Click here to switch to the map view.

The map label for this gene is mipA [H]

Identifier: 220903756

GI number: 220903756

Start: 586690

End: 587508

Strand: Reverse

Name: mipA [H]

Synonym: Ddes_0478

Alternate gene names: 220903756

Gene position: 587508-586690 (Counterclockwise)

Preceding gene: 220903757

Following gene: 220903755

Centisome position: 20.45

GC content: 57.39

Gene sequence:

>819_bases
ATGAAAAAACGCTTCATCCTGAATTCGCTGCTTCTGCTGCCGCTGTGGATCTGCTGCCTGCTGGCAGCGCCCGCCGCCCA
GGCCCAGGAACTGTGGGAAGATGATCCGGACGCCCCTGCCGTAACCGCGCAGCAGGACCGGTGGGGTTTTAATGCGGGCA
TCGGGGCATCGTTGCGCACATCGGAATACAAGGGCATCAACTCGCTGGGTTCGCCTCTGCCCCTGCTGGGCTACGAAGGC
AACTGGCTGTACCTGCGTGGCCTGAGCGGCGGTGTGCACGTCTTCAAGAACCGCTATCACGAGTTCAATGTCCAGCTTTC
CTATCTGCCACAGCATTTTTATGCGTCCTGGAGCGACAATGACAGGATGAAACGGCTGGATGACCGCTATTCTTCGCTCA
TGGCCGGTCTGAACTACACCCTGCGCTCCCGCTATGGCCTGGCTTCGGCCACACTGTCCACGGACGTTCTCGGCGTCAAT
AACGGCGTCATGGCCGATGTTTCCTATGCCTATCCACTGCGCTTCGAAAATATTGTCATCATGCCCTCGGTGGGTGCGCA
GTGGACTGACAGCAACTACAATGACTATTATTACAGTGTCAGTTCCAGTGAGTCGCGCAAAAGCGGCCTGAAAGAATACA
GCCCGGAAAGCGCGTTCTCACCCTATGCCGGGCTGACGCTGCGCGTGGCGCTTACCGACAGCTGGAGCGTCCTTGTCAAC
GGCAAGGCCCTTTTTCTGGGAAGCGAAGTGACCGACAGCCCCATGGTGGAACGCAGTACCACGTACTCTTTCAATGCGGG
TTTTCTGTACGCTTTTTAG

Upstream 100 bases:

>100_bases
TGGGCTATATCTCTACCGGCGGCGGCGCTTCGCTGGAACTGCTTGAAGGCAAAAAACTGCCTTCCGTTGCGGCGCTGGAA
GACCGCGCCTAGGCGGACGC

Downstream 100 bases:

>100_bases
AGCAATCTGCATGTGAGAGGGTTGCCTGCAGCCAAAGGCCAAAGGCCGTGATCTTTTTTGGGATCACGGCCTTTTTGTTT
GCTTTCAGACCGGCACAACC

Product: MltA-interacting MipA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKRFILNSLLLLPLWICCLLAAPAAQAQELWEDDPDAPAVTAQQDRWGFNAGIGASLRTSEYKGINSLGSPLPLLGYEG
NWLYLRGLSGGVHVFKNRYHEFNVQLSYLPQHFYASWSDNDRMKRLDDRYSSLMAGLNYTLRSRYGLASATLSTDVLGVN
NGVMADVSYAYPLRFENIVIMPSVGAQWTDSNYNDYYYSVSSSESRKSGLKEYSPESAFSPYAGLTLRVALTDSWSVLVN
GKALFLGSEVTDSPMVERSTTYSFNAGFLYAF

Sequences:

>Translated_272_residues
MKKRFILNSLLLLPLWICCLLAAPAAQAQELWEDDPDAPAVTAQQDRWGFNAGIGASLRTSEYKGINSLGSPLPLLGYEG
NWLYLRGLSGGVHVFKNRYHEFNVQLSYLPQHFYASWSDNDRMKRLDDRYSSLMAGLNYTLRSRYGLASATLSTDVLGVN
NGVMADVSYAYPLRFENIVIMPSVGAQWTDSNYNDYYYSVSSSESRKSGLKEYSPESAFSPYAGLTLRVALTDSWSVLVN
GKALFLGSEVTDSPMVERSTTYSFNAGFLYAF
>Mature_272_residues
MKKRFILNSLLLLPLWICCLLAAPAAQAQELWEDDPDAPAVTAQQDRWGFNAGIGASLRTSEYKGINSLGSPLPLLGYEG
NWLYLRGLSGGVHVFKNRYHEFNVQLSYLPQHFYASWSDNDRMKRLDDRYSSLMAGLNYTLRSRYGLASATLSTDVLGVN
NGVMADVSYAYPLRFENIVIMPSVGAQWTDSNYNDYYYSVSSSESRKSGLKEYSPESAFSPYAGLTLRVALTDSWSVLVN
GKALFLGSEVTDSPMVERSTTYSFNAGFLYAF

Specific function: May serve as a scaffold protein required for the formation of a complex with mrcB/ponB and mltA, this complex could play a role in enlargement and septation of the murein sacculus [H]

COG id: COG3713

COG function: function code M; Outer membrane protein V

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mipA/ompV family [H]

Homologues:

Organism=Escherichia coli, GI1788082, Length=223, Percent_Identity=35.4260089686099, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1790010, Length=220, Percent_Identity=32.2727272727273, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010583 [H]

Pfam domain/function: PF06629 MipA [H]

EC number: NA

Molecular weight: Translated: 30363; Mature: 30363

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRFILNSLLLLPLWICCLLAAPAAQAQELWEDDPDAPAVTAQQDRWGFNAGIGASLRT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEEC
SEYKGINSLGSPLPLLGYEGNWLYLRGLSGGVHVFKNRYHEFNVQLSYLPQHFYASWSDN
CCCCCCHHCCCCCCEEECCCCEEEEEECCCCHHHHHCCCEEEEEEEEECCCEEEECCCCC
DRMKRLDDRYSSLMAGLNYTLRSRYGLASATLSTDVLGVNNGVMADVSYAYPLRFENIVI
CHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCEEEEECCCCEEEEEEEECCEEECCEEE
MPSVGAQWTDSNYNDYYYSVSSSESRKSGLKEYSPESAFSPYAGLTLRVALTDSWSVLVN
ECCCCCCCCCCCCCCEEEEECCCCHHHHCHHHCCCCCCCCCCCCEEEEEEEECCEEEEEC
GKALFLGSEVTDSPMVERSTTYSFNAGFLYAF
CEEEEEECCCCCCCCCCCCCEEEECCCEEEEC
>Mature Secondary Structure
MKKRFILNSLLLLPLWICCLLAAPAAQAQELWEDDPDAPAVTAQQDRWGFNAGIGASLRT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEEC
SEYKGINSLGSPLPLLGYEGNWLYLRGLSGGVHVFKNRYHEFNVQLSYLPQHFYASWSDN
CCCCCCHHCCCCCCEEECCCCEEEEEECCCCHHHHHCCCEEEEEEEEECCCEEEECCCCC
DRMKRLDDRYSSLMAGLNYTLRSRYGLASATLSTDVLGVNNGVMADVSYAYPLRFENIVI
CHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCEEEEECCCCEEEEEEEECCEEECCEEE
MPSVGAQWTDSNYNDYYYSVSSSESRKSGLKEYSPESAFSPYAGLTLRVALTDSWSVLVN
ECCCCCCCCCCCCCCEEEEECCCCHHHHCHHHCCCCCCCCCCCCEEEEEEEECCEEEEEC
GKALFLGSEVTDSPMVERSTTYSFNAGFLYAF
CEEEEEECCCCCCCCCCCCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10037771; 10806384 [H]