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The map label for this gene is pgk

Identifier: 220903757

GI number: 220903757

Start: 587517

End: 588686

Strand: Reverse

Name: pgk

Synonym: Ddes_0479

Alternate gene names: 220903757

Gene position: 588686-587517 (Counterclockwise)

Preceding gene: 220903758

Following gene: 220903756

Centisome position: 20.49

GC content: 60.34

Gene sequence:

>1170_bases
ATGCCCGTAACAAAAATGCAGGATCTTGATCTTGGCGGAAAAACGGTTGTCATCCGCGAAGACCTCAATGTGCCGATGAA
GGACGGAATCATCACCAATGACAAGCGCATCCGCGCTGCCCTGCCCACAATACAGCTGGCGCTGGAAAAAGGCGCGGGCG
TCATTGTGCTGTCGCACCTGGGGCGCCCCACTGAAGGGCAGTATGACCAGCAGTTTTCTCTCGCTCCCGTGGCCGACCGT
CTGGCCCAGCTGCTGGGGCAGCCCGTGACCCTGGCAAAGAGCCTGGACGAGGCCAAAACTGCTCCCGGACAGGTAACCCT
GCTCGAAAACGTGCGCTTTCTGCCCGGAGAAAAAAAGAATGATCCCGAACTGGCAGCCAAACTTGCCGGGCTTGGCGATG
TGTATGTAATGGACGCTTTCGGCTCGGCCCACCGCGCCCACGCTTCTACCGAAGGCGCTGTGCGCACCGCCGCTGTGGCC
TGCGCCGGTCCCCTGCTGCAGGCCGAACTGGAAGCCTTTGACAAGGTGCTTGACAATCCCGCCAGGCCTGTGGTGGCCAT
TGTCGGCGGCGCCAAGGTTTCCACCAAGCTGACCCTGCTCGAAAACCTGCTGGAAAAAGTGGATGTGCTCATCGTGGGCG
GCGGCATCGCCAACACCTTTCTTGCCGCGGCCGGATATATGGTGGGCAAGTCTCTGTATGAAGAAGACCTGCTGCCCGAA
GCCCAGAAAATCATGGCGCTGGCAAAGACCCTCAACAAGGAACTGCCCCTGCCCGTGGACGTGATCACCGCCGAAGAACT
GGCCCCCCGCCAGCAGACCATGCTGCACGCCGTGGGTGATGTGCCGGGCGACCAGATGATTCTTGATATCGGCCCCGAAA
CGCTGACCCTGTATGAAAAATTTCTCTCCAAGGCGGCCACGGTGGTCTGGAACGGCCCGGTCGGCGCATTTGAGATAGAA
CCTTTTGGCGACGGGACAAAGGCCCTGGCCGAATACCTGAGCGATTCCAAGGCCTTTGTGGTTGTGGGTGGCGGCGACAG
CGTAGCTGCCGTGGAAAAATACGGCCTGGCCGACCGCATGGGCTATATCTCTACCGGCGGCGGCGCTTCGCTGGAACTGC
TTGAAGGCAAAAAACTGCCTTCCGTTGCGGCGCTGGAAGACCGCGCCTAG

Upstream 100 bases:

>100_bases
GCGCATACTTCACCGGTGGCATGCTCTGGCTTGCAAGGCCCGCCCGGGCATTTGCTTTCACTGAAAATCACGCTGCAAGT
CCAACAAAGAGGAGAGCGCC

Downstream 100 bases:

>100_bases
GCGGACGCATGAAAAAACGCTTCATCCTGAATTCGCTGCTTCTGCTGCCGCTGTGGATCTGCTGCCTGCTGGCAGCGCCC
GCCGCCCAGGCCCAGGAACT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPVTKMQDLDLGGKTVVIREDLNVPMKDGIITNDKRIRAALPTIQLALEKGAGVIVLSHLGRPTEGQYDQQFSLAPVADR
LAQLLGQPVTLAKSLDEAKTAPGQVTLLENVRFLPGEKKNDPELAAKLAGLGDVYVMDAFGSAHRAHASTEGAVRTAAVA
CAGPLLQAELEAFDKVLDNPARPVVAIVGGAKVSTKLTLLENLLEKVDVLIVGGGIANTFLAAAGYMVGKSLYEEDLLPE
AQKIMALAKTLNKELPLPVDVITAEELAPRQQTMLHAVGDVPGDQMILDIGPETLTLYEKFLSKAATVVWNGPVGAFEIE
PFGDGTKALAEYLSDSKAFVVVGGGDSVAAVEKYGLADRMGYISTGGGASLELLEGKKLPSVAALEDRA

Sequences:

>Translated_389_residues
MPVTKMQDLDLGGKTVVIREDLNVPMKDGIITNDKRIRAALPTIQLALEKGAGVIVLSHLGRPTEGQYDQQFSLAPVADR
LAQLLGQPVTLAKSLDEAKTAPGQVTLLENVRFLPGEKKNDPELAAKLAGLGDVYVMDAFGSAHRAHASTEGAVRTAAVA
CAGPLLQAELEAFDKVLDNPARPVVAIVGGAKVSTKLTLLENLLEKVDVLIVGGGIANTFLAAAGYMVGKSLYEEDLLPE
AQKIMALAKTLNKELPLPVDVITAEELAPRQQTMLHAVGDVPGDQMILDIGPETLTLYEKFLSKAATVVWNGPVGAFEIE
PFGDGTKALAEYLSDSKAFVVVGGGDSVAAVEKYGLADRMGYISTGGGASLELLEGKKLPSVAALEDRA
>Mature_388_residues
PVTKMQDLDLGGKTVVIREDLNVPMKDGIITNDKRIRAALPTIQLALEKGAGVIVLSHLGRPTEGQYDQQFSLAPVADRL
AQLLGQPVTLAKSLDEAKTAPGQVTLLENVRFLPGEKKNDPELAAKLAGLGDVYVMDAFGSAHRAHASTEGAVRTAAVAC
AGPLLQAELEAFDKVLDNPARPVVAIVGGAKVSTKLTLLENLLEKVDVLIVGGGIANTFLAAAGYMVGKSLYEEDLLPEA
QKIMALAKTLNKELPLPVDVITAEELAPRQQTMLHAVGDVPGDQMILDIGPETLTLYEKFLSKAATVVWNGPVGAFEIEP
FGDGTKALAEYLSDSKAFVVVGGGDSVAAVEKYGLADRMGYISTGGGASLELLEGKKLPSVAALEDRA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=42.0924574209246, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=41.7073170731707, Blast_Score=289, Evalue=4e-78,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=56.6666666666667, Blast_Score=423, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17508823, Length=411, Percent_Identity=44.2822384428224, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=43.2038834951456, Blast_Score=290, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=43.2367149758454, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=43.3823529411765, Blast_Score=291, Evalue=6e-79,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_DESDA (B8J4D3)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479069.1
- GeneID:   7284144
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0479
- HOGENOM:   HBG453500
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40941; Mature: 40810

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 112-112 BINDING 145-145 BINDING 196-196 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTKMQDLDLGGKTVVIREDLNVPMKDGIITNDKRIRAALPTIQLALEKGAGVIVLSHL
CCCCCCCCCCCCCCEEEEECCCCCCHHCCEEECCCHHHHHCCHHHHHHHCCCCEEEEECC
GRPTEGQYDQQFSLAPVADRLAQLLGQPVTLAKSLDEAKTAPGQVTLLENVRFLPGEKKN
CCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCCC
DPELAAKLAGLGDVYVMDAFGSAHRAHASTEGAVRTAAVACAGPLLQAELEAFDKVLDNP
CHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARPVVAIVGGAKVSTKLTLLENLLEKVDVLIVGGGIANTFLAAAGYMVGKSLYEEDLLPE
CCCEEEEECCCCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
AQKIMALAKTLNKELPLPVDVITAEELAPRQQTMLHAVGDVPGDQMILDIGPETLTLYEK
HHHHHHHHHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
FLSKAATVVWNGPVGAFEIEPFGDGTKALAEYLSDSKAFVVVGGGDSVAAVEKYGLADRM
HHHHHEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHC
GYISTGGGASLELLEGKKLPSVAALEDRA
CEEECCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVTKMQDLDLGGKTVVIREDLNVPMKDGIITNDKRIRAALPTIQLALEKGAGVIVLSHL
CCCCCCCCCCCCCEEEEECCCCCCHHCCEEECCCHHHHHCCHHHHHHHCCCCEEEEECC
GRPTEGQYDQQFSLAPVADRLAQLLGQPVTLAKSLDEAKTAPGQVTLLENVRFLPGEKKN
CCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCCC
DPELAAKLAGLGDVYVMDAFGSAHRAHASTEGAVRTAAVACAGPLLQAELEAFDKVLDNP
CHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARPVVAIVGGAKVSTKLTLLENLLEKVDVLIVGGGIANTFLAAAGYMVGKSLYEEDLLPE
CCCEEEEECCCCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHH
AQKIMALAKTLNKELPLPVDVITAEELAPRQQTMLHAVGDVPGDQMILDIGPETLTLYEK
HHHHHHHHHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
FLSKAATVVWNGPVGAFEIEPFGDGTKALAEYLSDSKAFVVVGGGDSVAAVEKYGLADRM
HHHHHEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHC
GYISTGGGASLELLEGKKLPSVAALEDRA
CEEECCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA