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The map label for this gene is hisJ [H]

Identifier: 220903864

GI number: 220903864

Start: 714067

End: 714810

Strand: Direct

Name: hisJ [H]

Synonym: Ddes_0589

Alternate gene names: 220903864

Gene position: 714067-714810 (Clockwise)

Preceding gene: 220903863

Following gene: 220903865

Centisome position: 24.85

GC content: 55.91

Gene sequence:

>744_bases
ATGATACGTCGTTTTGCTTTCGCCCTGCTGGTTCTGACCCTGTTTTGCGGTCAGGCTTTGGCCGCTGAAAAATATGTTGT
GGCTACCGACTGCACCTGGCCGCCTATGGAGCTTCTTGATGAAAACAAGCAGCCCGTCGGCTTTGACGTGGATTTTATTA
CTGAAGTGGGCAAGGCCGCCGGTTTTGAGGTGGACGTGCGCAATATCGCGTGGGACGGTATTTTTGGCGGCGTAGCCACC
GGCCAGTATGATATTGTGGCTGCTGCCACAACCATCACGCCCGAGCGCCAGAAGCAGTTTGATTTTTCCGATCCCTACTA
TGAGGTGGCCCAGGCTGTGGTGCTGCCTGCGGGGCAGAACATAAAGAGCCTTGACGACCTCAAGGGCAAAAAAGTGGGCG
GCCAGATCGGCACCACCGGCGTTTTTGTTATCCGTAAGTCCGGTGTGCCTGTGGATCTCAAGGAATATGACGACGTGGGC
CTGGCTATCCAGGACATGCTCGGCGGCCGCATTGACGCCGTGATCTGCGACGAGCCTGTGGCCATGTATTACGCTAACAA
AAAGGCCGACACTGCCGGCAAGCTGAACCTTTCGTTCAAGACCGCCGAAAAGGAATACTACGGCTTTACCGTGCGCAAGG
GCCGTACGGACCTGGTGGAAAAACTCAACAAGGGTATCAAGGCCGTCAAGGCTTCGGGCGTAGAGGCCGAGCTGCTTGAG
AAGTGGATGGGCAAGGCCGACTAA

Upstream 100 bases:

>100_bases
GAGGAAGCCAGGATAATTGAAAAGTGGATGGGCAAATCCAACTAGCCCACAGGGGACGCGCCGGTACTTCCGGCGGAGTC
CGTCGCATGAAGGAGGTTTC

Downstream 100 bases:

>100_bases
ACCGGACCAGCCATTATGGTTTCATGGGAGTCCGGGCCGTGCGGCCCGGACTCCGCAGCCGTATTGATCTGAAGGATTGT
GATGCACGATAAAAAAGACG

Product: family 3 extracellular solute-binding protein

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MIRRFAFALLVLTLFCGQALAAEKYVVATDCTWPPMELLDENKQPVGFDVDFITEVGKAAGFEVDVRNIAWDGIFGGVAT
GQYDIVAAATTITPERQKQFDFSDPYYEVAQAVVLPAGQNIKSLDDLKGKKVGGQIGTTGVFVIRKSGVPVDLKEYDDVG
LAIQDMLGGRIDAVICDEPVAMYYANKKADTAGKLNLSFKTAEKEYYGFTVRKGRTDLVEKLNKGIKAVKASGVEAELLE
KWMGKAD

Sequences:

>Translated_247_residues
MIRRFAFALLVLTLFCGQALAAEKYVVATDCTWPPMELLDENKQPVGFDVDFITEVGKAAGFEVDVRNIAWDGIFGGVAT
GQYDIVAAATTITPERQKQFDFSDPYYEVAQAVVLPAGQNIKSLDDLKGKKVGGQIGTTGVFVIRKSGVPVDLKEYDDVG
LAIQDMLGGRIDAVICDEPVAMYYANKKADTAGKLNLSFKTAEKEYYGFTVRKGRTDLVEKLNKGIKAVKASGVEAELLE
KWMGKAD
>Mature_247_residues
MIRRFAFALLVLTLFCGQALAAEKYVVATDCTWPPMELLDENKQPVGFDVDFITEVGKAAGFEVDVRNIAWDGIFGGVAT
GQYDIVAAATTITPERQKQFDFSDPYYEVAQAVVLPAGQNIKSLDDLKGKKVGGQIGTTGVFVIRKSGVPVDLKEYDDVG
LAIQDMLGGRIDAVICDEPVAMYYANKKADTAGKLNLSFKTAEKEYYGFTVRKGRTDLVEKLNKGIKAVKASGVEAELLE
KWMGKAD

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=221, Percent_Identity=34.3891402714932, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=33.1983805668016, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787088, Length=235, Percent_Identity=34.8936170212766, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=32.258064516129, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788648, Length=225, Percent_Identity=25.7777777777778, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26938; Mature: 26938

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRFAFALLVLTLFCGQALAAEKYVVATDCTWPPMELLDENKQPVGFDVDFITEVGKAA
CHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
GFEVDVRNIAWDGIFGGVATGQYDIVAAATTITPERQKQFDFSDPYYEVAQAVVLPAGQN
CCEEEHHHEEECCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCC
IKSLDDLKGKKVGGQIGTTGVFVIRKSGVPVDLKEYDDVGLAIQDMLGGRIDAVICDEPV
CCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCEEEEEECCCE
AMYYANKKADTAGKLNLSFKTAEKEYYGFTVRKGRTDLVEKLNKGIKAVKASGVEAELLE
EEEEECCCCCCCCEEEEEEEECCHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHH
KWMGKAD
HHCCCCC
>Mature Secondary Structure
MIRRFAFALLVLTLFCGQALAAEKYVVATDCTWPPMELLDENKQPVGFDVDFITEVGKAA
CHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
GFEVDVRNIAWDGIFGGVATGQYDIVAAATTITPERQKQFDFSDPYYEVAQAVVLPAGQN
CCEEEHHHEEECCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCC
IKSLDDLKGKKVGGQIGTTGVFVIRKSGVPVDLKEYDDVGLAIQDMLGGRIDAVICDEPV
CCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCEEEEEECCCE
AMYYANKKADTAGKLNLSFKTAEKEYYGFTVRKGRTDLVEKLNKGIKAVKASGVEAELLE
EEEEECCCCCCCCEEEEEEEECCHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHH
KWMGKAD
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]