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The map label for this gene is nfi

Identifier: 218884175

GI number: 218884175

Start: 829070

End: 829729

Strand: Direct

Name: nfi

Synonym: DKAM_0864

Alternate gene names: 218884175

Gene position: 829070-829729 (Clockwise)

Preceding gene: 218884172

Following gene: 218884179

Centisome position: 60.73

GC content: 48.03

Gene sequence:

>660_bases
ATGGGCTTCGATTATCAGCGGGCTGTAATGCTTCAGAGAATACTCTCTGAGAGGGTTCTAGCAGAGCTTGATTCATTTCC
AAGGATTGATCCATCGAGAATAAGGAGTGTTGCAGGGGTTGATGCATCATATAGGGGAGGAGTTCAGGTTGGAAGCGCTG
TACTCATGGATTATCGGGCTAAGATGCCCCTGGCATATACGTGTCTTACATCGAAGCCCCCTATACCATATGTGCCAGGT
CTACTGGCGTTCAGGGAGGCCCCTGTATACATTAAGGCATTGCATAGGTTGCCGGCTAAACCGGATATAATATTGGTTGA
TGGACACGGATTAAGCCATCCAAGAGCATTTGGCATAGCAACACATATAGGTCTCGTACTCAGCACCCCGTCAATAGGGG
TGGCAAAGAAGCCGCTCTACGGGGAGGTAGAAGAAGTAAATGGTAGGAAACTCGTGAGGGCGCATGGAAGAATAGTGGGT
GAGGTAGTTGAAACCAACCAGGGCAGCGAAATATATGTGAGTATAGGATACCTAATAAGGCTTGAAGACGCGGTGGAAGT
AGTTAGACACCTCATGGAGCCAGGCCTAAAGCTACCACTACCAATACACCTGGCAGACAACTACTCCAGGAGTAAATGCA
TCAAGGAGCTACGGTTATAA

Upstream 100 bases:

>100_bases
TAAACCACCGTGGTAAAGACACCAAGAAGATAAAACACATCCAAAAATATTTAAAAAGAATTTAGAGCCTGCAACAGACT
AATTAAGTAAGGGTATTTGA

Downstream 100 bases:

>100_bases
CGTAGTGAAGATGTTATGAATTCATATCTACTTATCTAGGTACATGGACAAAAATCTCTACACTTATTCGCGATATGAGA
TATAAGCTCAGAGTTCAGGA

Product: Endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MGFDYQRAVMLQRILSERVLAELDSFPRIDPSRIRSVAGVDASYRGGVQVGSAVLMDYRAKMPLAYTCLTSKPPIPYVPG
LLAFREAPVYIKALHRLPAKPDIILVDGHGLSHPRAFGIATHIGLVLSTPSIGVAKKPLYGEVEEVNGRKLVRAHGRIVG
EVVETNQGSEIYVSIGYLIRLEDAVEVVRHLMEPGLKLPLPIHLADNYSRSKCIKELRL

Sequences:

>Translated_219_residues
MGFDYQRAVMLQRILSERVLAELDSFPRIDPSRIRSVAGVDASYRGGVQVGSAVLMDYRAKMPLAYTCLTSKPPIPYVPG
LLAFREAPVYIKALHRLPAKPDIILVDGHGLSHPRAFGIATHIGLVLSTPSIGVAKKPLYGEVEEVNGRKLVRAHGRIVG
EVVETNQGSEIYVSIGYLIRLEDAVEVVRHLMEPGLKLPLPIHLADNYSRSKCIKELRL
>Mature_218_residues
GFDYQRAVMLQRILSERVLAELDSFPRIDPSRIRSVAGVDASYRGGVQVGSAVLMDYRAKMPLAYTCLTSKPPIPYVPGL
LAFREAPVYIKALHRLPAKPDIILVDGHGLSHPRAFGIATHIGLVLSTPSIGVAKKPLYGEVEEVNGRKLVRAHGRIVGE
VVETNQGSEIYVSIGYLIRLEDAVEVVRHLMEPGLKLPLPIHLADNYSRSKCIKELRL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=222, Percent_Identity=31.0810810810811, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI87082357, Length=186, Percent_Identity=33.8709677419355, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_DESK1 (B8D509)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428557.1
- ProteinModelPortal:   B8D509
- GeneID:   7171004
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_0864
- HOGENOM:   HBG716360
- OMA:   SVGHRIG
- ProtClustDB:   CLSK965765
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 24171; Mature: 24040

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFDYQRAVMLQRILSERVLAELDSFPRIDPSRIRSVAGVDASYRGGVQVGSAVLMDYRA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
KMPLAYTCLTSKPPIPYVPGLLAFREAPVYIKALHRLPAKPDIILVDGHGLSHPRAFGIA
CCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
THIGLVLSTPSIGVAKKPLYGEVEEVNGRKLVRAHGRIVGEVVETNQGSEIYVSIGYLIR
HHHHHEEECCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
LEDAVEVVRHLMEPGLKLPLPIHLADNYSRSKCIKELRL
EHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
GFDYQRAVMLQRILSERVLAELDSFPRIDPSRIRSVAGVDASYRGGVQVGSAVLMDYRA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
KMPLAYTCLTSKPPIPYVPGLLAFREAPVYIKALHRLPAKPDIILVDGHGLSHPRAFGIA
CCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
THIGLVLSTPSIGVAKKPLYGEVEEVNGRKLVRAHGRIVGEVVETNQGSEIYVSIGYLIR
HHHHHEEECCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
LEDAVEVVRHLMEPGLKLPLPIHLADNYSRSKCIKELRL
EHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA