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The map label for this gene is hyfB [H]

Identifier: 220904052

GI number: 220904052

Start: 910967

End: 913015

Strand: Reverse

Name: hyfB [H]

Synonym: Ddes_0778

Alternate gene names: 220904052

Gene position: 913015-910967 (Counterclockwise)

Preceding gene: 220904053

Following gene: 220904051

Centisome position: 31.77

GC content: 64.03

Gene sequence:

>2049_bases
ATGACGCTCTTTATCATAACCCTGCTTGTCCTGGCGGCGGCTGCCGCCGCCATGGCCCTGTTCGCGCTGCTGCCCTCTCC
GGCGGGCAGCGGCCGGGCCGCCAACCTGCTTGGCTCCTGTGGAGCCGCGGCGGGCTGTCTGGCGGGCCTGTGCGCGCTGG
CCTTCGCCCCCTGGGGCGAGGCTGTCTCCCTGCGCCTGCCCTGGGGCCTGCCCGTGGGGGCCTGTACTCTGGGTCTTGAC
CCCCTGAGCCGTATTTTCCTGCTGCCGGTTTTCGGCCTTGGCCTTGTGTGCGCCCTCTCCGGAGGCATGGCACTACGGCA
CACCCGCCCGCAGGAACACAACCTGGCGGCGCACTGGTTTTTTTACATCCTGCTGATTCTCGGGCTGGCGCTTGTCATGG
CGGCGCGTGATGCCGTACTTTTCATGCTGGCCTGGGAACTTATGTCTCTCGCGCCATTTTTTCTCATTGAATTTAATGAC
GGCGACCGTCAGGTGCGCGACGCCTCATGGGTCTACCTGGTGGCAGCCCACCTTGGGGCCGTGGCCCTTATGGCCTTTTT
TGTGCTGCTCTGGCAGAGCACGGGAACCACATCGCTTGACATCCTTCAGGGCGGCGCGGTCATGCAGGGGGTGGCGCGCG
ACGCCGGTTCCGGCGTGCTGACAGCCCTTTTTGCACTGGCCGTGCTCGGTTTCGGTGCCAAGGCCGGGCTTGTCCCCATG
CACGTCTGGCTGCCGGAGGCACACCCTGCCGCGCCAAGCCATGTTTCGGCCCTGCTTTCCGGAGCCATGATCAACGCGGG
CCTGTACGGAATCATTCGCAGCCTCAGCCTGCTGGCCGCGCCCGGCGCGGCTCCCGAATGGTGGGGCTGGACCCTGCTGC
TGCTGGGCCTTGCCACCGGCCTGATCGGCATTCTGAAAGCGCTGGGCCAGAGCAACCTCAAACGCCTGCTGGCCTACTCC
AGCGTGGAAAACATGGGACTTATGCTCATGGGCGTGGGCGCAGGTCTCATAGGCCTGGCCTGCGGCAATGCATGGATAGC
CACCCTCGGTTTTGCGGGCAGCCTTATGCACATGCTGAACCACGCCGGTTTCAAGGGACTGCTTTTTCTGTCCGCCGGAG
AAGTGCTGCATGCCACAGGCACTGTGCGTATGGAGCAGTTGGGCGGCCTGCAAAAACGCCTGCCCCTGCTTGGCGCGGCC
TTTGCCCTTGGAGCAGCGGCCATTGCCTGCCTGCCGCCTCTTAACGGTTTTGCAGGTGAGCTTGTGCTGGCGCTTTCGCT
GCTGGACGGGCCAAGCCTGCCCGGCGTGGAGCGGCAGGTGGGGCTTTTGCTGGCGCTGGTAACCCTTGCTCTCATCAGTG
GCCTTGCCGCCGCCCTGTACGCCAAGGCCTACGGCATCACCTTTCTTGGCGAGCCACGTTCGGGCTTTGCGGCCAATGCC
CGCCCCGCAGGCTGGCGAACCCTCTGGCCTCTCGCCCTGCCCGCGGCAGTCTGTGTGCTTGGCGGTCTGGCGGCTCCGCT
CTTTTTTGACCTCGCCTCCATGACGGGACTGGCCGCCCTGCCCATGCCCAACGACATGCAGGCCGGTGCCCTGGCAGGAC
AAGAACAGATGCGCGCCAGCCTTGCCATGGTGTCGCTGGTCGGCTCCATTGCCCTGGGCCTTGCCCTGGCAGGCATTGTC
CTGCGCAAACGGCTGCTCAAAAGGCGCAGCGTGGGCAGTATGCCCACCTGGGGCTGCGGCTATCAGGGCGGTACGGCCCG
CATCCAGTACACTGATGCGGGATTTTCCGAACCGCTCGGCAAGATATTCGCGCCCGGCATGGGCCTGAAAGTGCGCATGC
AGCTGGATTCACGTCTGTTCCCCGACAAGGGGGAAATGAGCGTTTCCGCTCCTGACCGCCTGCGCCGCTCCATCTACGAC
CCGCTTTTTGAAGGCATAGAGCGCCTGTGCAATGCCTGCAAGATCATGCAACACGGCAAAATACATCTTTATATTCTTTA
CATTCTGGCCACAGTTGTGGGTCTGCTTGTATGGGGGCTGAGCGCATGA

Upstream 100 bases:

>100_bases
AGGTCAAGCTGGAAGACGGTTTGAAAAAGACCATCGCCTACTTTGACAGCATGTTAAAACTGGGCATGGCCTGACCACTG
CCCGAAAGCCACCCTATGCC

Downstream 100 bases:

>100_bases
GACAAGACATAGCCGTCTATTTCGTGCAGTTTGCGCTGGCCCTTGTTCTGGCTCCGCTGCTGCCCGGCATTATCAACAGG
GTCAAGGCCAAGTTTGCAGG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MTLFIITLLVLAAAAAAMALFALLPSPAGSGRAANLLGSCGAAAGCLAGLCALAFAPWGEAVSLRLPWGLPVGACTLGLD
PLSRIFLLPVFGLGLVCALSGGMALRHTRPQEHNLAAHWFFYILLILGLALVMAARDAVLFMLAWELMSLAPFFLIEFND
GDRQVRDASWVYLVAAHLGAVALMAFFVLLWQSTGTTSLDILQGGAVMQGVARDAGSGVLTALFALAVLGFGAKAGLVPM
HVWLPEAHPAAPSHVSALLSGAMINAGLYGIIRSLSLLAAPGAAPEWWGWTLLLLGLATGLIGILKALGQSNLKRLLAYS
SVENMGLMLMGVGAGLIGLACGNAWIATLGFAGSLMHMLNHAGFKGLLFLSAGEVLHATGTVRMEQLGGLQKRLPLLGAA
FALGAAAIACLPPLNGFAGELVLALSLLDGPSLPGVERQVGLLLALVTLALISGLAAALYAKAYGITFLGEPRSGFAANA
RPAGWRTLWPLALPAAVCVLGGLAAPLFFDLASMTGLAALPMPNDMQAGALAGQEQMRASLAMVSLVGSIALGLALAGIV
LRKRLLKRRSVGSMPTWGCGYQGGTARIQYTDAGFSEPLGKIFAPGMGLKVRMQLDSRLFPDKGEMSVSAPDRLRRSIYD
PLFEGIERLCNACKIMQHGKIHLYILYILATVVGLLVWGLSA

Sequences:

>Translated_682_residues
MTLFIITLLVLAAAAAAMALFALLPSPAGSGRAANLLGSCGAAAGCLAGLCALAFAPWGEAVSLRLPWGLPVGACTLGLD
PLSRIFLLPVFGLGLVCALSGGMALRHTRPQEHNLAAHWFFYILLILGLALVMAARDAVLFMLAWELMSLAPFFLIEFND
GDRQVRDASWVYLVAAHLGAVALMAFFVLLWQSTGTTSLDILQGGAVMQGVARDAGSGVLTALFALAVLGFGAKAGLVPM
HVWLPEAHPAAPSHVSALLSGAMINAGLYGIIRSLSLLAAPGAAPEWWGWTLLLLGLATGLIGILKALGQSNLKRLLAYS
SVENMGLMLMGVGAGLIGLACGNAWIATLGFAGSLMHMLNHAGFKGLLFLSAGEVLHATGTVRMEQLGGLQKRLPLLGAA
FALGAAAIACLPPLNGFAGELVLALSLLDGPSLPGVERQVGLLLALVTLALISGLAAALYAKAYGITFLGEPRSGFAANA
RPAGWRTLWPLALPAAVCVLGGLAAPLFFDLASMTGLAALPMPNDMQAGALAGQEQMRASLAMVSLVGSIALGLALAGIV
LRKRLLKRRSVGSMPTWGCGYQGGTARIQYTDAGFSEPLGKIFAPGMGLKVRMQLDSRLFPDKGEMSVSAPDRLRRSIYD
PLFEGIERLCNACKIMQHGKIHLYILYILATVVGLLVWGLSA
>Mature_681_residues
TLFIITLLVLAAAAAAMALFALLPSPAGSGRAANLLGSCGAAAGCLAGLCALAFAPWGEAVSLRLPWGLPVGACTLGLDP
LSRIFLLPVFGLGLVCALSGGMALRHTRPQEHNLAAHWFFYILLILGLALVMAARDAVLFMLAWELMSLAPFFLIEFNDG
DRQVRDASWVYLVAAHLGAVALMAFFVLLWQSTGTTSLDILQGGAVMQGVARDAGSGVLTALFALAVLGFGAKAGLVPMH
VWLPEAHPAAPSHVSALLSGAMINAGLYGIIRSLSLLAAPGAAPEWWGWTLLLLGLATGLIGILKALGQSNLKRLLAYSS
VENMGLMLMGVGAGLIGLACGNAWIATLGFAGSLMHMLNHAGFKGLLFLSAGEVLHATGTVRMEQLGGLQKRLPLLGAAF
ALGAAAIACLPPLNGFAGELVLALSLLDGPSLPGVERQVGLLLALVTLALISGLAAALYAKAYGITFLGEPRSGFAANAR
PAGWRTLWPLALPAAVCVLGGLAAPLFFDLASMTGLAALPMPNDMQAGALAGQEQMRASLAMVSLVGSIALGLALAGIVL
RKRLLKRRSVGSMPTWGCGYQGGTARIQYTDAGFSEPLGKIFAPGMGLKVRMQLDSRLFPDKGEMSVSAPDRLRRSIYDP
LFEGIERLCNACKIMQHGKIHLYILYILATVVGLLVWGLSA

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=190, Percent_Identity=32.1052631578947, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI251831116, Length=305, Percent_Identity=28.1967213114754, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788827, Length=613, Percent_Identity=34.584013050571, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI2367154, Length=539, Percent_Identity=34.3228200371057, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1788831, Length=341, Percent_Identity=31.0850439882698, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788829, Length=440, Percent_Identity=30, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788613, Length=360, Percent_Identity=26.6666666666667, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788614, Length=407, Percent_Identity=29.4840294840295, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI145693160, Length=312, Percent_Identity=28.525641025641, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70951; Mature: 70819

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFIITLLVLAAAAAAMALFALLPSPAGSGRAANLLGSCGAAAGCLAGLCALAFAPWGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCC
AVSLRLPWGLPVGACTLGLDPLSRIFLLPVFGLGLVCALSGGMALRHTRPQEHNLAAHWF
EEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHH
FYILLILGLALVMAARDAVLFMLAWELMSLAPFFLIEFNDGDRQVRDASWVYLVAAHLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
VALMAFFVLLWQSTGTTSLDILQGGAVMQGVARDAGSGVLTALFALAVLGFGAKAGLVPM
HHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
HVWLPEAHPAAPSHVSALLSGAMINAGLYGIIRSLSLLAAPGAAPEWWGWTLLLLGLATG
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIGILKALGQSNLKRLLAYSSVENMGLMLMGVGAGLIGLACGNAWIATLGFAGSLMHMLN
HHHHHHHHCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
HAGFKGLLFLSAGEVLHATGTVRMEQLGGLQKRLPLLGAAFALGAAAIACLPPLNGFAGE
HCCCCEEEEEECCCEEECCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LVLALSLLDGPSLPGVERQVGLLLALVTLALISGLAAALYAKAYGITFLGEPRSGFAANA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCC
RPAGWRTLWPLALPAAVCVLGGLAAPLFFDLASMTGLAALPMPNDMQAGALAGQEQMRAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHCCHHHHHHH
LAMVSLVGSIALGLALAGIVLRKRLLKRRSVGSMPTWGCGYQGGTARIQYTDAGFSEPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
KIFAPGMGLKVRMQLDSRLFPDKGEMSVSAPDRLRRSIYDPLFEGIERLCNACKIMQHGK
HHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHLYILYILATVVGLLVWGLSA
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLFIITLLVLAAAAAAMALFALLPSPAGSGRAANLLGSCGAAAGCLAGLCALAFAPWGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCC
AVSLRLPWGLPVGACTLGLDPLSRIFLLPVFGLGLVCALSGGMALRHTRPQEHNLAAHWF
EEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHH
FYILLILGLALVMAARDAVLFMLAWELMSLAPFFLIEFNDGDRQVRDASWVYLVAAHLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
VALMAFFVLLWQSTGTTSLDILQGGAVMQGVARDAGSGVLTALFALAVLGFGAKAGLVPM
HHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
HVWLPEAHPAAPSHVSALLSGAMINAGLYGIIRSLSLLAAPGAAPEWWGWTLLLLGLATG
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIGILKALGQSNLKRLLAYSSVENMGLMLMGVGAGLIGLACGNAWIATLGFAGSLMHMLN
HHHHHHHHCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
HAGFKGLLFLSAGEVLHATGTVRMEQLGGLQKRLPLLGAAFALGAAAIACLPPLNGFAGE
HCCCCEEEEEECCCEEECCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LVLALSLLDGPSLPGVERQVGLLLALVTLALISGLAAALYAKAYGITFLGEPRSGFAANA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCC
RPAGWRTLWPLALPAAVCVLGGLAAPLFFDLASMTGLAALPMPNDMQAGALAGQEQMRAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHCCHHHHHHH
LAMVSLVGSIALGLALAGIVLRKRLLKRRSVGSMPTWGCGYQGGTARIQYTDAGFSEPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
KIFAPGMGLKVRMQLDSRLFPDKGEMSVSAPDRLRRSIYDPLFEGIERLCNACKIMQHGK
HHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHLYILYILATVVGLLVWGLSA
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]