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The map label for this gene is leuB [H]

Identifier: 220903786

GI number: 220903786

Start: 615630

End: 616703

Strand: Direct

Name: leuB [H]

Synonym: Ddes_0509

Alternate gene names: 220903786

Gene position: 615630-616703 (Clockwise)

Preceding gene: 220903784

Following gene: 220903788

Centisome position: 21.42

GC content: 59.4

Gene sequence:

>1074_bases
ATGAAAAAGACTATCTGCCTGTTGCCCGGCGACGGTATCGGCCCGGAAATAGTAGCCCAGGGCGTCAAGGTTCTGGAAGC
CACGGCTGAAAAATCCGGCCATCAGTTTGTTTTTGAGACGGCCCTTATCGGCGGGGCGGCCATTGACGCCGCGGGCGATC
CCTTACCTGAGGACACAGTACGGCTGTGCCGCACTGCTGATGCCGTGTATCTCGGCGCGGTGGGCGGTCCCAAATGGGAT
GGCATGAGCCAGGATCTGCGGCCCGAAAAAGGGCTGCTGCGCATTCGCAAAGAGCTTGGCCTGTTTGCCAATCTGCGCCC
GGCCATGCTTTTGCCCGAGCTTGCGGGGGCCTGCCTGCTGCGGGCCGACATTGCGGCCAGGGGGCTTGACCTTATGGTGG
TGCGCGAACTGACCGGTGATGTGTATTTCGGCGAACCACGCGGCATTGAAGTGCGCGACGGCCTGCGCACCGGCTACAAT
ACCATGCTGTATACTGAGGAAGAGATCCGTCGTATCGCCGTGATTGCGTTTGAAACGGCCCGCAAGCGCAGACGCCGGGT
ATGCTCGGTGGAGAAGAGCAACGTGCTGGAATGCTCGCGCCTCTGGAAAGAAGTTGTCATGGAGGTGCACCGGGAATATG
CCGATGTGGAACTGAGCCACATGTATGTGGACAATGCCGCCATGCAGCTTGTGCGCGATCCCTCGCAGTTTGATGTGATT
CTGACCGGCAATATTTTTGGCGATATCCTGTCGGACGAGGCTTCGGTCATTACCGGTTCTCTGGGCATGCTGCCTTCGGC
CTCTACCGGGGCAGAAGGGCCGGGGCTGTTTGAACCCATACACGGCTCGGCTCCGGATATAGCGGGCCATGATATAGCCA
ATCCCCTGGCGACCATTCTTTCAGCCGCCATGATGCTGCGGCTGGGCTTTGATATGGCCGAAGAGGCTGACCATATTGAA
ACGGCCGTGCGCAAGGCCCTGGCTGACGGTTTTCGCACTGCCGACATTATGGAGCCCGGTAAAGAACTGCTGGGCTGCGC
TGCCATGGGTGATAAGGTGGTGGAACGCCTGTAG

Upstream 100 bases:

>100_bases
AAGGTCCACGCGGCGACTGGAAAGCTTGAACCGGGGAAGGAGCCATCGCGGCATGTACGCGCCTTCCTAGCGGGATGATC
AAAATATGCAAGGATGTTTC

Downstream 100 bases:

>100_bases
GGCAATTTGCATTGTGCTGGCTGGCGCGCGAGGAAACGTCCGCCTTCGGAGGCCCGATCATCTTCCGCCGCTGCCTGCAG
GGCTTGTGGATGAAAACATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKTICLLPGDGIGPEIVAQGVKVLEATAEKSGHQFVFETALIGGAAIDAAGDPLPEDTVRLCRTADAVYLGAVGGPKWD
GMSQDLRPEKGLLRIRKELGLFANLRPAMLLPELAGACLLRADIAARGLDLMVVRELTGDVYFGEPRGIEVRDGLRTGYN
TMLYTEEEIRRIAVIAFETARKRRRRVCSVEKSNVLECSRLWKEVVMEVHREYADVELSHMYVDNAAMQLVRDPSQFDVI
LTGNIFGDILSDEASVITGSLGMLPSASTGAEGPGLFEPIHGSAPDIAGHDIANPLATILSAAMMLRLGFDMAEEADHIE
TAVRKALADGFRTADIMEPGKELLGCAAMGDKVVERL

Sequences:

>Translated_357_residues
MKKTICLLPGDGIGPEIVAQGVKVLEATAEKSGHQFVFETALIGGAAIDAAGDPLPEDTVRLCRTADAVYLGAVGGPKWD
GMSQDLRPEKGLLRIRKELGLFANLRPAMLLPELAGACLLRADIAARGLDLMVVRELTGDVYFGEPRGIEVRDGLRTGYN
TMLYTEEEIRRIAVIAFETARKRRRRVCSVEKSNVLECSRLWKEVVMEVHREYADVELSHMYVDNAAMQLVRDPSQFDVI
LTGNIFGDILSDEASVITGSLGMLPSASTGAEGPGLFEPIHGSAPDIAGHDIANPLATILSAAMMLRLGFDMAEEADHIE
TAVRKALADGFRTADIMEPGKELLGCAAMGDKVVERL
>Mature_357_residues
MKKTICLLPGDGIGPEIVAQGVKVLEATAEKSGHQFVFETALIGGAAIDAAGDPLPEDTVRLCRTADAVYLGAVGGPKWD
GMSQDLRPEKGLLRIRKELGLFANLRPAMLLPELAGACLLRADIAARGLDLMVVRELTGDVYFGEPRGIEVRDGLRTGYN
TMLYTEEEIRRIAVIAFETARKRRRRVCSVEKSNVLECSRLWKEVVMEVHREYADVELSHMYVDNAAMQLVRDPSQFDVI
LTGNIFGDILSDEASVITGSLGMLPSASTGAEGPGLFEPIHGSAPDIAGHDIANPLATILSAAMMLRLGFDMAEEADHIE
TAVRKALADGFRTADIMEPGKELLGCAAMGDKVVERL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=334, Percent_Identity=35.9281437125749, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI28178816, Length=311, Percent_Identity=28.9389067524116, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178821, Length=311, Percent_Identity=28.9389067524116, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4758582, Length=356, Percent_Identity=26.123595505618, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI28178838, Length=361, Percent_Identity=26.0387811634349, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28178819, Length=147, Percent_Identity=37.4149659863946, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081683, Length=350, Percent_Identity=53.7142857142857, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788101, Length=349, Percent_Identity=36.3896848137536, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1787381, Length=411, Percent_Identity=26.5206812652068, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=34.6368715083799, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=30, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=338, Percent_Identity=29.8816568047337, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=334, Percent_Identity=28.7425149700599, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319830, Length=350, Percent_Identity=46.8571428571429, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=38.10888252149, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=342, Percent_Identity=33.9181286549708, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=338, Percent_Identity=30.7692307692308, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=34.2105263157895, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=34.2105263157895, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=30.6010928961749, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161078635, Length=346, Percent_Identity=29.7687861271676, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=28.9085545722714, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=28.9085545722714, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078633, Length=346, Percent_Identity=29.7687861271676, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24650122, Length=346, Percent_Identity=29.7687861271676, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078637, Length=346, Percent_Identity=29.7687861271676, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078639, Length=341, Percent_Identity=30.2052785923754, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130355, Length=170, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38590; Mature: 38590

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTICLLPGDGIGPEIVAQGVKVLEATAEKSGHQFVFETALIGGAAIDAAGDPLPEDTV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHCCCCCCCCCHHHH
RLCRTADAVYLGAVGGPKWDGMSQDLRPEKGLLRIRKELGLFANLRPAMLLPELAGACLL
HHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHH
RADIAARGLDLMVVRELTGDVYFGEPRGIEVRDGLRTGYNTMLYTEEEIRRIAVIAFETA
HHHHHHCCCHHEEEHHHCCCEEECCCCCCCHHCCHHCCCCCEEEEHHHHHHHHHHHHHHH
RKRRRRVCSVEKSNVLECSRLWKEVVMEVHREYADVELSHMYVDNAAMQLVRDPSQFDVI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCCCEEEE
LTGNIFGDILSDEASVITGSLGMLPSASTGAEGPGLFEPIHGSAPDIAGHDIANPLATIL
EECCHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SAAMMLRLGFDMAEEADHIETAVRKALADGFRTADIMEPGKELLGCAAMGDKVVERL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTICLLPGDGIGPEIVAQGVKVLEATAEKSGHQFVFETALIGGAAIDAAGDPLPEDTV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHCCCCCCCCCHHHH
RLCRTADAVYLGAVGGPKWDGMSQDLRPEKGLLRIRKELGLFANLRPAMLLPELAGACLL
HHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHH
RADIAARGLDLMVVRELTGDVYFGEPRGIEVRDGLRTGYNTMLYTEEEIRRIAVIAFETA
HHHHHHCCCHHEEEHHHCCCEEECCCCCCCHHCCHHCCCCCEEEEHHHHHHHHHHHHHHH
RKRRRRVCSVEKSNVLECSRLWKEVVMEVHREYADVELSHMYVDNAAMQLVRDPSQFDVI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCCCEEEE
LTGNIFGDILSDEASVITGSLGMLPSASTGAEGPGLFEPIHGSAPDIAGHDIANPLATIL
EECCHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SAAMMLRLGFDMAEEADHIETAVRKALADGFRTADIMEPGKELLGCAAMGDKVVERL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA