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The map label for this gene is def [H]

Identifier: 220903297

GI number: 220903297

Start: 16332

End: 16850

Strand: Direct

Name: def [H]

Synonym: Ddes_0011

Alternate gene names: 220903297

Gene position: 16332-16850 (Clockwise)

Preceding gene: 220903296

Following gene: 220903298

Centisome position: 0.57

GC content: 54.72

Gene sequence:

>519_bases
ATGATTCTTGATATTGTTACCTATCCTGATCCGCGCCTCAAAGAGGTGTGCGAGCCTGTTGCGGAAGTGACGGACGAAAT
CCGCCAGCTTGCCGCTGACATGCTCGAAACCATGTATGAGGCCCCCGGCGTGGGACTTGCCGCGCCTCAGGTGGGGCGCA
ATATACGCATGCTGGTTATGGACCCTTCTGCCCAGAAAGATGAACGCAACCCCCGTGTGCTTATCAACCCTGTGCTGACG
CTCTCTGGCGAAGAGATCATCAGTGAACAGGAAGGATGCCTTTCCGTGCCCATGAACTACCGGGCAGACGTGAAGCGCAT
GAGCAGGGTGCACCTGAGCGCCACCGACCTTGACGGCAATGCCATTGAGGAAGATCTGGAAGACTTTGACGCCATTGTCA
TGCAGCACGAATACGATCATCTTGACGGCATACTCTTTATTGACAAGGTCAGCCGCCTGCGGCGCAGCCTGTACGACAGC
AAGGTAAAAAAATGGCTCAAGAGAAAGACTGCCGTATAG

Upstream 100 bases:

>100_bases
AGCAGCCCGGCGAAAAAGGCAAGACCGACAAGCCCGGCGAGGCGGAAAAGGCGGAATAAAATCCGCCCGGCCGGAAGGCC
TTGAGCACAAGGAAGCAGCT

Downstream 100 bases:

>100_bases
TGTTTATGGGTACTCCCGGTTTTGCGGCCGCCAGTCTGAGGAGACTGGCGGCCTGGCCGCGTGCCCATATTGTCGCTGTA
TATACGCAGCCTGACCGTCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MILDIVTYPDPRLKEVCEPVAEVTDEIRQLAADMLETMYEAPGVGLAAPQVGRNIRMLVMDPSAQKDERNPRVLINPVLT
LSGEEIISEQEGCLSVPMNYRADVKRMSRVHLSATDLDGNAIEEDLEDFDAIVMQHEYDHLDGILFIDKVSRLRRSLYDS
KVKKWLKRKTAV

Sequences:

>Translated_172_residues
MILDIVTYPDPRLKEVCEPVAEVTDEIRQLAADMLETMYEAPGVGLAAPQVGRNIRMLVMDPSAQKDERNPRVLINPVLT
LSGEEIISEQEGCLSVPMNYRADVKRMSRVHLSATDLDGNAIEEDLEDFDAIVMQHEYDHLDGILFIDKVSRLRRSLYDS
KVKKWLKRKTAV
>Mature_172_residues
MILDIVTYPDPRLKEVCEPVAEVTDEIRQLAADMLETMYEAPGVGLAAPQVGRNIRMLVMDPSAQKDERNPRVLINPVLT
LSGEEIISEQEGCLSVPMNYRADVKRMSRVHLSATDLDGNAIEEDLEDFDAIVMQHEYDHLDGILFIDKVSRLRRSLYDS
KVKKWLKRKTAV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=172, Percent_Identity=32.5581395348837, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=45.4545454545455, Blast_Score=120, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24645726, Length=144, Percent_Identity=34.0277777777778, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=32.2784810126582, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19540; Mature: 19540

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDIVTYPDPRLKEVCEPVAEVTDEIRQLAADMLETMYEAPGVGLAAPQVGRNIRMLVM
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
DPSAQKDERNPRVLINPVLTLSGEEIISEQEGCLSVPMNYRADVKRMSRVHLSATDLDGN
CCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCH
AIEEDLEDFDAIVMQHEYDHLDGILFIDKVSRLRRSLYDSKVKKWLKRKTAV
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILDIVTYPDPRLKEVCEPVAEVTDEIRQLAADMLETMYEAPGVGLAAPQVGRNIRMLVM
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
DPSAQKDERNPRVLINPVLTLSGEEIISEQEGCLSVPMNYRADVKRMSRVHLSATDLDGN
CCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCH
AIEEDLEDFDAIVMQHEYDHLDGILFIDKVSRLRRSLYDSKVKKWLKRKTAV
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA