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The map label for this gene is hisF [H]

Identifier: 218902618

GI number: 218902618

Start: 1419250

End: 1420008

Strand: Direct

Name: hisF [H]

Synonym: BCAH820_1501

Alternate gene names: 218902618

Gene position: 1419250-1420008 (Clockwise)

Preceding gene: 218902617

Following gene: 218902619

Centisome position: 26.76

GC content: 40.45

Gene sequence:

>759_bases
ATGTTAGCGAAACGCATTATTCCATGTCTAGATGTGAAAGAAGGGCGAGTCGTAAAGGGGGTAAATTTTATAGGGTTACA
AGATGTCGGTGATCCTGTTGAAATAGCAGCTTTATATAATGATGCGGGAGCAGATGAAATAGTATTTTTAGATATTACGG
CAACGCATGAAGGGCGAAAAACAATTATAGATGTTGTAGAAAAAACGGCTTCAAAAGTATTTATTCCTCTTACAGTTGGC
GGAGGAATTTCAAGTGTGAAAGATATGTACAATTTACTAAGAGCGGGAGCAGATAAAGTTTCAATTAACTCAGCAGCAGT
GCGAAATCCAAAATTAATCGAAGAAGGGGCACAGCACTTTGGTTCACAATGTATTGTCGTAGCAATTGATGCTAGAAAAG
TAGCAGAAGGTAAGTGGAATGTATATGTGAACGGCGGAAGAGTTGATACGGGAATGGATGCAATTGAGTGGGCAAAGCGC
GTTGTTATGCTTGGTGCCGGCGAAATTTTGTTAACGAGTATGGATGCAGATGGAACGAAAAACGGATATGACCTTCGTTT
AACAGAAGAAATTTCAAAAAGTGTTTCCGTACCAGTCATTGCGTCGGGTGGATGTGGTCATGCTGATCACATTATAGAAG
TGTTTCAAAAGACAACAGTAGATGCAGCGTTAGCGGCATCAATCTTTCATTACGGTGAAGTGACAATAGGGGACGTAAAG
AGAAAGTTAAGAAATGCAAACGTCGAGGTGCGGTTATGA

Upstream 100 bases:

>100_bases
TGAAAAAGTTAAATGATATGAACATATACGGCGTCATAATTGGTAAGGCTCTTTACGAGAAAACGATTGATTTAGAAGAA
GTGCTAGAGGTAACAAAGTT

Downstream 100 bases:

>100_bases
AACCTAACTTTTCAAAAGGACTATTACCGGCAGTTGTAATTGAAGAAGGTACAAAAGAAGTTTTAATGCTAGCTTATATG
AATGAAGAAGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEVTIGDVK
RKLRNANVEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEVTIGDVK
RKLRNANVEVRL
>Mature_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKR
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEVTIGDVK
RKLRNANVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.8992248062016, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=27.7551020408163, Blast_Score=90, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.0764331210191, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26960; Mature: 26960

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKRVVMLGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHEEEEECCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEVTIGDVK
CCEEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKRVVMLGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHHEEEEECCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEVTIGDVK
CCEEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA