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The map label for this gene is leuC

Identifier: 220903783

GI number: 220903783

Start: 613459

End: 614721

Strand: Direct

Name: leuC

Synonym: Ddes_0506

Alternate gene names: 220903783

Gene position: 613459-614721 (Clockwise)

Preceding gene: 220903782

Following gene: 220903784

Centisome position: 21.35

GC content: 60.25

Gene sequence:

>1263_bases
ATGGCTCAGACGCTTGCGCAAAAAATATTGCAAGCCCATACGGACGATGCGGTAGAACAGGACGGCCAGATCGTCCAGTG
CCGTGTATCCATGGTGCTTGCCAATGATATTACCGGGCCGCTGGCTATCAAGTCTTTTTACGGCATGGGCGCGAAAAAGG
TTTTTGACCGCACAAAGATTGCCCTGGTGATGGACCACTTTACCCCGCAAAAAGATATTGATTCGGCCAATCAGGTGCTC
ATCAGCCGCCGTTTTGCCGAAGAGCAAGACATTGTGCATTACTATGAAGGCGGTGACTGTGGTGTGGAACATACCCTGCT
GCCGGAACAGGGGCTGGTGGGGCCCGGTGATCTTGTTATCGGCGCTGACAGCCACACCTGCACCTATGGCGGCATCGGCG
CTTTTGCCACCGGCATGGGGTCCACAGACATCGCGGCGGGCATGGCCCTGGGCGAAACCTGGCTTAAGGTGCCGTCCACC
ATCCGCGTCAATATCAGCGGCGACATGCCCCGGTGGCTGCGGGGCAAGGATCTGATGCTCATGCTTATCGGCGCGATCGG
TGTGGACGGCGCCCTGTACAAGGCTCTGGAGTTCGGTGGCTCCGTGGTGGACGCTCTTTCCGTTGAGGGCCGCCTGAGCA
TGGCCAACATGGCTATCGAGGCCGGGGGCAAGGCCGGGCTTTTTGCCGTGGATGCCAAGACCCGCACCTATTGCGCCGAG
CACAAGCGCCCCGGCGTGCTGGAAAACATGGCCGCCGATTCCGGTGCGGTATATGAGCGCGTGGTGGACATGAATGTGAC
GGGCAAGGAGCCGGTGGTGGCCTGCCCGCATCTGCCGTCCAATGTGAAGCCTGTTTCCGAAGTACGCGATACCGCCATAC
AGCAGGTTGTTATCGGCTCCTGTACCAATGGCCGTATCAGCGACATGCGCGATGCCGCCGAGGTGCTGCGCGGCCGCAAG
GTGGATAAAGGCGTACGCTGTATCGTGTTGCCCTCCACGCCCACAGTGTGGAAGCAATGTCTCCGCGAAGGACTTATCGA
GGTATTTATGGAGGCGGGGTGCATTGTGGGACCCAGCACCTGCGGACCCTGTCTGGGCGGGCATATGGGCATTCTGGGCG
ATGGCGAGCGCGCTGTGGCTACCACCAACCGCAATTTCAGGGGGCGTATGGGCAGCCTGAGTTCCGAGGTTTACCTGGCA
AGCCCGCTGGTGGCGGCTGCCTCGGCTGTAACGGGGTATGTGGCCGGGCCGGACCAGTTATAA

Upstream 100 bases:

>100_bases
CCCTTTTTGAGTGGTATGTCCGCGGCAGGGGATTCATGCAACAGCGAATCCCGCCGGGCATTTTTCAAGGGGCGGTTCAA
GCTTCAAAGGAGAACAGATC

Downstream 100 bases:

>100_bases
AAACAGTGTGCTGCGGCCGTTCAGTCCGCCGTCGGGCAGCGTCCCGAACGGGGGTGAACAGCAGGGCGTCACTGGCCGGA
AGATGCAAGGAAATCTGCAC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKIALVMDHFTPQKDIDSANQVL
ISRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVIGADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPST
IRVNISGDMPRWLRGKDLMLMLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAE
HKRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGSCTNGRISDMRDAAEVLRGRK
VDKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPSTCGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLA
SPLVAAASAVTGYVAGPDQL

Sequences:

>Translated_420_residues
MAQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKIALVMDHFTPQKDIDSANQVL
ISRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVIGADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPST
IRVNISGDMPRWLRGKDLMLMLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAE
HKRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGSCTNGRISDMRDAAEVLRGRK
VDKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPSTCGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLA
SPLVAAASAVTGYVAGPDQL
>Mature_419_residues
AQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKIALVMDHFTPQKDIDSANQVLI
SRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVIGADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPSTI
RVNISGDMPRWLRGKDLMLMLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAEH
KRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGSCTNGRISDMRDAAEVLRGRKV
DKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPSTCGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLAS
PLVAAASAVTGYVAGPDQL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=444, Percent_Identity=27.9279279279279, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=32.6923076923077, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=25.0712250712251, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI2367097, Length=133, Percent_Identity=32.3308270676692, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787531, Length=358, Percent_Identity=24.0223463687151, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=445, Percent_Identity=27.4157303370787, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=344, Percent_Identity=28.4883720930233, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=281, Percent_Identity=27.0462633451957, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=376, Percent_Identity=23.4042553191489, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=33.3333333333333, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321429, Length=459, Percent_Identity=31.5904139433551, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323335, Length=446, Percent_Identity=30.2690582959641, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322261, Length=458, Percent_Identity=24.6724890829694, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI281365315, Length=451, Percent_Identity=27.0509977827051, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17864292, Length=451, Percent_Identity=27.0509977827051, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571643, Length=444, Percent_Identity=26.8018018018018, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161076999, Length=335, Percent_Identity=28.6567164179104, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_DESDA (B8J4F9)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479095.1
- GeneID:   7284173
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0506
- HOGENOM:   HBG330745
- ProtClustDB:   PRK00402
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 44418; Mature: 44286

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKI
CHHHHHHHHHHHCCCHHHHCCCCEEEEEEEEHHHCCCCCHHHHHHHHCCCHHHHHHHHHE
ALVMDHFTPQKDIDSANQVLISRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVI
EEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
GADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPSTIRVNISGDMPRWLRGKDLML
ECCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHH
MLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAE
HHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHEECCCCCCEEEECCCHHHHHHH
HKRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGS
CCCCCHHHHHCCCCCHHHHHEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC
CTNGRISDMRDAAEVLRGRKVDKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPST
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEECCCC
CGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLASPLVAAASAVTGYVAGPDQL
CCHHHCCCCCEECCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKI
HHHHHHHHHHHCCCHHHHCCCCEEEEEEEEHHHCCCCCHHHHHHHHCCCHHHHHHHHHE
ALVMDHFTPQKDIDSANQVLISRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVI
EEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
GADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPSTIRVNISGDMPRWLRGKDLML
ECCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHH
MLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAE
HHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHEECCCCCCEEEECCCHHHHHHH
HKRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGS
CCCCCHHHHHCCCCCHHHHHEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC
CTNGRISDMRDAAEVLRGRKVDKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPST
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEECCCC
CGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLASPLVAAASAVTGYVAGPDQL
CCHHHCCCCCEECCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA