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The map label for this gene is cysH [C]

Identifier: 218884285

GI number: 218884285

Start: 919828

End: 921132

Strand: Reverse

Name: cysH [C]

Synonym: DKAM_0974

Alternate gene names: 218884285

Gene position: 921132-919828 (Counterclockwise)

Preceding gene: 218884288

Following gene: 218884277

Centisome position: 67.47

GC content: 45.21

Gene sequence:

>1305_bases
GTGGTATTCATGGGTAACGACGTCATCATTGATGAGGGATGGGTGTGCTGGGATAGCAAGAGGAATGCTCCCAGATTAAA
CTGTAGGAAATGGAGGATGAGGCATGATTACTGGCTGGCGGGTTTATTCGAGAAGAGGCTTGTAGAGGAATTAATCTCAG
CCGAGTTCAAGATCCATTACCGTCTTCAAGATAGAAACGTACTCATCCACCGTGCACCTAATCCCTCGTCACGGAGCATG
TTAGCACTAGAGGTATTCGCTGATGGTGTGAGACTGGGTGTAGTGGAGTATAGGATGGGGGAAAGATGGATGATCCATCC
TAGCGGTGGGTTAGCTAGTCTACTCAACAGTCTAGGTGCATACAGTGTTGAGGTGGAGCCCGGCCATGGGAGAAAGCTGA
AAGGCAAAAAAGTGAAGGTAGTTGGGGATATAGATGGTCTGCGATGGATTCTATTAGACTTAGACAAGTATATTGGTGTT
GCAAGGGTTATTGATGCATCGAAGAAGATTGTCAAAGTAAAGGATGTAGCACCGAAAGGGTTCAAGCCCCTCCCAGCTAC
AGGTATTATACAGGTTGTAGAAGCTAATAGAAGCCTGCTTGAGACAGCTGTCAACGAAGCTGTGGGCTTCCTGAAGAAAA
CACTAGAGAATGTTATGGATGGTATAGCGGTCTCCTATAGTGGTGGGGCTGATTCAACAGCTTCACTAGTTTTATCTCAA
GAGGCATTAGGCCATGCAAGGATAAAGGTGGTCTATGTAGATACAGGCATGGATCTACCCGGTGTCAAAGAATATGCTCT
AAGAATACTTGATAAACTCGGCTTAATACCCTATGTAGTGTCTTCGGGATTGGATCCACTCAATGAGATTGCTTCTCGTG
GACTACCCACGCGCGATAACAGGTGGTGTACGAGAATACTGAAACTTGAACCCTTAAGGAGATTCTACAGGGAACACAAT
ATAAGATTCGTTATCGACGGATCTAGAAGCAGGGAAAGCAGTAGTCGAGCCGAGACGCCGAGGATAGGTAGAAATCCCTT
AATACCTGAAGTCACAAGGATATTACCGATCAAGTGGTGGAGTAGGTTACTGGTTCAATTATACATAGTGAACAGGGGAA
TAGAGTTAAACCCCTTGTACAATGAAGGCTTCACCAGGCTAGGCTGTATACTGTGTCCTGCAATGCATCCGCACGAACTT
GAATTATCCTACACCAAAAATAGATACTGGTTTGAGAGATTACGGGAGGTCACGGGTCTCTCGATAAACGACGTAATGAT
GTATCTTTACAGTGAGAAACAATAA

Upstream 100 bases:

>100_bases
TATGATCTGGGCGAAATCTATGATTGCTGTGGGGATCCCCCCAGCTTTAAATACTGTGTAGAGATCGCTACTATACCCTG
ATAGTGGAAGCTGCGTAGAA

Downstream 100 bases:

>100_bases
TTGATTATGGGCCCGGCGGGATTTGAACCCGCGTCCTCCCGGTTATCAGCCGGGCGCTCCACCAAGCTGAGCTACGGGCC
CCCAGGTATCCACAACATGT

Product: phosphoadenosine phosphosulfate reductase

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MVFMGNDVIIDEGWVCWDSKRNAPRLNCRKWRMRHDYWLAGLFEKRLVEELISAEFKIHYRLQDRNVLIHRAPNPSSRSM
LALEVFADGVRLGVVEYRMGERWMIHPSGGLASLLNSLGAYSVEVEPGHGRKLKGKKVKVVGDIDGLRWILLDLDKYIGV
ARVIDASKKIVKVKDVAPKGFKPLPATGIIQVVEANRSLLETAVNEAVGFLKKTLENVMDGIAVSYSGGADSTASLVLSQ
EALGHARIKVVYVDTGMDLPGVKEYALRILDKLGLIPYVVSSGLDPLNEIASRGLPTRDNRWCTRILKLEPLRRFYREHN
IRFVIDGSRSRESSSRAETPRIGRNPLIPEVTRILPIKWWSRLLVQLYIVNRGIELNPLYNEGFTRLGCILCPAMHPHEL
ELSYTKNRYWFERLREVTGLSINDVMMYLYSEKQ

Sequences:

>Translated_434_residues
MVFMGNDVIIDEGWVCWDSKRNAPRLNCRKWRMRHDYWLAGLFEKRLVEELISAEFKIHYRLQDRNVLIHRAPNPSSRSM
LALEVFADGVRLGVVEYRMGERWMIHPSGGLASLLNSLGAYSVEVEPGHGRKLKGKKVKVVGDIDGLRWILLDLDKYIGV
ARVIDASKKIVKVKDVAPKGFKPLPATGIIQVVEANRSLLETAVNEAVGFLKKTLENVMDGIAVSYSGGADSTASLVLSQ
EALGHARIKVVYVDTGMDLPGVKEYALRILDKLGLIPYVVSSGLDPLNEIASRGLPTRDNRWCTRILKLEPLRRFYREHN
IRFVIDGSRSRESSSRAETPRIGRNPLIPEVTRILPIKWWSRLLVQLYIVNRGIELNPLYNEGFTRLGCILCPAMHPHEL
ELSYTKNRYWFERLREVTGLSINDVMMYLYSEKQ
>Mature_434_residues
MVFMGNDVIIDEGWVCWDSKRNAPRLNCRKWRMRHDYWLAGLFEKRLVEELISAEFKIHYRLQDRNVLIHRAPNPSSRSM
LALEVFADGVRLGVVEYRMGERWMIHPSGGLASLLNSLGAYSVEVEPGHGRKLKGKKVKVVGDIDGLRWILLDLDKYIGV
ARVIDASKKIVKVKDVAPKGFKPLPATGIIQVVEANRSLLETAVNEAVGFLKKTLENVMDGIAVSYSGGADSTASLVLSQ
EALGHARIKVVYVDTGMDLPGVKEYALRILDKLGLIPYVVSSGLDPLNEIASRGLPTRDNRWCTRILKLEPLRRFYREHN
IRFVIDGSRSRESSSRAETPRIGRNPLIPEVTRILPIKWWSRLLVQLYIVNRGIELNPLYNEGFTRLGCILCPAMHPHEL
ELSYTKNRYWFERLREVTGLSINDVMMYLYSEKQ

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014729
- InterPro:   IPR004521 [H]

Pfam domain/function: PF01507 PAPS_reduct; PF01472 PUA [H]

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 49423; Mature: 49423

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFMGNDVIIDEGWVCWDSKRNAPRLNCRKWRMRHDYWLAGLFEKRLVEELISAEFKIHY
CEEECCEEEEECCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
RLQDRNVLIHRAPNPSSRSMLALEVFADGVRLGVVEYRMGERWMIHPSGGLASLLNSLGA
EEECCCEEEEECCCCCCCCEEEEEEHHCCEEEEEEEEECCCEEEEECCCCHHHHHHHCCC
YSVEVEPGHGRKLKGKKVKVVGDIDGLRWILLDLDKYIGVARVIDASKKIVKVKDVAPKG
EEEEECCCCCCCCCCCEEEEEECCCCCEEHEEEHHHHHHHHHHHHCCCCEEEEECCCCCC
FKPLPATGIIQVVEANRSLLETAVNEAVGFLKKTLENVMDGIAVSYSGGADSTASLVLSQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHEEEH
EALGHARIKVVYVDTGMDLPGVKEYALRILDKLGLIPYVVSSGLDPLNEIASRGLPTRDN
HHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHCCCCCCCC
RWCTRILKLEPLRRFYREHNIRFVIDGSRSRESSSRAETPRIGRNPLIPEVTRILPIKWW
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SRLLVQLYIVNRGIELNPLYNEGFTRLGCILCPAMHPHELELSYTKNRYWFERLREVTGL
HHHHHHHHHHHCCCEECCCHHCCHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCC
SINDVMMYLYSEKQ
CHHHHHEEEECCCC
>Mature Secondary Structure
MVFMGNDVIIDEGWVCWDSKRNAPRLNCRKWRMRHDYWLAGLFEKRLVEELISAEFKIHY
CEEECCEEEEECCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
RLQDRNVLIHRAPNPSSRSMLALEVFADGVRLGVVEYRMGERWMIHPSGGLASLLNSLGA
EEECCCEEEEECCCCCCCCEEEEEEHHCCEEEEEEEEECCCEEEEECCCCHHHHHHHCCC
YSVEVEPGHGRKLKGKKVKVVGDIDGLRWILLDLDKYIGVARVIDASKKIVKVKDVAPKG
EEEEECCCCCCCCCCCEEEEEECCCCCEEHEEEHHHHHHHHHHHHCCCCEEEEECCCCCC
FKPLPATGIIQVVEANRSLLETAVNEAVGFLKKTLENVMDGIAVSYSGGADSTASLVLSQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHEEEH
EALGHARIKVVYVDTGMDLPGVKEYALRILDKLGLIPYVVSSGLDPLNEIASRGLPTRDN
HHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHCCCCCCCC
RWCTRILKLEPLRRFYREHNIRFVIDGSRSRESSSRAETPRIGRNPLIPEVTRILPIKWW
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SRLLVQLYIVNRGIELNPLYNEGFTRLGCILCPAMHPHELELSYTKNRYWFERLREVTGL
HHHHHHHHHHHCCCEECCCHHCCHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCC
SINDVMMYLYSEKQ
CHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]