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The map label for this gene is gap [H]

Identifier: 220903442

GI number: 220903442

Start: 196044

End: 197060

Strand: Reverse

Name: gap [H]

Synonym: Ddes_0156

Alternate gene names: 220903442

Gene position: 197060-196044 (Counterclockwise)

Preceding gene: 220903444

Following gene: 220903441

Centisome position: 6.86

GC content: 58.01

Gene sequence:

>1017_bases
ATGAGCAAGATCAGAATAGGCATCAACGGCTTCGGCCGCATCGGCAGGCAGGTTTTCCGCGCCCTGCACCAGCACTACCG
CGACCGTGTGGAAGTGGTAGCCATCAACGACCTTTTTGACGCCGAAACCAACTTTCACCTGCTGGAATACGACTCGGTTT
ACGGGCGCGCGCACCTGGACGCCAAAATAGACGGCGCGGAAGTGACCGTGGGCGACTGGAAGATACACTGCTTTGCCGAA
CGTGACCCCAAACAGCTTGCCTGGGGCCAGTACGGCGTGGACATCGTTGTGGAAAGCACGGGTATTTTCCGCAAGGCTTC
GCAGTGCTCGGTGCATATTGAAAACGGCGCGAAAAAAGTCATCATCACCGCCCCCGCCAAGGAAGAAGACATCACCATCG
TCATGGGCGTGAACCATCAGGAATACGATCCGGCCAAACACCATATCGTGTCCAACGCCTCCTGCACCACCAACTGCCTG
GCCCCCGTGGCCCTGGTGCTGCAAAAGGAATTCGGCATCCAGATCGGCAACATGACCACCATCCACTCCTACACCAACGA
CCAGCGCATCCTGGACATGGCCCACAAGGACCCGCGCCGCGCCCGCGCCGCCGCCTGCAATATCATTCCCACGTCCACGG
GAGCCGCCCAGGCCGTTGCCAAGGTCATTCCCGAACTGAAGGGCAAGTTCAGCGGTTATTCGCTGCGTGTGCCCACGCCC
ACGGTATCGGTGGTGGACTTTACCGGCATTCTGGAAAAGGAAACCGACACGGAAACCCTGCTGGGCAAACTCAAGGAAGC
CTCTGAAACCTATCTCAAGGGCATTCTTGAATATAATGACAAAGCACTGGTTTCAATGGACTTCAAGGGCAATCCGGCCT
CGTCCATTCTTGAGGCTTCCTATACCACGGTGCAGGACGGCAAGATGGTCAAGCTGGTGGCCTGGTACGACAACGAGTGG
GGCTACTCCTGCCGCGTGTGCGACCTCGCCTGCCTCATGGCCGAAAAAGGCCTGTAG

Upstream 100 bases:

>100_bases
GCCTCAAGAACCGCCCCATTGGCCGTCAGCAGACCTGCTCCGGCTGGAAAGGCGCACGGCAGCATACGACATTTCCCTCA
ACAGCATGAGGATTGCGCAT

Downstream 100 bases:

>100_bases
AGCCGGTTCAGCTTTTCTGCTCCGCCCGGCCGTTGCCATGCAGCGCCGCGCAACAAAAAAGCTCTGCCGCGCAGTGTCGC
GTTTCCTGTCGGCCGCGTCT

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSKIRIGINGFGRIGRQVFRALHQHYRDRVEVVAINDLFDAETNFHLLEYDSVYGRAHLDAKIDGAEVTVGDWKIHCFAE
RDPKQLAWGQYGVDIVVESTGIFRKASQCSVHIENGAKKVIITAPAKEEDITIVMGVNHQEYDPAKHHIVSNASCTTNCL
APVALVLQKEFGIQIGNMTTIHSYTNDQRILDMAHKDPRRARAAACNIIPTSTGAAQAVAKVIPELKGKFSGYSLRVPTP
TVSVVDFTGILEKETDTETLLGKLKEASETYLKGILEYNDKALVSMDFKGNPASSILEASYTTVQDGKMVKLVAWYDNEW
GYSCRVCDLACLMAEKGL

Sequences:

>Translated_338_residues
MSKIRIGINGFGRIGRQVFRALHQHYRDRVEVVAINDLFDAETNFHLLEYDSVYGRAHLDAKIDGAEVTVGDWKIHCFAE
RDPKQLAWGQYGVDIVVESTGIFRKASQCSVHIENGAKKVIITAPAKEEDITIVMGVNHQEYDPAKHHIVSNASCTTNCL
APVALVLQKEFGIQIGNMTTIHSYTNDQRILDMAHKDPRRARAAACNIIPTSTGAAQAVAKVIPELKGKFSGYSLRVPTP
TVSVVDFTGILEKETDTETLLGKLKEASETYLKGILEYNDKALVSMDFKGNPASSILEASYTTVQDGKMVKLVAWYDNEW
GYSCRVCDLACLMAEKGL
>Mature_337_residues
SKIRIGINGFGRIGRQVFRALHQHYRDRVEVVAINDLFDAETNFHLLEYDSVYGRAHLDAKIDGAEVTVGDWKIHCFAER
DPKQLAWGQYGVDIVVESTGIFRKASQCSVHIENGAKKVIITAPAKEEDITIVMGVNHQEYDPAKHHIVSNASCTTNCLA
PVALVLQKEFGIQIGNMTTIHSYTNDQRILDMAHKDPRRARAAACNIIPTSTGAAQAVAKVIPELKGKFSGYSLRVPTPT
VSVVDFTGILEKETDTETLLGKLKEASETYLKGILEYNDKALVSMDFKGNPASSILEASYTTVQDGKMVKLVAWYDNEWG
YSCRVCDLACLMAEKGL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=338, Percent_Identity=47.0414201183432, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI7657116, Length=336, Percent_Identity=45.5357142857143, Blast_Score=282, Evalue=3e-76,
Organism=Escherichia coli, GI1788079, Length=327, Percent_Identity=48.9296636085627, Blast_Score=303, Evalue=7e-84,
Organism=Escherichia coli, GI1789295, Length=333, Percent_Identity=39.9399399399399, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17534677, Length=344, Percent_Identity=47.9651162790698, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17534679, Length=344, Percent_Identity=47.3837209302326, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32566163, Length=343, Percent_Identity=47.8134110787172, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17568413, Length=343, Percent_Identity=47.8134110787172, Blast_Score=296, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=49.1017964071856, Blast_Score=303, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6322468, Length=328, Percent_Identity=49.6951219512195, Blast_Score=300, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321631, Length=329, Percent_Identity=50.4559270516717, Blast_Score=298, Evalue=7e-82,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=47.3053892215569, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=47.3053892215569, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=47.0059880239521, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=47.0059880239521, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=45.0920245398773, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 37383; Mature: 37252

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRIGINGFGRIGRQVFRALHQHYRDRVEVVAINDLFDAETNFHLLEYDSVYGRAHLD
CCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCEEEEC
AKIDGAEVTVGDWKIHCFAERDPKQLAWGQYGVDIVVESTGIFRKASQCSVHIENGAKKV
EEECCCEEEEECEEEEEEECCCCHHHCCCCCCEEEEEECCCCEEECCCCEEEEECCCEEE
IITAPAKEEDITIVMGVNHQEYDPAKHHIVSNASCTTNCLAPVALVLQKEFGIQIGNMTT
EEECCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHCEEECCEEE
IHSYTNDQRILDMAHKDPRRARAAACNIIPTSTGAAQAVAKVIPELKGKFSGYSLRVPTP
EECCCCCHHHHHHHCCCHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCC
TVSVVDFTGILEKETDTETLLGKLKEASETYLKGILEYNDKALVSMDFKGNPASSILEAS
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCC
YTTVQDGKMVKLVAWYDNEWGYSCRVCDLACLMAEKGL
CEEECCCCEEEEEEEECCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIRIGINGFGRIGRQVFRALHQHYRDRVEVVAINDLFDAETNFHLLEYDSVYGRAHLD
CEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCEEEEC
AKIDGAEVTVGDWKIHCFAERDPKQLAWGQYGVDIVVESTGIFRKASQCSVHIENGAKKV
EEECCCEEEEECEEEEEEECCCCHHHCCCCCCEEEEEECCCCEEECCCCEEEEECCCEEE
IITAPAKEEDITIVMGVNHQEYDPAKHHIVSNASCTTNCLAPVALVLQKEFGIQIGNMTT
EEECCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHCEEECCEEE
IHSYTNDQRILDMAHKDPRRARAAACNIIPTSTGAAQAVAKVIPELKGKFSGYSLRVPTP
EECCCCCHHHHHHHCCCHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCC
TVSVVDFTGILEKETDTETLLGKLKEASETYLKGILEYNDKALVSMDFKGNPASSILEAS
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCC
YTTVQDGKMVKLVAWYDNEWGYSCRVCDLACLMAEKGL
CEEECCCCEEEEEEEECCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]