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The map label for this gene is trmFO [H]

Identifier: 220903788

GI number: 220903788

Start: 619494

End: 620861

Strand: Direct

Name: trmFO [H]

Synonym: Ddes_0511

Alternate gene names: 220903788

Gene position: 619494-620861 (Clockwise)

Preceding gene: 220903786

Following gene: 220903808

Centisome position: 21.56

GC content: 61.33

Gene sequence:

>1368_bases
ATGCAAAAATTGTCCATAGCCGTGGTGGGTGGTGGCCTTGCCGGCTGCGAATGCGCGTTGCGCCTAGCCCGCTCCGGACA
TACGGTGGAGCTTTTTGAACAAAAGCCCCTGTTCCGCTCACCAGCCCACGTCAGCGACCATCTGGCGGAGCTTGTATGCT
CCAACTCCCTGCGATCGGACGAACCAACCTCGGGCGTGGGGCTGCTCAAGGCTGAAATGCGCGCCTTGGGCAGCCAGTTT
ATGGAGGCTGCCGACGCCTGCCGCGTACCTGCTGGCAAGGCCCTTGCCGTAGACCGAGAGGCTTTTGCCCGCAGCATGAG
TGAACGTGTTGAGGCCGAAGCAAACATCCGTCTTGTGCGGCAGCAGGTGCAGTCGCTGGACGATGCGGCGCTGGAGCCGT
TCAAGGGAGAAGGGCGCGCTGTCATCGTGGCCGCAGGGCCGATGGCGTCCGACGGGCTGTCGGCTTCTCTGGCCGGAGCG
CTGGGCGAAAAGCACTGCTATTTTTACGATGCCATAGCTCCTATCATCTGGACGCATTCCTTGAATATGGACATTGTTTT
TCGTGCTTCGCGTTATGGGCAGGAAAATGGCGAGGCCGAAGGAGAGGGCGATTACCTGAACTGTCCCATGAGCCGCGAGG
AATACGAAGTTTTCTATCAGGCCCTGCTGGACGCCCAAAAGGTGGCCGCGCACGAATTTGAGCAGGAAAAACATTTTGAA
GGATGTATGCCGGTGGAAACACTGGCCGAGCGCGGTCACCGTACGCTCACCTTTGGCCCGCTCAAGCCTGTGGGCTTTGT
GGACCCGCGCACAGGCCGCCGCCCCTGGGCCATTCTGCAACTACGGGCTGAAAACGCCAACAGCGATACCTGCAACCTTG
TGGGCTGCCAGACCAAGCTGACCCAGGGGGAGCAGGCGCGTGTTTTCCGCCTTGTGCCGGGGCTGGAAAAGGCGGAATTT
GCCCGCTTCGGCAGCATGCACCGCAATACCTATGTGAACGCGCCGCATGTGCTGGATGCTGACCTGTCCCTCAAGGCGCG
GCCGGGCATTTATCTTGCCGGGCAGATTACCGGCGTGGAAGGCTATGTGGAATCCGCCGCCAGCGGCCTGTGGCTTGCCC
TCCTGCTGGATGCGCGCGCCCGTGGAATTGATCTGGAGCGCCCCCCGGCGGAAAGCGCCTTTGGGGCTTTGCTCGGGCAT
CTGCAAACCCCGGCCAAACGGTTTCAGCCTTCCAACGCCCACTTCGGCCTTATGCCGGACCTGGGGGAAAGGGCGCGCAA
AAAAGACCGCAAGGCGCTTTATTCGGCCCGGGCGCAAGAAGCATTCGCCCCCTGGCTGCATGAACTGAAGACAGCCGGTT
TGGCCTGA

Upstream 100 bases:

>100_bases
GGATAACAACTTCGATATAAAAAATAACATGTTATTGATTATCGCTCTCATACTTGCCAGAAGCCTTTTTTTCTTACACT
CTGCCCGCAGAGGAGAATAT

Downstream 100 bases:

>100_bases
GCCTGCCTTGTCTTTGCAGGAAGCGGCCAGCTCCCGGCAGGAATCGGTCCGAAGCACGTCTGAAGTCTGGCCCGGGCCGC
CAGTCCTGCCCGAAGTCAGC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQKLSIAVVGGGLAGCECALRLARSGHTVELFEQKPLFRSPAHVSDHLAELVCSNSLRSDEPTSGVGLLKAEMRALGSQF
MEAADACRVPAGKALAVDREAFARSMSERVEAEANIRLVRQQVQSLDDAALEPFKGEGRAVIVAAGPMASDGLSASLAGA
LGEKHCYFYDAIAPIIWTHSLNMDIVFRASRYGQENGEAEGEGDYLNCPMSREEYEVFYQALLDAQKVAAHEFEQEKHFE
GCMPVETLAERGHRTLTFGPLKPVGFVDPRTGRRPWAILQLRAENANSDTCNLVGCQTKLTQGEQARVFRLVPGLEKAEF
ARFGSMHRNTYVNAPHVLDADLSLKARPGIYLAGQITGVEGYVESAASGLWLALLLDARARGIDLERPPAESAFGALLGH
LQTPAKRFQPSNAHFGLMPDLGERARKKDRKALYSARAQEAFAPWLHELKTAGLA

Sequences:

>Translated_455_residues
MQKLSIAVVGGGLAGCECALRLARSGHTVELFEQKPLFRSPAHVSDHLAELVCSNSLRSDEPTSGVGLLKAEMRALGSQF
MEAADACRVPAGKALAVDREAFARSMSERVEAEANIRLVRQQVQSLDDAALEPFKGEGRAVIVAAGPMASDGLSASLAGA
LGEKHCYFYDAIAPIIWTHSLNMDIVFRASRYGQENGEAEGEGDYLNCPMSREEYEVFYQALLDAQKVAAHEFEQEKHFE
GCMPVETLAERGHRTLTFGPLKPVGFVDPRTGRRPWAILQLRAENANSDTCNLVGCQTKLTQGEQARVFRLVPGLEKAEF
ARFGSMHRNTYVNAPHVLDADLSLKARPGIYLAGQITGVEGYVESAASGLWLALLLDARARGIDLERPPAESAFGALLGH
LQTPAKRFQPSNAHFGLMPDLGERARKKDRKALYSARAQEAFAPWLHELKTAGLA
>Mature_455_residues
MQKLSIAVVGGGLAGCECALRLARSGHTVELFEQKPLFRSPAHVSDHLAELVCSNSLRSDEPTSGVGLLKAEMRALGSQF
MEAADACRVPAGKALAVDREAFARSMSERVEAEANIRLVRQQVQSLDDAALEPFKGEGRAVIVAAGPMASDGLSASLAGA
LGEKHCYFYDAIAPIIWTHSLNMDIVFRASRYGQENGEAEGEGDYLNCPMSREEYEVFYQALLDAQKVAAHEFEQEKHFE
GCMPVETLAERGHRTLTFGPLKPVGFVDPRTGRRPWAILQLRAENANSDTCNLVGCQTKLTQGEQARVFRLVPGLEKAEF
ARFGSMHRNTYVNAPHVLDADLSLKARPGIYLAGQITGVEGYVESAASGLWLALLLDARARGIDLERPPAESAFGALLGH
LQTPAKRFQPSNAHFGLMPDLGERARKKDRKALYSARAQEAFAPWLHELKTAGLA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49542; Mature: 49542

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLSIAVVGGGLAGCECALRLARSGHTVELFEQKPLFRSPAHVSDHLAELVCSNSLRSD
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
EPTSGVGLLKAEMRALGSQFMEAADACRVPAGKALAVDREAFARSMSERVEAEANIRLVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
QQVQSLDDAALEPFKGEGRAVIVAAGPMASDGLSASLAGALGEKHCYFYDAIAPIIWTHS
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHCC
LNMDIVFRASRYGQENGEAEGEGDYLNCPMSREEYEVFYQALLDAQKVAAHEFEQEKHFE
CCEEEEEEEHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GCMPVETLAERGHRTLTFGPLKPVGFVDPRTGRRPWAILQLRAENANSDTCNLVGCQTKL
CCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCHHC
TQGEQARVFRLVPGLEKAEFARFGSMHRNTYVNAPHVLDADLSLKARPGIYLAGQITGVE
CCCCCCEEEEECCCCCHHHHHHHCCHHCCCCCCCCCEECCCCCEECCCCEEEEEEEECHH
GYVESAASGLWLALLLDARARGIDLERPPAESAFGALLGHLQTPAKRFQPSNAHFGLMPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCC
LGERARKKDRKALYSARAQEAFAPWLHELKTAGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKLSIAVVGGGLAGCECALRLARSGHTVELFEQKPLFRSPAHVSDHLAELVCSNSLRSD
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
EPTSGVGLLKAEMRALGSQFMEAADACRVPAGKALAVDREAFARSMSERVEAEANIRLVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
QQVQSLDDAALEPFKGEGRAVIVAAGPMASDGLSASLAGALGEKHCYFYDAIAPIIWTHS
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHCC
LNMDIVFRASRYGQENGEAEGEGDYLNCPMSREEYEVFYQALLDAQKVAAHEFEQEKHFE
CCEEEEEEEHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GCMPVETLAERGHRTLTFGPLKPVGFVDPRTGRRPWAILQLRAENANSDTCNLVGCQTKL
CCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCHHC
TQGEQARVFRLVPGLEKAEFARFGSMHRNTYVNAPHVLDADLSLKARPGIYLAGQITGVE
CCCCCCEEEEECCCCCHHHHHHHCCHHCCCCCCCCCEECCCCCEECCCCEEEEEEEECHH
GYVESAASGLWLALLLDARARGIDLERPPAESAFGALLGHLQTPAKRFQPSNAHFGLMPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCC
LGERARKKDRKALYSARAQEAFAPWLHELKTAGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA