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The map label for this gene is top6B [H]

Identifier: 218884530

GI number: 218884530

Start: 1141616

End: 1143208

Strand: Reverse

Name: top6B [H]

Synonym: DKAM_1219

Alternate gene names: 218884530

Gene position: 1143208-1141616 (Counterclockwise)

Preceding gene: 218884533

Following gene: 218884529

Centisome position: 83.74

GC content: 46.83

Gene sequence:

>1593_bases
ATGGGTGAGACCCCGGTAGAGAAATACAGGGCTATTAGTCCAGCCGAGTTCTTCTACAAGTACCGTGAGATAGCTGGATT
CTCCAATCCAGCTAGAGCTATTTATCAGACTGTCAGAGAGCTAGTTGAGAACGCCCTCGACGCCACGGATGCGCACGGTA
TAATGCCTGATATAAAAATCTCTATTAGGAAGGTGGATGAGATACAAGAATTCTATAGGATCACTGTTGAAGACAACGGT
ATAGGAATACCTCCAGACGTAGTTCCAGATGCCTTCGGCAGGGTGTTATTCAGTAGTAAATATGTGTTAAGGCAGACACG
TGGAATGTACGGTCTTGGAGTGAAGATGGCTGTCCTCTATGGGCAGATGACCACTGGGAGACCTGTAGAGGTGATCACCA
GTAAACCGGGTTTTAAGAGGATATACTACTTCAAGCTCAGGATAGATGTTAATAGGAATGAGCCAGTGATAATTGAAAGG
GGCTCCTGGAGGAAGAACAGGGAGTGGCATGGAACCATAGTATCTATAACCATAGAGGGGGATTGGGGTAGGGCGAAGCC
AAAGATTAAGGAATACTTGTTTAAGACGGCTATAGTGACTCCCTATGCCAACATAGTGGCGTTGACCCCTGATGATGAGA
TAATATACTATCCGAGGATAATAGATAAAATACCCAGGCCACCTCTCGAGGTCAAGCCACATCCCCACGGCGTAGACCTC
GAGCTCTTGAAGCAGATTATTTCGAGCCATAACTACACTAGTGTTGAGGAACTACTCATCAAGGGATTCCAGAGCATTGG
GGAGGCAACAGCTAAAAGCATCCTGCAGCAGGCCGGGATAGACCCTGGTAAACCCATCTCAGAGCTCTCGGATAACGAGT
TGCTATCGCTGGTTAACACGCTTAAGTCCATGAAGGATATCAGGGCTCCCTCGATAAAGGCTATTTCACCTCTTGGAAGC
GATGTAATTATAGCTGGCTTGAAACGCGTATTCCAACCCGAGTTCGTCTATGCTGTCTCAAGACGTCCACAGGCATATCA
GGGACATCCCTTCATTGTCGAGGCCGGTATAGCCTATGGCGGTAAGACGCCGATGAGCGATGATGATAAGCCAATAATAC
TTAGATACGCGAATAAGATTCCATTGCTCTATGATGAGGGAAGCGATGTCACAACGATGGTTGTCAAGGAGGATATAAGT
TGGGACCACTACATGGTCAGCTTCCCAGCCCCCATAGCAATACTTGTACATGTGTGCAGTACAAAAATACCCTTCAAGGG
TGTTGGGAAGGAGAGCATAGCAGATGTCCCCGAGGTTAGGAAGGAGATAAAGCTAGTACTCATGGACGTGCTCAGAGAGC
TTAAGAAGTATCTTTCAAAGAAGGCTAAAGAAGAGGAGTTGAAGAAGAGGGCTGAGCATCTGACAAAGTATATCCCGGAG
GTGGCTAGAGGGCTCTCGGTGATAGTGGCGAATGGTAGTAAGGACTTCGAGGTGAAGCTGGTTCAAAAGCTGGTCTCAAT
AATATCTAGGCGTACAGGTATACCTGAATCCGAGATACAGCAGGTACTTGAATCAATTAGAATAGGGACTTAG

Upstream 100 bases:

>100_bases
CCCGGCAACAGGGAGCATAGAGTCCTAAACCACTTGTTTTTAAGTAATTTATTGAATCATAAAACTAGTGGTTTCGGAAA
CGTTTTCCAGGTGTTTCACT

Downstream 100 bases:

>100_bases
GTGATTGTATAATGGTGTCCGCAATCGATCTTGAGGCGAGGAAGAAGGCGGCTGAGTTAATGAGGAAAATATTTATGAAT
ATAGTAAGTGAGATAGAAAG

Product: DNA topoisomerase VI subunit B

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit B; TopoVI-B [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MGETPVEKYRAISPAEFFYKYREIAGFSNPARAIYQTVRELVENALDATDAHGIMPDIKISIRKVDEIQEFYRITVEDNG
IGIPPDVVPDAFGRVLFSSKYVLRQTRGMYGLGVKMAVLYGQMTTGRPVEVITSKPGFKRIYYFKLRIDVNRNEPVIIER
GSWRKNREWHGTIVSITIEGDWGRAKPKIKEYLFKTAIVTPYANIVALTPDDEIIYYPRIIDKIPRPPLEVKPHPHGVDL
ELLKQIISSHNYTSVEELLIKGFQSIGEATAKSILQQAGIDPGKPISELSDNELLSLVNTLKSMKDIRAPSIKAISPLGS
DVIIAGLKRVFQPEFVYAVSRRPQAYQGHPFIVEAGIAYGGKTPMSDDDKPIILRYANKIPLLYDEGSDVTTMVVKEDIS
WDHYMVSFPAPIAILVHVCSTKIPFKGVGKESIADVPEVRKEIKLVLMDVLRELKKYLSKKAKEEELKKRAEHLTKYIPE
VARGLSVIVANGSKDFEVKLVQKLVSIISRRTGIPESEIQQVLESIRIGT

Sequences:

>Translated_530_residues
MGETPVEKYRAISPAEFFYKYREIAGFSNPARAIYQTVRELVENALDATDAHGIMPDIKISIRKVDEIQEFYRITVEDNG
IGIPPDVVPDAFGRVLFSSKYVLRQTRGMYGLGVKMAVLYGQMTTGRPVEVITSKPGFKRIYYFKLRIDVNRNEPVIIER
GSWRKNREWHGTIVSITIEGDWGRAKPKIKEYLFKTAIVTPYANIVALTPDDEIIYYPRIIDKIPRPPLEVKPHPHGVDL
ELLKQIISSHNYTSVEELLIKGFQSIGEATAKSILQQAGIDPGKPISELSDNELLSLVNTLKSMKDIRAPSIKAISPLGS
DVIIAGLKRVFQPEFVYAVSRRPQAYQGHPFIVEAGIAYGGKTPMSDDDKPIILRYANKIPLLYDEGSDVTTMVVKEDIS
WDHYMVSFPAPIAILVHVCSTKIPFKGVGKESIADVPEVRKEIKLVLMDVLRELKKYLSKKAKEEELKKRAEHLTKYIPE
VARGLSVIVANGSKDFEVKLVQKLVSIISRRTGIPESEIQQVLESIRIGT
>Mature_529_residues
GETPVEKYRAISPAEFFYKYREIAGFSNPARAIYQTVRELVENALDATDAHGIMPDIKISIRKVDEIQEFYRITVEDNGI
GIPPDVVPDAFGRVLFSSKYVLRQTRGMYGLGVKMAVLYGQMTTGRPVEVITSKPGFKRIYYFKLRIDVNRNEPVIIERG
SWRKNREWHGTIVSITIEGDWGRAKPKIKEYLFKTAIVTPYANIVALTPDDEIIYYPRIIDKIPRPPLEVKPHPHGVDLE
LLKQIISSHNYTSVEELLIKGFQSIGEATAKSILQQAGIDPGKPISELSDNELLSLVNTLKSMKDIRAPSIKAISPLGSD
VIIAGLKRVFQPEFVYAVSRRPQAYQGHPFIVEAGIAYGGKTPMSDDDKPIILRYANKIPLLYDEGSDVTTMVVKEDISW
DHYMVSFPAPIAILVHVCSTKIPFKGVGKESIADVPEVRKEIKLVLMDVLRELKKYLSKKAKEEELKKRAEHLTKYIPEV
ARGLSVIVANGSKDFEVKLVQKLVSIISRRTGIPESEIQQVLESIRIGT

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP [H]

COG id: COG1389

COG function: function code L; DNA topoisomerase VI, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008616
- InterPro:   IPR010979
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005734
- InterPro:   IPR015320
- ProDom:   PD013790 [H]

Pfam domain/function: PF05833 FbpA; PF02518 HATPase_c; PF09239 Topo-VIb_trans [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 59661; Mature: 59530

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETPVEKYRAISPAEFFYKYREIAGFSNPARAIYQTVRELVENALDATDAHGIMPDIKI
CCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
SIRKVDEIQEFYRITVEDNGIGIPPDVVPDAFGRVLFSSKYVLRQTRGMYGLGVKMAVLY
HHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHC
GQMTTGRPVEVITSKPGFKRIYYFKLRIDVNRNEPVIIERGSWRKNREWHGTIVSITIEG
CCCCCCCCEEEEECCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEC
DWGRAKPKIKEYLFKTAIVTPYANIVALTPDDEIIYYPRIIDKIPRPPLEVKPHPHGVDL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHCCCCCCCCCCCCCCCCH
ELLKQIISSHNYTSVEELLIKGFQSIGEATAKSILQQAGIDPGKPISELSDNELLSLVNT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHH
LKSMKDIRAPSIKAISPLGSDVIIAGLKRVFQPEFVYAVSRRPQAYQGHPFIVEAGIAYG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCC
GKTPMSDDDKPIILRYANKIPLLYDEGSDVTTMVVKEDISWDHYMVSFPAPIAILVHVCS
CCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHC
TKIPFKGVGKESIADVPEVRKEIKLVLMDVLRELKKYLSKKAKEEELKKRAEHLTKYIPE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARGLSVIVANGSKDFEVKLVQKLVSIISRRTGIPESEIQQVLESIRIGT
HHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GETPVEKYRAISPAEFFYKYREIAGFSNPARAIYQTVRELVENALDATDAHGIMPDIKI
CCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
SIRKVDEIQEFYRITVEDNGIGIPPDVVPDAFGRVLFSSKYVLRQTRGMYGLGVKMAVLY
HHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHC
GQMTTGRPVEVITSKPGFKRIYYFKLRIDVNRNEPVIIERGSWRKNREWHGTIVSITIEG
CCCCCCCCEEEEECCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEC
DWGRAKPKIKEYLFKTAIVTPYANIVALTPDDEIIYYPRIIDKIPRPPLEVKPHPHGVDL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHCCCCCCCCCCCCCCCCH
ELLKQIISSHNYTSVEELLIKGFQSIGEATAKSILQQAGIDPGKPISELSDNELLSLVNT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHH
LKSMKDIRAPSIKAISPLGSDVIIAGLKRVFQPEFVYAVSRRPQAYQGHPFIVEAGIAYG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCC
GKTPMSDDDKPIILRYANKIPLLYDEGSDVTTMVVKEDISWDHYMVSFPAPIAILVHVCS
CCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHC
TKIPFKGVGKESIADVPEVRKEIKLVLMDVLRELKKYLSKKAKEEELKKRAEHLTKYIPE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARGLSVIVANGSKDFEVKLVQKLVSIISRRTGIPESEIQQVLESIRIGT
HHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA