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The map label for this gene is xasA [C]

Identifier: 220903333

GI number: 220903333

Start: 62754

End: 64253

Strand: Direct

Name: xasA [C]

Synonym: Ddes_0047

Alternate gene names: 220903333

Gene position: 62754-64253 (Clockwise)

Preceding gene: 220903330

Following gene: 220903334

Centisome position: 2.18

GC content: 56.87

Gene sequence:

>1500_bases
ATGGGAGAAGTGAAAGAACAAACTTCCGGTTCAAAGATGACTATTACGACCATAGTTATTATGAATATTACTGCGGTCGT
CAGCCTGCGTTTTCTTCCTTCAGAGGCTGAATACGGCTTGGGCGCCATTTTTTACTATGCCTTTGCGGCCCTGGTCTTTC
TGGTTCCCATGGCGCTGGTAGCGGCGGAACTGGCTACCACCTATCCTGAAAAAGGCGGTGTTTTCCGCTGGGTCAGCGAG
GCGTTCGGTCCGCGCTGGGGCTTTTTGGCCATGGCCATGCTGTGGATTGAAGTCATTCCCTACTTTCCCACAGTGCTCAC
CTTCGGGGCCGTATCTATCGCCTTTATGGACCCGAACGTAAGTATGGCAGAAACGATGGCGGCCAATAAATGGTATATTA
CGGGCTTCGTTCTGCTTGTGTACTGGATATCGGTATTCATTGCCCTGCGCGGCGTCGGCATATTTGCGCGGGTGTCAAAA
TGGTGCGGTATCGTGGGTACGATTATTCCCGCCGCAGTGGTGGTGGTGCTGGGTTTCGCCTATCTGTTCTGCAGCGGAAA
GCCGCCGTTGATTGAGCTGAGCTGGGGAGCGCTGATCCCCGACTTTACCAATTTCAGCAACGTGGTTCTTGCCGCCAGCA
TATTCCTGGCTTACGCGGGTATGGAAATGAACGCCGTGCACGTTAAGGATATGGACAATCCCACGAAAAAATATCCCATA
GCCATTACCATCGCCTCGGTGGGCACGGTCGCCATTTTCCTGCTGAGCACCCTGGGTATCGCCTTTGTTGTGCCCAAGCA
GGACATCAACCTTACCCAGAGCCTGCTGCTGGCCTATGACCTGCTGTTCAAGTGGATCAACGCCGAGTGGCTGGGGTCCG
TGCTGGCCGTCATGCTGGCTTTCGGCGTGCTTGGCGGCGTGGTCACCTGGATCGCCGGTCCCAATACCGGTGTGCTTGCC
GTGGCCAAGGCCGGGTATCTGCCCCGCTGGTTCCAGAAGACCAACCGCTTCGGCATGGGCAGCCATCTCATGATCGTGCA
GGCGGTGGTTGTTACCATCCTGTCCATCACCTTTGTGGTCATGCCTTCAGTGCAGGCGGCCTTTCAGATTCTGTCGCAGC
TTACGGTGATCCTGTACCTGGTCATGTACATGCTCATGTTCGCCAGCGGCATCCGCCTGCGGCTGAGCCAGCCCAACCGG
CCGCGGCCCTATCGGGTTCCCGGCATGTACCTGTGGGCTACGCTGGGCTTTCTCGGCTCGCTGCTGGCCTTTACGCTCAG
CTTTGTGCCTCCGAGCCAGATTTCCATCGGCAGCCCTGAAACCTACATGATGTACCTGATCGTGCTGGTGGTGATCTTTG
TGGCTATCCCGCTGACGATCTTCGCCCTGCGCAAGCCTGAATGGCGCGACCCCAATTCAGACTTTGAGCCGTTCACCTGG
GAAAAGGAAAAGGCCCAGGCCGCTGCGCAGCCGCAGACCGGAACGCAGGCCGGAAGCTAG

Upstream 100 bases:

>100_bases
TAAAAGTGATTATTGATCCCCGCTGCGTTTTCTGCTTCGCGGCAGCCCGTGCATCAAACACAGTGGCGTTAATCGACTCT
GTTAACATAGGAGCGTCATT

Downstream 100 bases:

>100_bases
CGCCCGGTATTTCATGACAAGATAACAGTTTGTAATAAAGCCGTAATAGCTTTGCTATTGCGGCTTTATTTAAAAGGAAA
ACACCATGCCTGCTATTGAA

Product: amino acid permease-associated protein

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MGEVKEQTSGSKMTITTIVIMNITAVVSLRFLPSEAEYGLGAIFYYAFAALVFLVPMALVAAELATTYPEKGGVFRWVSE
AFGPRWGFLAMAMLWIEVIPYFPTVLTFGAVSIAFMDPNVSMAETMAANKWYITGFVLLVYWISVFIALRGVGIFARVSK
WCGIVGTIIPAAVVVVLGFAYLFCSGKPPLIELSWGALIPDFTNFSNVVLAASIFLAYAGMEMNAVHVKDMDNPTKKYPI
AITIASVGTVAIFLLSTLGIAFVVPKQDINLTQSLLLAYDLLFKWINAEWLGSVLAVMLAFGVLGGVVTWIAGPNTGVLA
VAKAGYLPRWFQKTNRFGMGSHLMIVQAVVVTILSITFVVMPSVQAAFQILSQLTVILYLVMYMLMFASGIRLRLSQPNR
PRPYRVPGMYLWATLGFLGSLLAFTLSFVPPSQISIGSPETYMMYLIVLVVIFVAIPLTIFALRKPEWRDPNSDFEPFTW
EKEKAQAAAQPQTGTQAGS

Sequences:

>Translated_499_residues
MGEVKEQTSGSKMTITTIVIMNITAVVSLRFLPSEAEYGLGAIFYYAFAALVFLVPMALVAAELATTYPEKGGVFRWVSE
AFGPRWGFLAMAMLWIEVIPYFPTVLTFGAVSIAFMDPNVSMAETMAANKWYITGFVLLVYWISVFIALRGVGIFARVSK
WCGIVGTIIPAAVVVVLGFAYLFCSGKPPLIELSWGALIPDFTNFSNVVLAASIFLAYAGMEMNAVHVKDMDNPTKKYPI
AITIASVGTVAIFLLSTLGIAFVVPKQDINLTQSLLLAYDLLFKWINAEWLGSVLAVMLAFGVLGGVVTWIAGPNTGVLA
VAKAGYLPRWFQKTNRFGMGSHLMIVQAVVVTILSITFVVMPSVQAAFQILSQLTVILYLVMYMLMFASGIRLRLSQPNR
PRPYRVPGMYLWATLGFLGSLLAFTLSFVPPSQISIGSPETYMMYLIVLVVIFVAIPLTIFALRKPEWRDPNSDFEPFTW
EKEKAQAAAQPQTGTQAGS
>Mature_498_residues
GEVKEQTSGSKMTITTIVIMNITAVVSLRFLPSEAEYGLGAIFYYAFAALVFLVPMALVAAELATTYPEKGGVFRWVSEA
FGPRWGFLAMAMLWIEVIPYFPTVLTFGAVSIAFMDPNVSMAETMAANKWYITGFVLLVYWISVFIALRGVGIFARVSKW
CGIVGTIIPAAVVVVLGFAYLFCSGKPPLIELSWGALIPDFTNFSNVVLAASIFLAYAGMEMNAVHVKDMDNPTKKYPIA
ITIASVGTVAIFLLSTLGIAFVVPKQDINLTQSLLLAYDLLFKWINAEWLGSVLAVMLAFGVLGGVVTWIAGPNTGVLAV
AKAGYLPRWFQKTNRFGMGSHLMIVQAVVVTILSITFVVMPSVQAAFQILSQLTVILYLVMYMLMFASGIRLRLSQPNRP
RPYRVPGMYLWATLGFLGSLLAFTLSFVPPSQISIGSPETYMMYLIVLVVIFVAIPLTIFALRKPEWRDPNSDFEPFTWE
KEKAQAAAQPQTGTQAGS

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=493, Percent_Identity=30.0202839756592, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87081798, Length=392, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 54684; Mature: 54552

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVKEQTSGSKMTITTIVIMNITAVVSLRFLPSEAEYGLGAIFYYAFAALVFLVPMALV
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELATTYPEKGGVFRWVSEAFGPRWGFLAMAMLWIEVIPYFPTVLTFGAVSIAFMDPNV
HHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
SMAETMAANKWYITGFVLLVYWISVFIALRGVGIFARVSKWCGIVGTIIPAAVVVVLGFA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLFCSGKPPLIELSWGALIPDFTNFSNVVLAASIFLAYAGMEMNAVHVKDMDNPTKKYPI
HHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
AITIASVGTVAIFLLSTLGIAFVVPKQDINLTQSLLLAYDLLFKWINAEWLGSVLAVMLA
EEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FGVLGGVVTWIAGPNTGVLAVAKAGYLPRWFQKTNRFGMGSHLMIVQAVVVTILSITFVV
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MPSVQAAFQILSQLTVILYLVMYMLMFASGIRLRLSQPNRPRPYRVPGMYLWATLGFLGS
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
LLAFTLSFVPPSQISIGSPETYMMYLIVLVVIFVAIPLTIFALRKPEWRDPNSDFEPFTW
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EKEKAQAAAQPQTGTQAGS
CHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GEVKEQTSGSKMTITTIVIMNITAVVSLRFLPSEAEYGLGAIFYYAFAALVFLVPMALV
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELATTYPEKGGVFRWVSEAFGPRWGFLAMAMLWIEVIPYFPTVLTFGAVSIAFMDPNV
HHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
SMAETMAANKWYITGFVLLVYWISVFIALRGVGIFARVSKWCGIVGTIIPAAVVVVLGFA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLFCSGKPPLIELSWGALIPDFTNFSNVVLAASIFLAYAGMEMNAVHVKDMDNPTKKYPI
HHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
AITIASVGTVAIFLLSTLGIAFVVPKQDINLTQSLLLAYDLLFKWINAEWLGSVLAVMLA
EEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FGVLGGVVTWIAGPNTGVLAVAKAGYLPRWFQKTNRFGMGSHLMIVQAVVVTILSITFVV
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MPSVQAAFQILSQLTVILYLVMYMLMFASGIRLRLSQPNRPRPYRVPGMYLWATLGFLGS
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
LLAFTLSFVPPSQISIGSPETYMMYLIVLVVIFVAIPLTIFALRKPEWRDPNSDFEPFTW
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EKEKAQAAAQPQTGTQAGS
CHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]