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The map label for this gene is gcvH [H]

Identifier: 220904061

GI number: 220904061

Start: 921584

End: 921970

Strand: Direct

Name: gcvH [H]

Synonym: Ddes_0787

Alternate gene names: 220904061

Gene position: 921584-921970 (Clockwise)

Preceding gene: 220904060

Following gene: 220904062

Centisome position: 32.07

GC content: 59.17

Gene sequence:

>387_bases
ATGGCTACCAATCCCTCGGACCTGTTGTACAGCAAGAGCCACGAATGGGTGCGCCTTGAAGGCGACGAAGCCGTGGTCGG
CATCACCCACTTTGCCCAGGAGTCCCTTGGCGACATTACCTATGTGGAACTGCCGCTTGTGGGCGATGAAGTCAGCGAAG
AAAGCGAATTCGGCTCCATTGAATCCGTCAAGGCCGCCAGCGACCTGGTATCGCCGGTGAGCGGTGATGTGATCGGTATC
AATACCGCCCTGGAGCAGGCGCCTGAAAAGTGCAACGCCGACCCCTACGGCGAAGGCTGGCTCATGCGCGTCAAATTTTC
CCGTAAGCCCGAAGGCCTGCTGGACGCAGCGGCTTACGAGTCCTTCTGCGCCACCGAAGGTCATTAG

Upstream 100 bases:

>100_bases
CGCTTGTGGAGCAGACCTGCCGCCCGTCAAATACGTGAAGGCTTGTGCGCCGAGAGGGCGCGTATTGTTCCCCCCAACTA
CATATTTCAGGAGAATGCAC

Downstream 100 bases:

>100_bases
GTCACAGTTTTTCCGGCGTCTGGTTTTGCCGCACGGCAGTATTGCCGTGCGGCGGCCAGGTCCGGAGGAATGAACCGGAC
AGAGCGGGCAGCGGCACGCC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MATNPSDLLYSKSHEWVRLEGDEAVVGITHFAQESLGDITYVELPLVGDEVSEESEFGSIESVKAASDLVSPVSGDVIGI
NTALEQAPEKCNADPYGEGWLMRVKFSRKPEGLLDAAAYESFCATEGH

Sequences:

>Translated_128_residues
MATNPSDLLYSKSHEWVRLEGDEAVVGITHFAQESLGDITYVELPLVGDEVSEESEFGSIESVKAASDLVSPVSGDVIGI
NTALEQAPEKCNADPYGEGWLMRVKFSRKPEGLLDAAAYESFCATEGH
>Mature_127_residues
ATNPSDLLYSKSHEWVRLEGDEAVVGITHFAQESLGDITYVELPLVGDEVSEESEFGSIESVKAASDLVSPVSGDVIGIN
TALEQAPEKCNADPYGEGWLMRVKFSRKPEGLLDAAAYESFCATEGH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=45.2173913043478, Blast_Score=103, Evalue=3e-23,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=44.3478260869565, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=48.062015503876, Blast_Score=117, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=123, Percent_Identity=43.9024390243902, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=41.6666666666667, Blast_Score=98, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=46.7213114754098, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=42.1487603305785, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13808; Mature: 13677

Theoretical pI: Translated: 3.98; Mature: 3.98

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNPSDLLYSKSHEWVRLEGDEAVVGITHFAQESLGDITYVELPLVGDEVSEESEFGSI
CCCCCCCCEECCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCC
ESVKAASDLVSPVSGDVIGINTALEQAPEKCNADPYGEGWLMRVKFSRKPEGLLDAAAYE
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
SFCATEGH
HHHCCCCC
>Mature Secondary Structure 
ATNPSDLLYSKSHEWVRLEGDEAVVGITHFAQESLGDITYVELPLVGDEVSEESEFGSI
CCCCCCCEECCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCC
ESVKAASDLVSPVSGDVIGINTALEQAPEKCNADPYGEGWLMRVKFSRKPEGLLDAAAYE
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
SFCATEGH
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA