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The map label for this gene is rimN [H]

Identifier: 220904022

GI number: 220904022

Start: 882500

End: 883171

Strand: Direct

Name: rimN [H]

Synonym: Ddes_0748

Alternate gene names: 220904022

Gene position: 882500-883171 (Clockwise)

Preceding gene: 220904020

Following gene: 220904024

Centisome position: 30.71

GC content: 66.37

Gene sequence:

>672_bases
ATGTCTCTCCAAATAGTCTGCCGCGATCTGCAAGAGGCCGTTGCCTGCCTGCGCGGCGGTCATGCACTGATTTTTCCGAC
CGAAACATTTTATGGTCTCGGCTGCCTGGCTTCCGATGCCGCTGCCGTGAGCCGAATCTATCAGTTCAAGCGGCGTCCCG
TCCATAAGCCCTTGCCCCTGCTGGCCGCCAGTGAGGGGCAGGTGCGCTCGGTAGCGCAGCTTGAGGCCATGCCGGAACCC
TTGCGGGCCTTCTGGCCCGGGCCTCTTACCGTGCTGCTGCCCGCAAGGCAAGGTCTGCCTTCGCAACTGGTGAATGAGGC
GGGGCAGGTGGCGGTGCGTGTAACCCCCCATCCCGTAGCTGCGGCTCTGGCGCAAGGGGCTGGCGGCCCGCTTACGGCAA
GCAGCGCCAATCTTAACGGGCGGTCGCCCGTATGCGCTGTGGCGGACCTGGATCCGCAACTGCTGGCCGCGCTGCAGGAT
CTGGGGCAGTGCCCCTGTGTGGTCGCTGAAGGCCCACAGCCGCAAGGCGGTGTGCCTTCGTCCCTTGTGGAGCTTTTGCC
GGAACAGGGCGAACGCCGTGCGGGCGCCTCATGCCGGCGGCTGCGCATTATCCGGTCCGGGGCGGTAAGCCGGGCTGCGC
TGGAGGCGGCGGGCTTTACGGTGGAGCCGTAA

Upstream 100 bases:

>100_bases
CCGCCAGCGTTTGATGCGCGTAAGGTAGTGGGCTGCGGCGTCCTTCCAGAAAGGTGTTTTCAACTTGCCCACACTGATGC
ACCGTAAAGGTTTGCCTGCC

Downstream 100 bases:

>100_bases
GCAATGGGCCTGAGGGCAGGGGGCGGTCAGTTTTTGCTGAAGGCCGTGAGGCGGGCCAGAATATCGTCCAGAAAATGCGC
AAAATTTTCCACAGTGGCGG

Product: Sua5/YciO/YrdC/YwlC family protein

Products: NA

Alternate protein names: t(6)A37 threonylcarbamoyladenosine biosynthesis protein RimN [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSLQIVCRDLQEAVACLRGGHALIFPTETFYGLGCLASDAAAVSRIYQFKRRPVHKPLPLLAASEGQVRSVAQLEAMPEP
LRAFWPGPLTVLLPARQGLPSQLVNEAGQVAVRVTPHPVAAALAQGAGGPLTASSANLNGRSPVCAVADLDPQLLAALQD
LGQCPCVVAEGPQPQGGVPSSLVELLPEQGERRAGASCRRLRIIRSGAVSRAALEAAGFTVEP

Sequences:

>Translated_223_residues
MSLQIVCRDLQEAVACLRGGHALIFPTETFYGLGCLASDAAAVSRIYQFKRRPVHKPLPLLAASEGQVRSVAQLEAMPEP
LRAFWPGPLTVLLPARQGLPSQLVNEAGQVAVRVTPHPVAAALAQGAGGPLTASSANLNGRSPVCAVADLDPQLLAALQD
LGQCPCVVAEGPQPQGGVPSSLVELLPEQGERRAGASCRRLRIIRSGAVSRAALEAAGFTVEP
>Mature_222_residues
SLQIVCRDLQEAVACLRGGHALIFPTETFYGLGCLASDAAAVSRIYQFKRRPVHKPLPLLAASEGQVRSVAQLEAMPEPL
RAFWPGPLTVLLPARQGLPSQLVNEAGQVAVRVTPHPVAAALAQGAGGPLTASSANLNGRSPVCAVADLDPQLLAALQDL
GQCPCVVAEGPQPQGGVPSSLVELLPEQGERRAGASCRRLRIIRSGAVSRAALEAAGFTVEP

Specific function: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine [H]

COG id: COG0009

COG function: function code J; Putative translation factor (SUA5)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367210, Length=150, Percent_Identity=36, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17537239, Length=219, Percent_Identity=26.9406392694064, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321269, Length=198, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707274, Length=131, Percent_Identity=31.2977099236641, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR006070 [H]

Pfam domain/function: PF01300 Sua5_yciO_yrdC [H]

EC number: NA

Molecular weight: Translated: 23216; Mature: 23085

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQIVCRDLQEAVACLRGGHALIFPTETFYGLGCLASDAAAVSRIYQFKRRPVHKPLPL
CCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LAASEGQVRSVAQLEAMPEPLRAFWPGPLTVLLPARQGLPSQLVNEAGQVAVRVTPHPVA
EECCCCCHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCEEEEECCCHHH
AALAQGAGGPLTASSANLNGRSPVCAVADLDPQLLAALQDLGQCPCVVAEGPQPQGGVPS
HHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCHH
SLVELLPEQGERRAGASCRRLRIIRSGAVSRAALEAAGFTVEP
HHHHHHHHCCCHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLQIVCRDLQEAVACLRGGHALIFPTETFYGLGCLASDAAAVSRIYQFKRRPVHKPLPL
CHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LAASEGQVRSVAQLEAMPEPLRAFWPGPLTVLLPARQGLPSQLVNEAGQVAVRVTPHPVA
EECCCCCHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCEEEEECCCHHH
AALAQGAGGPLTASSANLNGRSPVCAVADLDPQLLAALQDLGQCPCVVAEGPQPQGGVPS
HHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCHH
SLVELLPEQGERRAGASCRRLRIIRSGAVSRAALEAAGFTVEP
HHHHHHHHCCCHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]