTGCGCCGGGGAACTCGACGGGGATTCCAAGGCTATATTGCACTGAAAGC
GCAACGTGACGCAATATCCG

Product: ATP-dependent protease peptidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
METHATTILAVRKDGIVALAGDGQVTMGQTMIMKHAAQKVRRLHDGKILAGFAGATADAFTLFELFESKLKEVRGHMVRA
AVEMTKDWRKDKYLRKLEAMLLLADREHILVLSGTGDVIEPDDNVAAIGSGGPYALAAARALSRHSGLDAETIARESMRI
AAEICVYTNDHVTLETL

Sequences:

>Translated_177_residues
METHATTILAVRKDGIVALAGDGQVTMGQTMIMKHAAQKVRRLHDGKILAGFAGATADAFTLFELFESKLKEVRGHMVRA
AVEMTKDWRKDKYLRKLEAMLLLADREHILVLSGTGDVIEPDDNVAAIGSGGPYALAAARALSRHSGLDAETIARESMRI
AAEICVYTNDHVTLETL
>Mature_177_residues
METHATTILAVRKDGIVALAGDGQVTMGQTMIMKHAAQKVRRLHDGKILAGFAGATADAFTLFELFESKLKEVRGHMVRA
AVEMTKDWRKDKYLRKLEAMLLLADREHILVLSGTGDVIEPDDNVAAIGSGGPYALAAARALSRHSGLDAETIARESMRI
AAEICVYTNDHVTLETL

Specific function: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery

COG id: COG5405

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), peptidase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family. HslV subfamily

Homologues:

Organism=Escherichia coli, GI1790367, Length=172, Percent_Identity=56.9767441860465, Blast_Score=203, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLV_DESDA (B8J0S4)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480029.1
- MEROPS:   T01.007
- GeneID:   7285145
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1449
- HOGENOM:   HBG288822
- ProtClustDB:   PRK05456
- HAMAP:   MF_00248
- InterPro:   IPR022281
- InterPro:   IPR001353
- TIGRFAMs:   TIGR03692

Pfam domain/function: PF00227 Proteasome

EC number: 3.4.25.-

Molecular weight: Translated: 19244; Mature: 19244

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: ACT_SITE 6-6

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHATTILAVRKDGIVALAGDGQVTMGQTMIMKHAAQKVRRLHDGKILAGFAGATADAF
CCCCCEEEEEEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TLFELFESKLKEVRGHMVRAAVEMTKDWRKDKYLRKLEAMLLLADREHILVLSGTGDVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEC
PDDNVAAIGSGGPYALAAARALSRHSGLDAETIARESMRIAAEICVYTNDHVTLETL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCEEEEEEC
>Mature Secondary Structure
METHATTILAVRKDGIVALAGDGQVTMGQTMIMKHAAQKVRRLHDGKILAGFAGATADAF
CCCCCEEEEEEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TLFELFESKLKEVRGHMVRAAVEMTKDWRKDKYLRKLEAMLLLADREHILVLSGTGDVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEC
PDDNVAAIGSGGPYALAAARALSRHSGLDAETIARESMRIAAEICVYTNDHVTLETL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA