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The map label for this gene is fcl [C]

Identifier: 220903689

GI number: 220903689

Start: 501045

End: 501989

Strand: Reverse

Name: fcl [C]

Synonym: Ddes_0411

Alternate gene names: 220903689

Gene position: 501989-501045 (Counterclockwise)

Preceding gene: 220903690

Following gene: 220903688

Centisome position: 17.47

GC content: 56.19

Gene sequence:

>945_bases
ATGGACAAAGATTCGCTTATTTATGTAGCAGGCCACCGCGGGCTTGCGGGCAGTGCCATTTGTCGCGCTCTTGCCCGTGA
TGGCTATGAAAATCTGCTGACGCGCACGCACGCAGAACTGGATCTGTGTGATCAGGCGGCAGTGCGAACCTTTTTTGCGC
AGTACCGGCCTGCCATCGTGGTACTTGCCGCCGCCAAGGTGGGCGGCATTCATGCCAACGCCACATATCCAGCAGAATTT
ATCTACCAGAACTTGCAGATACAGAACAACGTCATAGACAGCGCCTACCGCAACGACTGCAAAAAACTGCTGTTTCTGGG
GTCATCGTGCATATACCCCAAGATGTGCCCACAGCCCATCAAGGAAGAGTATCTGCTCACAGGGCCGCTGGAACCCACCA
ATGATGCCTACGCCCTGGCGAAAATAGCAGGTATCAAGATGTGCCAGGCCTACCGCAAGCAGTATGGCTTTGACGCCATC
AGCGCCATGCCAACCAATCTGTATGGCCCGGGCGATAATTACCACCCGGAAAACAGCCATGTGATCCCGGCGCTTATCCG
CCGTTTTCATGAAGCCAAAATGGCCGGAGCCGAAAAGGTGACCATATGGGGAACGGGCAACGCCCTGCGCGAATTTCTCT
ATGTGGATGACATGGCCGAAGCCTGTATTTTTCTGCTGAAAAACTACTCGGATTTTGAACACGTCAACGCGGGCTGCGGC
TCCGACATCAGCATCATCGACACCGCCCGGCTTATTGCCCGTGTGGTAGGCTTTGAGGGCAGCATTGACACCGACCCCAC
AAAGCCCGATGGTACGCCGCGCAAACTTATGGCCTCGGGCAAGCTCTTCGGCATGGGCTGGAAGCCCAGGGTGGAGTTTG
AGGAAGGCCTACGGGCAACATACAGGGACTTCTGCGCCCGTAATGGGGCGGGGGCCGCATGCTGA

Upstream 100 bases:

>100_bases
TGGTGGCCGAAATGGCGCGCGAAGATCTAGCCCTGAGCATGCGTGATGCCGTGTGCAAGGTGGCAGGATTCAAGACATTC
AGCCATAACGAGTAGCCCGC

Downstream 100 bases:

>100_bases
ATCCGCAGCTTAGCCAGATTTTTCGAGCTATTTACACCCGGTTGAGCAAAAACCTGCAAAGCCGCTTCTGGAGGGTGATG
ATTTTTTCCGTACTGGTCGC

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDKDSLIYVAGHRGLAGSAICRALARDGYENLLTRTHAELDLCDQAAVRTFFAQYRPAIVVLAAAKVGGIHANATYPAEF
IYQNLQIQNNVIDSAYRNDCKKLLFLGSSCIYPKMCPQPIKEEYLLTGPLEPTNDAYALAKIAGIKMCQAYRKQYGFDAI
SAMPTNLYGPGDNYHPENSHVIPALIRRFHEAKMAGAEKVTIWGTGNALREFLYVDDMAEACIFLLKNYSDFEHVNAGCG
SDISIIDTARLIARVVGFEGSIDTDPTKPDGTPRKLMASGKLFGMGWKPRVEFEEGLRATYRDFCARNGAGAAC

Sequences:

>Translated_314_residues
MDKDSLIYVAGHRGLAGSAICRALARDGYENLLTRTHAELDLCDQAAVRTFFAQYRPAIVVLAAAKVGGIHANATYPAEF
IYQNLQIQNNVIDSAYRNDCKKLLFLGSSCIYPKMCPQPIKEEYLLTGPLEPTNDAYALAKIAGIKMCQAYRKQYGFDAI
SAMPTNLYGPGDNYHPENSHVIPALIRRFHEAKMAGAEKVTIWGTGNALREFLYVDDMAEACIFLLKNYSDFEHVNAGCG
SDISIIDTARLIARVVGFEGSIDTDPTKPDGTPRKLMASGKLFGMGWKPRVEFEEGLRATYRDFCARNGAGAAC
>Mature_314_residues
MDKDSLIYVAGHRGLAGSAICRALARDGYENLLTRTHAELDLCDQAAVRTFFAQYRPAIVVLAAAKVGGIHANATYPAEF
IYQNLQIQNNVIDSAYRNDCKKLLFLGSSCIYPKMCPQPIKEEYLLTGPLEPTNDAYALAKIAGIKMCQAYRKQYGFDAI
SAMPTNLYGPGDNYHPENSHVIPALIRRFHEAKMAGAEKVTIWGTGNALREFLYVDDMAEACIFLLKNYSDFEHVNAGCG
SDISIIDTARLIARVVGFEGSIDTDPTKPDGTPRKLMASGKLFGMGWKPRVEFEEGLRATYRDFCARNGAGAAC

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=282, Percent_Identity=34.7517730496454, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI7657641, Length=275, Percent_Identity=27.6363636363636, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=25.9818731117825, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788365, Length=308, Percent_Identity=53.8961038961039, Blast_Score=344, Evalue=4e-96,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=26.9005847953216, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994969, Length=296, Percent_Identity=24.6621621621622, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554428, Length=285, Percent_Identity=30.1754385964912, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532424, Length=277, Percent_Identity=26.7148014440433, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922778, Length=286, Percent_Identity=33.5664335664336, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34544; Mature: 34544

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKDSLIYVAGHRGLAGSAICRALARDGYENLLTRTHAELDLCDQAAVRTFFAQYRPAIV
CCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VLAAAKVGGIHANATYPAEFIYQNLQIQNNVIDSAYRNDCKKLLFLGSSCIYPKMCPQPI
EEEEHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEEYLLTGPLEPTNDAYALAKIAGIKMCQAYRKQYGFDAISAMPTNLYGPGDNYHPENSH
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCC
VIPALIRRFHEAKMAGAEKVTIWGTGNALREFLYVDDMAEACIFLLKNYSDFEHVNAGCG
CHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
SDISIIDTARLIARVVGFEGSIDTDPTKPDGTPRKLMASGKLFGMGWKPRVEFEEGLRAT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCHHHHHHHH
YRDFCARNGAGAAC
HHHHHHCCCCCCCC
>Mature Secondary Structure
MDKDSLIYVAGHRGLAGSAICRALARDGYENLLTRTHAELDLCDQAAVRTFFAQYRPAIV
CCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VLAAAKVGGIHANATYPAEFIYQNLQIQNNVIDSAYRNDCKKLLFLGSSCIYPKMCPQPI
EEEEHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEEYLLTGPLEPTNDAYALAKIAGIKMCQAYRKQYGFDAISAMPTNLYGPGDNYHPENSH
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCC
VIPALIRRFHEAKMAGAEKVTIWGTGNALREFLYVDDMAEACIFLLKNYSDFEHVNAGCG
CHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
SDISIIDTARLIARVVGFEGSIDTDPTKPDGTPRKLMASGKLFGMGWKPRVEFEEGLRAT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCHHHHHHHH
YRDFCARNGAGAAC
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]