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The map label for this gene is nuoM [H]

Identifier: 218906468

GI number: 218906468

Start: 5083679

End: 5085181

Strand: Reverse

Name: nuoM [H]

Synonym: BCAH820_5382

Alternate gene names: 218906468

Gene position: 5085181-5083679 (Counterclockwise)

Preceding gene: 218906469

Following gene: 218906467

Centisome position: 95.9

GC content: 41.65

Gene sequence:

>1503_bases
ATGAATGATTTGTTATTAACGTTCTTCATTTTTTCCCCGCTTTTAGGAATTCTCTTACTTGCATTAACACCGAAAAAAGA
ATCGCGTACAGTGCGAGCGCTCGGTTTATTTGGAACGGTGCTTCCATTTGGAATGGCTATCGTCCTCGCTTGTACATATG
CTTCTGGAAGGGATTTGTCTCTATTTGATGAAAAAGTGAAATGGATTAAATTCGGGGATTTTGCAGCGGTAGATAAAAGA
TGGTTTTCCATTTATTACGAACTTGGTATAGATGGTTTATCGCTCGTTATGATGGTGCTGACGGCCCTTCTAGCGATGCT
TGCAGCAATTGCGGCATTTACGATTAAAAGAAATTTGAAAGCATTTTATATGCTGTTACTCATGTTAGAAATAGGGATGC
TCGGCGTCTTCGCTGCTCAAAATTTAATGTTGTTCTTTATCTTTTTTGAAATTACACTGCCACCGATGTTTTTATTAATT
GGGAAGTGGGGGAAATTGTCGAGTGAAAAGGCTGCTTATAGTTATTTAATATATAACGGTATTGGCTCCGCTATTTTACT
CATCGTTTTCTCGGTTTTATTTGCGAAAACAGGTACAACAAATATTACAGAACTGAAAGAAATATTAGCGAGTGTAGGTG
CTGGGGGAGGAATGGTTGTCCCAAGTAGCTTACAGTTCGGCTTGTTTCTTGCCATAATGATTGCTTTCGCAATTAAATTA
CCCGTTTTCCCGTTACATCGCTGGATGGTCAATGTACATATTGAAGCGCATCCGGCTGTAGTAATGCTTCATGCAGGTGT
TTTACTGAAGATTGGAGCGTACGGTATAATTCGCTTCGGGCAGGGGCTATTCCCGGAATACTTTCGCGAGTTTGCAACGC
TCATTGCGATTTTAGGAGTTATCAATTTATTGTACGGAGCCTTCTTAGCGCTCATTCAAACAGACTTTCGGAAGGTGCTT
GCTTATTCTAGTATTTCGCATATGGGTATTGTATTAATGGGCCTTGCGGCGTTAAATGCACCAGGTACACAAGGGGCACT
ATTCCAAGTTGTGTCGCATGGCTTAATTGCAGCCTTACTCTTTTTCTTACTTGGTGTTATAGAACAGCGTTTTGGAACGT
CGGATATTACAGCGCTTGGCGGACTTGCAAAAAGTGTACCAGTACTTAGCGGTTTCTTCTTAGCGGGAGGAATGGCATCG
CTTGGATTGCCGGGAATGTCTGGATTTGTTAGCGAGTTTCTTGCCTTTCTCGGTTTATTCCAAGGAGAGCCAGTCATTGC
TGCGGCCGGAGTACTTGGCATCATTTTAACCGCTGTATACGTATTAAGAGCAACACTGCAAGTAACATTTGGTAAGAAAG
AGTGGGAAGCGAAAGCTGATATACACGGATGGGAGTATGTTCCTATCTTGCTACTTATCTTCTGCATTATTGCAATTGGC
GTAATGCCAGAAATACTAGGGGATCCGCTTCAAAATACATTGAAAACATTGGGGGTGAAGTAG

Upstream 100 bases:

>100_bases
CTAGACTCCAAAACGGAAATGTACAAGTGTACGGGACTGTAACAGCTGTTTCACTTGCGGTACTCTTCATTATTTTGCTC
TATACGGGAGGGGATTTACG

Downstream 100 bases:

>100_bases
GATGGATATGAATACGTTACTTAGCTTATCGTGGCATCTCATGGTGCCAGAATTCATCATTCTCGGGGCTGCCATCCTTC
TTTCCATATGTGATTTGTTT

Product: NADH dehydrogenase subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK

Sequences:

>Translated_500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK
>Mature_500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI251831117, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788613, Length=463, Percent_Identity=35.4211663066955, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788614, Length=512, Percent_Identity=28.3203125, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=28.525641025641, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=27.6353276353276, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788829, Length=428, Percent_Identity=23.1308411214953, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI2367154, Length=352, Percent_Identity=25.8522727272727, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI145693160, Length=403, Percent_Identity=24.8138957816377, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54080; Mature: 54080

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LFDEKVKWIKFGDFAAVDKRWFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKWGKLSSEKAAYSYLIYNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHC
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGV
CCHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
INLLYGAFLALIQTDFRKVLAYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMASLGLPGMSGFVSEFLAFLGLF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHC
QGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VMPEILGDPLQNTLKTLGVK
HHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]