Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 220903569

GI number: 220903569

Start: 343244

End: 344230

Strand: Reverse

Name: hemB [H]

Synonym: Ddes_0288

Alternate gene names: 220903569

Gene position: 344230-343244 (Counterclockwise)

Preceding gene: 220903570

Following gene: 220903568

Centisome position: 11.98

GC content: 61.7

Gene sequence:

>987_bases
ATGACTCCGTTCCATCGCGGCCGCCGTCTGCGTGCCACCCCCGAACTTCGCACCCTGGTGCGCGAAACTGCGCCCCTTCT
CGTGGAAGACCTTGTTCAGCCCTATTTTGTGGTTGAAACCGAAGACTCCGGTTTTCGCAAAGAAATCGGCTCCATGCCCG
GCCAGTACCAGCTGAGCCTCAGCCAGATGGAAAAACAGGTGGAGCAGGCCGTGGATACGGGCCTGCATTCGGTCATTCTT
TTCGGCATTCCCGCCACCAAGGACGAAAAAGCCTGCGGCGCCTACGCCGATGATGGCATCGTGCAGGAGGCCGTACGGCT
GCTCAAGCGCCGCTGGCCCAAACTTTTTGTCATGACAGACGTATGCCTGTGCGAATACATGAGCCACGGTCACTGTGGCA
TCCTTACGCCCGAGGGCGTGGTGCGCAACGACGCCACCCTGCCCCTGCTGGCGCAGACAGCCGTGAGCCACGCCAGGGCA
GGAGCCGACATGGTGGCCCCCTCGGACATGATGGACGGGCGCGTGGCCGCCATCCGCGAAGCGCTGGATCAGGCCGGTTT
TTCCCGGCTGCCGCTCATGTCCTATGCGGTCAAATACGCCTCGGCCTATTACGGACCTTTTCGCGAAGCCGCCGAAAGTG
CGCCTTCCAGCGGCGACCGAAAATCCTACCAGATGGACCCGGCCAACCTGCGCGAAGCCCTGCGTGAAGCCTATGCCGAC
CTTGAAGAAGGCGCGGACGCCCTTATCGTCAAGCCTGCCGGACCGTATGCCGACATCATCCGCCTTGTGCGCGATCATGT
GGACGTGCCGCTCTGCGCCTACCAGGTCAGCGGCGAATACTCGATGATACGCGCTGCCGGGCTTAACGGCTGGATAGACG
AACGGGCCGTCATGCTTGAATCCCTCATGGGGCTGAAGCGTGCCGGGGCGGACATGATCATCACCTACTTTACAGAAACC
CTGCTGCGCGAAAAGCTGGCAAGGTAG

Upstream 100 bases:

>100_bases
CCCTTGTTCAAAAAGGGGCCTCTTTCACATTTTCTGCCAGATTTTACAAGCGTTATTGCTATGATAGAGGCAGCCTGGCC
TGAAAAACAAGGATAGAGCC

Downstream 100 bases:

>100_bases
GGCGGAAGCATAATGAGCAAGCACATGCACCACGCCGGAGGGCACCCGGGCGGTATGCCCGGCAGCGCGGGCAACCCCAT
AAGCGGTCATCCCGGCCAGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTPFHRGRRLRATPELRTLVRETAPLLVEDLVQPYFVVETEDSGFRKEIGSMPGQYQLSLSQMEKQVEQAVDTGLHSVIL
FGIPATKDEKACGAYADDGIVQEAVRLLKRRWPKLFVMTDVCLCEYMSHGHCGILTPEGVVRNDATLPLLAQTAVSHARA
GADMVAPSDMMDGRVAAIREALDQAGFSRLPLMSYAVKYASAYYGPFREAAESAPSSGDRKSYQMDPANLREALREAYAD
LEEGADALIVKPAGPYADIIRLVRDHVDVPLCAYQVSGEYSMIRAAGLNGWIDERAVMLESLMGLKRAGADMIITYFTET
LLREKLAR

Sequences:

>Translated_328_residues
MTPFHRGRRLRATPELRTLVRETAPLLVEDLVQPYFVVETEDSGFRKEIGSMPGQYQLSLSQMEKQVEQAVDTGLHSVIL
FGIPATKDEKACGAYADDGIVQEAVRLLKRRWPKLFVMTDVCLCEYMSHGHCGILTPEGVVRNDATLPLLAQTAVSHARA
GADMVAPSDMMDGRVAAIREALDQAGFSRLPLMSYAVKYASAYYGPFREAAESAPSSGDRKSYQMDPANLREALREAYAD
LEEGADALIVKPAGPYADIIRLVRDHVDVPLCAYQVSGEYSMIRAAGLNGWIDERAVMLESLMGLKRAGADMIITYFTET
LLREKLAR
>Mature_327_residues
TPFHRGRRLRATPELRTLVRETAPLLVEDLVQPYFVVETEDSGFRKEIGSMPGQYQLSLSQMEKQVEQAVDTGLHSVILF
GIPATKDEKACGAYADDGIVQEAVRLLKRRWPKLFVMTDVCLCEYMSHGHCGILTPEGVVRNDATLPLLAQTAVSHARAG
ADMVAPSDMMDGRVAAIREALDQAGFSRLPLMSYAVKYASAYYGPFREAAESAPSSGDRKSYQMDPANLREALREAYADL
EEGADALIVKPAGPYADIIRLVRDHVDVPLCAYQVSGEYSMIRAAGLNGWIDERAVMLESLMGLKRAGADMIITYFTETL
LREKLAR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=314, Percent_Identity=44.2675159235669, Blast_Score=266, Evalue=2e-71,
Organism=Escherichia coli, GI87081728, Length=325, Percent_Identity=50.4615384615385, Blast_Score=302, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=299, Percent_Identity=43.4782608695652, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21358291, Length=310, Percent_Identity=42.9032258064516, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36253; Mature: 36122

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFHRGRRLRATPELRTLVRETAPLLVEDLVQPYFVVETEDSGFRKEIGSMPGQYQLSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCEEEEH
SQMEKQVEQAVDTGLHSVILFGIPATKDEKACGAYADDGIVQEAVRLLKRRWPKLFVMTD
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEHHHHH
VCLCEYMSHGHCGILTPEGVVRNDATLPLLAQTAVSHARAGADMVAPSDMMDGRVAAIRE
HHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHH
ALDQAGFSRLPLMSYAVKYASAYYGPFREAAESAPSSGDRKSYQMDPANLREALREAYAD
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LEEGADALIVKPAGPYADIIRLVRDHVDVPLCAYQVSGEYSMIRAAGLNGWIDERAVMLE
HHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHH
SLMGLKRAGADMIITYFTETLLREKLAR
HHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPFHRGRRLRATPELRTLVRETAPLLVEDLVQPYFVVETEDSGFRKEIGSMPGQYQLSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCEEEEH
SQMEKQVEQAVDTGLHSVILFGIPATKDEKACGAYADDGIVQEAVRLLKRRWPKLFVMTD
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEHHHHH
VCLCEYMSHGHCGILTPEGVVRNDATLPLLAQTAVSHARAGADMVAPSDMMDGRVAAIRE
HHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHH
ALDQAGFSRLPLMSYAVKYASAYYGPFREAAESAPSSGDRKSYQMDPANLREALREAYAD
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LEEGADALIVKPAGPYADIIRLVRDHVDVPLCAYQVSGEYSMIRAAGLNGWIDERAVMLE
HHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHH
SLMGLKRAGADMIITYFTETLLREKLAR
HHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]