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The map label for this gene is rfbB [C]

Identifier: 220903311

GI number: 220903311

Start: 36086

End: 37102

Strand: Reverse

Name: rfbB [C]

Synonym: Ddes_0025

Alternate gene names: 220903311

Gene position: 37102-36086 (Counterclockwise)

Preceding gene: 220903313

Following gene: 220903310

Centisome position: 1.29

GC content: 59.49

Gene sequence:

>1017_bases
ATGCACGTTCTGGTAACCGGCGCGGCAGGATTCATCGGCTATCATCTGGCTGCACGGCTGCTGGCCGACGGGCACAGCGT
GGTGGGCATTGACAATTGCAACGATTACTACGACGTGCAGTTGAAAAAAGACCGCCTGGCGCAACTGGCCGCCTTGCCCC
AGGCCTCGTGCTTTCGTTTCGAACCGCTGGACCTGGCCGACGGGCCGGGCATGTCCGCCCTTTTCGCGCGCGAAGGTTTC
AGCCATGTTGTGAATCTGGCCGCGCAGGCCGGGGTGCGCTACAGCCTCGTCAATCCCGAATCCTATCTCAGCTCCAACCT
GACAGGCTTTGGTCATGTGCTTGAAGGCTGCCGCCACAACAAGGTCGGCCACCTGCTTTTTGCCTCATCTTCTTCGGTCT
ACGGGCTCAACGCGGCCCAACCCTACTCTGTACGGCACAATGTGGACCATCCGGTTAGCCTGTATGCGGCCACCAAAAAA
AGCAACGAGCTTATGGCCCATTCCTACAGCCATCTCTACGGCATCCCCTGCACCGGGCTGCGTTTCTTCACCGTATACGG
CCCGTGGGGCAGGCCGGACATGGCCCTGCACCTTTTTACCACCGCCATTGTGCGCGGCGAACCCATCAAGGTCTTTAACG
AAGGCCGCATGCGCCGCGACTTCACCTATATTGACGACATAGTGGAAGGCATTGTACGCCTGCTGCCCCTGGCCCCCGGC
CCAGACCCGGCCTTTGACCCGGCAGCGCCCGACCCGGCTTCCAGTTCCGCGCCCTGGCGCATTTATAATATCGGCAATAA
CAATACGGTGGAACTCAACGATTTTATCGCCACCCTTGAAGACGCGCTGGGCATGAAAGCCCGCAAGGACATGCTGCCCA
TGCAGCCCGGCGACGTAAAGGCCACCTGGGCCGACATCAACGACCTCACCGCCCTTACGGGTTTTGCCCCGTCGACGCCC
TTGCGCGAGGGCATCGCCCGCTTTGTGGAATGGTATAAAGAGTATTATAAAATATGA

Upstream 100 bases:

>100_bases
GGCAGATTTTCATCAAAGCCACCCCGAGCCTGCGGCCTTTGCTTGCTCACGAACGGCATCTGGCTTACCTTGCCTGCATG
CACAACTGGAGGAAAGTCAT

Downstream 100 bases:

>100_bases
GTGATTCTTCAATTATTCCGGCGTCTGGCGCTGTTACTGTTCCCACTCCGGCCCGGCCGGAAATTCTGGCCCCTGCAGGA
GACACTCCGTCTTTTCTGGC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MHVLVTGAAGFIGYHLAARLLADGHSVVGIDNCNDYYDVQLKKDRLAQLAALPQASCFRFEPLDLADGPGMSALFAREGF
SHVVNLAAQAGVRYSLVNPESYLSSNLTGFGHVLEGCRHNKVGHLLFASSSSVYGLNAAQPYSVRHNVDHPVSLYAATKK
SNELMAHSYSHLYGIPCTGLRFFTVYGPWGRPDMALHLFTTAIVRGEPIKVFNEGRMRRDFTYIDDIVEGIVRLLPLAPG
PDPAFDPAAPDPASSSAPWRIYNIGNNNTVELNDFIATLEDALGMKARKDMLPMQPGDVKATWADINDLTALTGFAPSTP
LREGIARFVEWYKEYYKI

Sequences:

>Translated_338_residues
MHVLVTGAAGFIGYHLAARLLADGHSVVGIDNCNDYYDVQLKKDRLAQLAALPQASCFRFEPLDLADGPGMSALFAREGF
SHVVNLAAQAGVRYSLVNPESYLSSNLTGFGHVLEGCRHNKVGHLLFASSSSVYGLNAAQPYSVRHNVDHPVSLYAATKK
SNELMAHSYSHLYGIPCTGLRFFTVYGPWGRPDMALHLFTTAIVRGEPIKVFNEGRMRRDFTYIDDIVEGIVRLLPLAPG
PDPAFDPAAPDPASSSAPWRIYNIGNNNTVELNDFIATLEDALGMKARKDMLPMQPGDVKATWADINDLTALTGFAPSTP
LREGIARFVEWYKEYYKI
>Mature_338_residues
MHVLVTGAAGFIGYHLAARLLADGHSVVGIDNCNDYYDVQLKKDRLAQLAALPQASCFRFEPLDLADGPGMSALFAREGF
SHVVNLAAQAGVRYSLVNPESYLSSNLTGFGHVLEGCRHNKVGHLLFASSSSVYGLNAAQPYSVRHNVDHPVSLYAATKK
SNELMAHSYSHLYGIPCTGLRFFTVYGPWGRPDMALHLFTTAIVRGEPIKVFNEGRMRRDFTYIDDIVEGIVRLLPLAPG
PDPAFDPAAPDPASSSAPWRIYNIGNNNTVELNDFIATLEDALGMKARKDMLPMQPGDVKATWADINDLTALTGFAPSTP
LREGIARFVEWYKEYYKI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=26.0233918128655, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=24.9266862170088, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=26.2108262108262, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=26.2108262108262, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=26.2108262108262, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=23.8888888888889, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=23.0113636363636, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786974, Length=318, Percent_Identity=25.4716981132075, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=24.5762711864407, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=24.438202247191, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=342, Percent_Identity=22.8070175438596, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=246, Percent_Identity=28.0487804878049, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19923002, Length=322, Percent_Identity=25.776397515528, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356223, Length=340, Percent_Identity=24.1176470588235, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37124; Mature: 37124

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLVTGAAGFIGYHLAARLLADGHSVVGIDNCNDYYDVQLKKDRLAQLAALPQASCFRF
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECHHHHHHHHHCCCCCCEEE
EPLDLADGPGMSALFAREGFSHVVNLAAQAGVRYSLVNPESYLSSNLTGFGHVLEGCRHN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHCCC
KVGHLLFASSSSVYGLNAAQPYSVRHNVDHPVSLYAATKKSNELMAHSYSHLYGIPCTGL
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHEECCCCCCE
RFFTVYGPWGRPDMALHLFTTAIVRGEPIKVFNEGRMRRDFTYIDDIVEGIVRLLPLAPG
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
PDPAFDPAAPDPASSSAPWRIYNIGNNNTVELNDFIATLEDALGMKARKDMLPMQPGDVK
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCHHHHHCCCCCCCCCE
ATWADINDLTALTGFAPSTPLREGIARFVEWYKEYYKI
EEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVLVTGAAGFIGYHLAARLLADGHSVVGIDNCNDYYDVQLKKDRLAQLAALPQASCFRF
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECHHHHHHHHHCCCCCCEEE
EPLDLADGPGMSALFAREGFSHVVNLAAQAGVRYSLVNPESYLSSNLTGFGHVLEGCRHN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHCCC
KVGHLLFASSSSVYGLNAAQPYSVRHNVDHPVSLYAATKKSNELMAHSYSHLYGIPCTGL
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHEECCCCCCE
RFFTVYGPWGRPDMALHLFTTAIVRGEPIKVFNEGRMRRDFTYIDDIVEGIVRLLPLAPG
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
PDPAFDPAAPDPASSSAPWRIYNIGNNNTVELNDFIATLEDALGMKARKDMLPMQPGDVK
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCHHHHHCCCCCCCCCE
ATWADINDLTALTGFAPSTPLREGIARFVEWYKEYYKI
EEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]