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The map label for this gene is leuD

Identifier: 220903784

GI number: 220903784

Start: 614856

End: 615353

Strand: Direct

Name: leuD

Synonym: Ddes_0507

Alternate gene names: 220903784

Gene position: 614856-615353 (Clockwise)

Preceding gene: 220903783

Following gene: 220903786

Centisome position: 21.4

GC content: 59.04

Gene sequence:

>498_bases
ATGAATTACAAGGGCAAGGCCCACAAGGTGGGCGAACATATTGACACGGACGCCATCATTCCCGCGCGCTTTCTTGTAAC
CACCGATGCACAGAAGCTCGGCGAAAACTGCATGTCCGGTCTTGAGCCAAGCTGGGCAAGCCGCGTGGCCCCCGGCGACA
TTATGGTGGCCGGGCGCAATTTCGGTTGCGGCTCATCGCGCGAGCATGCCCCCATCGCCATTCTGGGGGCGGGCATGCCC
GTGGTTATCGGCCACAGTTTTGCGCGCATTTTTTACCGCAACGCCTTCAATATGGGGCTGCTGCTTATGGAAGTGGGCGA
TGAGGTGGACAAGATCAGTGATGGAGATGCACTGGAAATTGACGCCTCCACAGGAGCAATCCGCAACCTGACCACCGGGG
CTGAAATCACCTGCCCGCCATTGCCTGCGTCAATGGCCGCCATTCTGGCAAAGGGAGGGCTGGTGGGCTACGTGAAGGAA
AGGCTCGCCCAGGTATAG

Upstream 100 bases:

>100_bases
GGCAGCGTCCCGAACGGGGGTGAACAGCAGGGCGTCACTGGCCGGAAGATGCAAGGAAATCTGCACAACCCATGCCGGGC
AGGCAATAAGGACCATAATC

Downstream 100 bases:

>100_bases
AGGCTTTTCATTTTTCTGCGTGTATTGTCGCTGAAGCAGCGTCATGTTGCCATTGCTCTGCGGCTGCGTGTAAAGGCGTC
TGCCACAAAGGCGCAGCGTC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNYKGKAHKVGEHIDTDAIIPARFLVTTDAQKLGENCMSGLEPSWASRVAPGDIMVAGRNFGCGSSREHAPIAILGAGMP
VVIGHSFARIFYRNAFNMGLLLMEVGDEVDKISDGDALEIDASTGAIRNLTTGAEITCPPLPASMAAILAKGGLVGYVKE
RLAQV

Sequences:

>Translated_165_residues
MNYKGKAHKVGEHIDTDAIIPARFLVTTDAQKLGENCMSGLEPSWASRVAPGDIMVAGRNFGCGSSREHAPIAILGAGMP
VVIGHSFARIFYRNAFNMGLLLMEVGDEVDKISDGDALEIDASTGAIRNLTTGAEITCPPLPASMAAILAKGGLVGYVKE
RLAQV
>Mature_165_residues
MNYKGKAHKVGEHIDTDAIIPARFLVTTDAQKLGENCMSGLEPSWASRVAPGDIMVAGRNFGCGSSREHAPIAILGAGMP
VVIGHSFARIFYRNAFNMGLLLMEVGDEVDKISDGDALEIDASTGAIRNLTTGAEITCPPLPASMAAILAKGGLVGYVKE
RLAQV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=114, Percent_Identity=40.3508771929825, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=172, Percent_Identity=33.1395348837209, Blast_Score=92, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=116, Percent_Identity=37.0689655172414, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_DESDA (B8J4G0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479096.1
- GeneID:   7284174
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0507
- HOGENOM:   HBG304838
- ProtClustDB:   PRK00439
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17369; Mature: 17369

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKGKAHKVGEHIDTDAIIPARFLVTTDAQKLGENCMSGLEPSWASRVAPGDIMVAGRN
CCCCCCCHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCEEEECCC
FGCGSSREHAPIAILGAGMPVVIGHSFARIFYRNAFNMGLLLMEVGDEVDKISDGDALEI
CCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEEEE
DASTGAIRNLTTGAEITCPPLPASMAAILAKGGLVGYVKERLAQV
ECCCCCHHHCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNYKGKAHKVGEHIDTDAIIPARFLVTTDAQKLGENCMSGLEPSWASRVAPGDIMVAGRN
CCCCCCCHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCEEEECCC
FGCGSSREHAPIAILGAGMPVVIGHSFARIFYRNAFNMGLLLMEVGDEVDKISDGDALEI
CCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCEEEE
DASTGAIRNLTTGAEITCPPLPASMAAILAKGGLVGYVKERLAQV
ECCCCCHHHCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA