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The map label for this gene is hisJ [H]

Identifier: 220903863

GI number: 220903863

Start: 713268

End: 714011

Strand: Direct

Name: hisJ [H]

Synonym: Ddes_0588

Alternate gene names: 220903863

Gene position: 713268-714011 (Clockwise)

Preceding gene: 220903862

Following gene: 220903864

Centisome position: 24.82

GC content: 55.65

Gene sequence:

>744_bases
ATGCTTCGCCGAATGACACTCGCCTTCGCTGGTTTTGTTCTGATGTGCAGCTCTGCTATGGCCGCTGAAAAAATCGTGGT
CGCCAGCGACTGTACCTGGCCACCCATGGAAATGCTTGATGCCAACAAGAAACCCGAAGGGTACTCCAGCGATTACCTGC
GGGCCGTAGGCAAGGCCGCAGGTCTTGATATGGACATCCGCAACGTGGCCTGGGACGGTATTTTCGGCGGTGTGGCCACC
GGGCAGTACGATGTTGTGGCCTCTTCCGTCACCATTACGCCCGAACGCCAGAAACAGTTTGACTTCACCGAGCCTTACTA
TGAGGTTGTGCAGGCCGTGGTGCTGCCCGAAGGGCAGAGCGTCAAGAGCCTGGCCGACCTCAAGGGCAAAAAGGTGGGCG
GCCAGATCGGCACCACCGGCATCTTTGTGACCCGTAAGGCCGATGTCGGCGCTGACATCAAGGAATATGACGACGTGGGC
CTGGCTATTCAGGACCTGGTCAATGGCCGCATTGATGCGGTGGTCTGTGATGACCCGGTAGCCATGTACTATGTGAACAA
AAAGGCCGACACCGCCGGCAAGCTGAACCTTTCTTACAAGACCGACGAAAAGGAATACTACGGCTTTACCGTGCGCAAGG
GCCGCAAGGACCTTTTGGACAAGCTCAATAAAGGCATCAGAGAAGTAAAGGCATCCGGTGAGGAAGCCAGGATAATTGAA
AAGTGGATGGGCAAATCCAACTAG

Upstream 100 bases:

>100_bases
ATTCAGCCGTGGCCTTCCGCCGCGGGGAATATCCCGGGGCAGGCTTTTCCACTGGCGCTGTGGGCGGCGGGCTTTGACGG
AAACCATTTAAGGAGGTCCC

Downstream 100 bases:

>100_bases
CCCACAGGGGACGCGCCGGTACTTCCGGCGGAGTCCGTCGCATGAAGGAGGTTTCATGATACGTCGTTTTGCTTTCGCCC
TGCTGGTTCTGACCCTGTTT

Product: family 3 extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLRRMTLAFAGFVLMCSSAMAAEKIVVASDCTWPPMEMLDANKKPEGYSSDYLRAVGKAAGLDMDIRNVAWDGIFGGVAT
GQYDVVASSVTITPERQKQFDFTEPYYEVVQAVVLPEGQSVKSLADLKGKKVGGQIGTTGIFVTRKADVGADIKEYDDVG
LAIQDLVNGRIDAVVCDDPVAMYYVNKKADTAGKLNLSYKTDEKEYYGFTVRKGRKDLLDKLNKGIREVKASGEEARIIE
KWMGKSN

Sequences:

>Translated_247_residues
MLRRMTLAFAGFVLMCSSAMAAEKIVVASDCTWPPMEMLDANKKPEGYSSDYLRAVGKAAGLDMDIRNVAWDGIFGGVAT
GQYDVVASSVTITPERQKQFDFTEPYYEVVQAVVLPEGQSVKSLADLKGKKVGGQIGTTGIFVTRKADVGADIKEYDDVG
LAIQDLVNGRIDAVVCDDPVAMYYVNKKADTAGKLNLSYKTDEKEYYGFTVRKGRKDLLDKLNKGIREVKASGEEARIIE
KWMGKSN
>Mature_247_residues
MLRRMTLAFAGFVLMCSSAMAAEKIVVASDCTWPPMEMLDANKKPEGYSSDYLRAVGKAAGLDMDIRNVAWDGIFGGVAT
GQYDVVASSVTITPERQKQFDFTEPYYEVVQAVVLPEGQSVKSLADLKGKKVGGQIGTTGIFVTRKADVGADIKEYDDVG
LAIQDLVNGRIDAVVCDDPVAMYYVNKKADTAGKLNLSYKTDEKEYYGFTVRKGRKDLLDKLNKGIREVKASGEEARIIE
KWMGKSN

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=249, Percent_Identity=34.9397590361446, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787088, Length=240, Percent_Identity=34.5833333333333, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=30.327868852459, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=25, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27081; Mature: 27081

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRMTLAFAGFVLMCSSAMAAEKIVVASDCTWPPMEMLDANKKPEGYSSDYLRAVGKAA
CCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
GLDMDIRNVAWDGIFGGVATGQYDVVASSVTITPERQKQFDFTEPYYEVVQAVVLPEGQS
CCCCHHHHHHHCCHHCCCCCCCEEEEEEEEEECCCHHCCCCCCCHHHHHHHHHHCCCCCC
VKSLADLKGKKVGGQIGTTGIFVTRKADVGADIKEYDDVGLAIQDLVNGRIDAVVCDDPV
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCE
AMYYVNKKADTAGKLNLSYKTDEKEYYGFTVRKGRKDLLDKLNKGIREVKASGEEARIIE
EEEEEECCCCCCCEEEEEEECCCCHHCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
KWMGKSN
HHCCCCC
>Mature Secondary Structure
MLRRMTLAFAGFVLMCSSAMAAEKIVVASDCTWPPMEMLDANKKPEGYSSDYLRAVGKAA
CCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
GLDMDIRNVAWDGIFGGVATGQYDVVASSVTITPERQKQFDFTEPYYEVVQAVVLPEGQS
CCCCHHHHHHHCCHHCCCCCCCEEEEEEEEEECCCHHCCCCCCCHHHHHHHHHHCCCCCC
VKSLADLKGKKVGGQIGTTGIFVTRKADVGADIKEYDDVGLAIQDLVNGRIDAVVCDDPV
HHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCE
AMYYVNKKADTAGKLNLSYKTDEKEYYGFTVRKGRKDLLDKLNKGIREVKASGEEARIIE
EEEEEECCCCCCCEEEEEEECCCCHHCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
KWMGKSN
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]