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The map label for this gene is glsA

Identifier: 220903334

GI number: 220903334

Start: 64339

End: 65271

Strand: Direct

Name: glsA

Synonym: Ddes_0048

Alternate gene names: 220903334

Gene position: 64339-65271 (Clockwise)

Preceding gene: 220903333

Following gene: 220903336

Centisome position: 2.24

GC content: 59.16

Gene sequence:

>933_bases
ATGCCTGCTATTGAAAAAATCCAGTCTGTGGTGAACAGCGCGTACGCGCAATTCAGCAAGGCCTCCGGCGGGGCCAATGC
CGATTACATACCTTTTCTGGCAAATATACCGAGCGACCTTGCAGCCGTGGCTGTGGTGACGGCCCAGGGGCAGGTGGCGG
CGGCTGGCGATGCGCAATACCGCTTTGCCATCGAATCCATTTCAAAGGTCTGCACGCTGGCGCTGGCGCTGGAAGACTTC
GGCCCGCAGGCCGTACAGGAAAAGATAGGCACCAGCCCTACGGGGCTGCCCTTTAATTCGGTTATGGCGCTGGAACTGCA
CGGCGACAAACCCCTTTCTCCGCTGGTGAACGCCGGGGCCATGGCCTCGGCCAGCCTTGTGAAGGCAGGCTCCACAGAAG
AGCGCTGGCAGCGGATATTGGACATGCAGCGCAGGCTAGGCTCAAAGGAAATAGCCATTTCTGACGAACTGAACCAGTCG
GAGCAGACCACAAACTTTCACAACCGGGGCATTGCCTGGCTGCTGTATTCCGCAGGCTATATGTACTGCGACCCCATGGA
AGCCTGCGATGTATACACACGCCAGTGCTCGACCCTGCTCAATACCGTGGAGCTTGCTACTGTCGGCGCTACCATTGCCG
CACGCGGGCGCAACCCCGTTACCGGCGAACAGGTGCTCAAGCCCGAGCACTGCCCCTGCATCATGGCTGAAATGACCATG
GAGGGCATGTATGACAGTTCTGGCGACTGGGCGTACAAGGTGGGCCTGCCGGGCAAGAGCGGCGTTGGAGGCGGCATATT
GACGATTGTGCCGGGCATTATGGCCATCGCGGCCTTTTCGCCGCCTCTGGACAGCGTGGGCAACAGTGTGCGCGGGCAGA
AGATGGCGGCCTTTGTGGCCAATGAACTGGGATATAATCTGTATAAAGCCTGA

Upstream 100 bases:

>100_bases
CAGGCCGGAAGCTAGCGCCCGGTATTTCATGACAAGATAACAGTTTGTAATAAAGCCGTAATAGCTTTGCTATTGCGGCT
TTATTTAAAAGGAAAACACC

Downstream 100 bases:

>100_bases
TAGGCTGGCTCACCGCGCGTCGGGCACAGCTTGTGCCTCCGGCGCTGGGTGTTTGCCCTTTTCGGCCCCCCGGCAGGTAT
GCAGGGGGGCCGTTTTTTGC

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPAIEKIQSVVNSAYAQFSKASGGANADYIPFLANIPSDLAAVAVVTAQGQVAAAGDAQYRFAIESISKVCTLALALEDF
GPQAVQEKIGTSPTGLPFNSVMALELHGDKPLSPLVNAGAMASASLVKAGSTEERWQRILDMQRRLGSKEIAISDELNQS
EQTTNFHNRGIAWLLYSAGYMYCDPMEACDVYTRQCSTLLNTVELATVGATIAARGRNPVTGEQVLKPEHCPCIMAEMTM
EGMYDSSGDWAYKVGLPGKSGVGGGILTIVPGIMAIAAFSPPLDSVGNSVRGQKMAAFVANELGYNLYKA

Sequences:

>Translated_310_residues
MPAIEKIQSVVNSAYAQFSKASGGANADYIPFLANIPSDLAAVAVVTAQGQVAAAGDAQYRFAIESISKVCTLALALEDF
GPQAVQEKIGTSPTGLPFNSVMALELHGDKPLSPLVNAGAMASASLVKAGSTEERWQRILDMQRRLGSKEIAISDELNQS
EQTTNFHNRGIAWLLYSAGYMYCDPMEACDVYTRQCSTLLNTVELATVGATIAARGRNPVTGEQVLKPEHCPCIMAEMTM
EGMYDSSGDWAYKVGLPGKSGVGGGILTIVPGIMAIAAFSPPLDSVGNSVRGQKMAAFVANELGYNLYKA
>Mature_309_residues
PAIEKIQSVVNSAYAQFSKASGGANADYIPFLANIPSDLAAVAVVTAQGQVAAAGDAQYRFAIESISKVCTLALALEDFG
PQAVQEKIGTSPTGLPFNSVMALELHGDKPLSPLVNAGAMASASLVKAGSTEERWQRILDMQRRLGSKEIAISDELNQSE
QTTNFHNRGIAWLLYSAGYMYCDPMEACDVYTRQCSTLLNTVELATVGATIAARGRNPVTGEQVLKPEHCPCIMAEMTME
GMYDSSGDWAYKVGLPGKSGVGGGILTIVPGIMAIAAFSPPLDSVGNSVRGQKMAAFVANELGYNLYKA

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=296, Percent_Identity=37.1621621621622, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI20336214, Length=312, Percent_Identity=33.0128205128205, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI1786693, Length=304, Percent_Identity=72.0394736842105, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1787804, Length=286, Percent_Identity=38.1118881118881, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17507019, Length=314, Percent_Identity=33.7579617834395, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17532727, Length=313, Percent_Identity=32.5878594249201, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193204073, Length=322, Percent_Identity=30.1242236024845, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI193204075, Length=324, Percent_Identity=29.9382716049383, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI281363241, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24653164, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24653162, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24653156, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI281363239, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24653158, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24653166, Length=319, Percent_Identity=31.6614420062696, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI116008307, Length=319, Percent_Identity=31.6614420062696, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA_DESDA (B8J1X3)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002478646.1
- ProteinModelPortal:   B8J1X3
- GeneID:   7283699
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0048
- HOGENOM:   HBG512335
- ProtClustDB:   PRK12356
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 32784; Mature: 32653

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIEKIQSVVNSAYAQFSKASGGANADYIPFLANIPSDLAAVAVVTAQGQVAAAGDAQY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHEEEEECCCCEEECCCCHH
RFAIESISKVCTLALALEDFGPQAVQEKIGTSPTGLPFNSVMALELHGDKPLSPLVNAGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHCCCC
MASASLVKAGSTEERWQRILDMQRRLGSKEIAISDELNQSEQTTNFHNRGIAWLLYSAGY
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHCCHHHCCEEEEEECCCC
MYCDPMEACDVYTRQCSTLLNTVELATVGATIAARGRNPVTGEQVLKPEHCPCIMAEMTM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCHHHHHHH
EGMYDSSGDWAYKVGLPGKSGVGGGILTIVPGIMAIAAFSPPLDSVGNSVRGQKMAAFVA
HHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHH
NELGYNLYKA
HHHCCHHHCC
>Mature Secondary Structure 
PAIEKIQSVVNSAYAQFSKASGGANADYIPFLANIPSDLAAVAVVTAQGQVAAAGDAQY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHEEEEECCCCEEECCCCHH
RFAIESISKVCTLALALEDFGPQAVQEKIGTSPTGLPFNSVMALELHGDKPLSPLVNAGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHCCCC
MASASLVKAGSTEERWQRILDMQRRLGSKEIAISDELNQSEQTTNFHNRGIAWLLYSAGY
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHCCHHHCCEEEEEECCCC
MYCDPMEACDVYTRQCSTLLNTVELATVGATIAARGRNPVTGEQVLKPEHCPCIMAEMTM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCHHHHHHH
EGMYDSSGDWAYKVGLPGKSGVGGGILTIVPGIMAIAAFSPPLDSVGNSVRGQKMAAFVA
HHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHH
NELGYNLYKA
HHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA