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The map label for this gene is gmd [H]

Identifier: 220903690

GI number: 220903690

Start: 501995

End: 503143

Strand: Reverse

Name: gmd [H]

Synonym: Ddes_0412

Alternate gene names: 220903690

Gene position: 503143-501995 (Counterclockwise)

Preceding gene: 220903691

Following gene: 220903689

Centisome position: 17.51

GC content: 56.83

Gene sequence:

>1149_bases
ATGAAGAAAGCGCTCATAACGGGCATCACCGGTCAGGACGGGGCCTATCTTGCAGAATTTCTGTTACGCAAAGGCTATGA
GGTGCACGGCATCAAGCGCCGCGCCTCGCTCTTCAATACAGACCGCATTGACCATCTCTATCAGGATCCGCACACCAGTG
CGCGGCATTTTATACTGCACTACGGCGATCTGAGTGATTCCAGCAATCTCATCCGCATCATGCAGGAAGTGCAGCCCGAC
GAAGTATACAATCTGGCAGCCCAAAGCCATGTGCAGGTGTCCTTTGAGTCCCCGGAATACACGGCGGACGTAGATGCTCT
TGGTGCACTGCGCCTGCTGGAGGCCATCCGCATTGCCGGGCTGACCGCGAACACCAGATTTTACCAGGCGTCCACCTCCG
AACTTTTCGGCCTGGTGCAGGAAGTGCCGCAGACCGAAAAAACGCCCTTCTACCCCCGTTCGCCTTACGCCTGCGCCAAA
CTCTATGCTTACTGGATCACGGTCAATTACCGCGAAGCCTACGGCATGTACGCTTGCAACGGCATTTTGTTCAATCATGA
ATCTCCCATTCGCGGCGAAACCTTTGTGACCCGCAAAATCACCCGCGCGCTTTCCCGCATGGTTCTGGGCCTTCAGGATT
GCCTCTACCTCGGCAATATGGATGCCAAGCGCGACTGGGGACACGCCAGGGACTATGTTGAAATGCAGTGGCTTATGCTG
CAGCAGGAACAAGCGGACGACTTTGTCATTGCCACAGGGCGACAATTCTCGGTACGCGATTTCGTCAACACGGCAGCGGC
TGAAGTGGGTTTAACGCTTGCATGGCAAGGCCAAGGCGTGGACGAGACCGGCACAGTGGAAGCTGTGGACCTGAATAAAC
TGCAGCAGGCTGCTGGCAACCGTCAGGGGCTGGAGTGCCACGTCAAGCCCGGCGATGTCATTGTGCGGGTAGACCCGCGC
TACTTCCGCCCCACCGAGGTGGAAACCTTGCTGGGCAATCCGGCCAAGGCCAAGGAAAAGCTGGGTTGGGAGCCGAGCAC
ATCCTTTGAGGATATGGTGGCCGAAATGGCGCGCGAAGATCTAGCCCTGAGCATGCGTGATGCCGTGTGCAAGGTGGCAG
GATTCAAGACATTCAGCCATAACGAGTAG

Upstream 100 bases:

>100_bases
TGGACATCGTCCACCATGCAATATAGAATTATAAGTTCACCTTGAGCAATCTGAACGTAAAAAATACTATGACAATTTGT
CAGGTCAGGAAATAAGGATC

Downstream 100 bases:

>100_bases
CCCGCATGGACAAAGATTCGCTTATTTATGTAGCAGGCCACCGCGGGCTTGCGGGCAGTGCCATTTGTCGCGCTCTTGCC
CGTGATGGCTATGAAAATCT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKKALITGITGQDGAYLAEFLLRKGYEVHGIKRRASLFNTDRIDHLYQDPHTSARHFILHYGDLSDSSNLIRIMQEVQPD
EVYNLAAQSHVQVSFESPEYTADVDALGALRLLEAIRIAGLTANTRFYQASTSELFGLVQEVPQTEKTPFYPRSPYACAK
LYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALSRMVLGLQDCLYLGNMDAKRDWGHARDYVEMQWLML
QQEQADDFVIATGRQFSVRDFVNTAAAEVGLTLAWQGQGVDETGTVEAVDLNKLQQAAGNRQGLECHVKPGDVIVRVDPR
YFRPTEVETLLGNPAKAKEKLGWEPSTSFEDMVAEMAREDLALSMRDAVCKVAGFKTFSHNE

Sequences:

>Translated_382_residues
MKKALITGITGQDGAYLAEFLLRKGYEVHGIKRRASLFNTDRIDHLYQDPHTSARHFILHYGDLSDSSNLIRIMQEVQPD
EVYNLAAQSHVQVSFESPEYTADVDALGALRLLEAIRIAGLTANTRFYQASTSELFGLVQEVPQTEKTPFYPRSPYACAK
LYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALSRMVLGLQDCLYLGNMDAKRDWGHARDYVEMQWLML
QQEQADDFVIATGRQFSVRDFVNTAAAEVGLTLAWQGQGVDETGTVEAVDLNKLQQAAGNRQGLECHVKPGDVIVRVDPR
YFRPTEVETLLGNPAKAKEKLGWEPSTSFEDMVAEMAREDLALSMRDAVCKVAGFKTFSHNE
>Mature_382_residues
MKKALITGITGQDGAYLAEFLLRKGYEVHGIKRRASLFNTDRIDHLYQDPHTSARHFILHYGDLSDSSNLIRIMQEVQPD
EVYNLAAQSHVQVSFESPEYTADVDALGALRLLEAIRIAGLTANTRFYQASTSELFGLVQEVPQTEKTPFYPRSPYACAK
LYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALSRMVLGLQDCLYLGNMDAKRDWGHARDYVEMQWLML
QQEQADDFVIATGRQFSVRDFVNTAAAEVGLTLAWQGQGVDETGTVEAVDLNKLQQAAGNRQGLECHVKPGDVIVRVDPR
YFRPTEVETLLGNPAKAKEKLGWEPSTSFEDMVAEMAREDLALSMRDAVCKVAGFKTFSHNE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=361, Percent_Identity=56.5096952908587, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1788366, Length=374, Percent_Identity=70.0534759358289, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI48994969, Length=363, Percent_Identity=25.068870523416, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788353, Length=265, Percent_Identity=25.2830188679245, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=370, Percent_Identity=60.5405405405405, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539424, Length=364, Percent_Identity=60.4395604395604, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=364, Percent_Identity=60.4395604395604, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=364, Percent_Identity=60.4395604395604, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=364, Percent_Identity=60.4395604395604, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901788, Length=364, Percent_Identity=60.4395604395604, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=371, Percent_Identity=56.3342318059299, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43071; Mature: 43071

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGAYLAEFLLRKGYEVHGIKRRASLFNTDRIDHLYQDPHTSARHFILH
CCCCEEECCCCCCHHHHHHHHHHCCCCEECHHHHHHHHCCHHHHHHHCCCCCCCEEEEEE
YGDLSDSSNLIRIMQEVQPDEVYNLAAQSHVQVSFESPEYTADVDALGALRLLEAIRIAG
ECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
LTANTRFYQASTSELFGLVQEVPQTEKTPFYPRSPYACAKLYAYWITVNYREAYGMYACN
CCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEEC
GILFNHESPIRGETFVTRKITRALSRMVLGLQDCLYLGNMDAKRDWGHARDYVEMQWLML
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QQEQADDFVIATGRQFSVRDFVNTAAAEVGLTLAWQGQGVDETGTVEAVDLNKLQQAAGN
HHCCCCCEEEECCCCEEHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEECHHHHHHHHCC
RQGLECHVKPGDVIVRVDPRYFRPTEVETLLGNPAKAKEKLGWEPSTSFEDMVAEMARED
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
LALSMRDAVCKVAGFKTFSHNE
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKALITGITGQDGAYLAEFLLRKGYEVHGIKRRASLFNTDRIDHLYQDPHTSARHFILH
CCCCEEECCCCCCHHHHHHHHHHCCCCEECHHHHHHHHCCHHHHHHHCCCCCCCEEEEEE
YGDLSDSSNLIRIMQEVQPDEVYNLAAQSHVQVSFESPEYTADVDALGALRLLEAIRIAG
ECCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
LTANTRFYQASTSELFGLVQEVPQTEKTPFYPRSPYACAKLYAYWITVNYREAYGMYACN
CCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEEC
GILFNHESPIRGETFVTRKITRALSRMVLGLQDCLYLGNMDAKRDWGHARDYVEMQWLML
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QQEQADDFVIATGRQFSVRDFVNTAAAEVGLTLAWQGQGVDETGTVEAVDLNKLQQAAGN
HHCCCCCEEEECCCCEEHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEECHHHHHHHHCC
RQGLECHVKPGDVIVRVDPRYFRPTEVETLLGNPAKAKEKLGWEPSTSFEDMVAEMARED
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
LALSMRDAVCKVAGFKTFSHNE
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]