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The map label for this gene is cobB [H]

Identifier: 218710617

GI number: 218710617

Start: 2858223

End: 2859527

Strand: Direct

Name: cobB [H]

Synonym: VS_2671

Alternate gene names: 218710617

Gene position: 2858223-2859527 (Clockwise)

Preceding gene: 218710616

Following gene: 218710618

Centisome position: 86.63

GC content: 42.84

Gene sequence:

>1305_bases
ATGTCTACAGCTTACTGTTCTGCCCTAGTGGTAGGCGCACCTTCTTCTGGAAGCGGAAAAACAACCGTGGTCGCCGCTCT
AGCAAGAGCTTTGGTTCAACAAGGAAAGAAAGTTCGAGTATTCAAAACTGGGCCTGATTTTATTGATCCACAATTTCTAT
CTATTGCATCAGATACACCCACTTACCAATTGGATTTGTGGATGTGTGGTGAAGCGGAATGCCAACACTTAATTTACCAA
GCAGCCCTAGAAGCGGATGTGATTCTAATTGAAGGCGTAATGGGTATGTTTGATGGCAAATGCTCAAGTGCTGATATTGC
CGCTAAATTTAACCTTCCGATGTTGGCCGTGGTAGATGCTGGCGCAATGGCACAAACCTTTGGAGCTATTGTAAATGGCT
TATCGACATTCCGTGATGACGTGGATGTATTTGGTGTAGTTGCAAACCGCGTAGGTTCTGAACGTCACGCTGAAATGCTA
AAAGAAAGCCTAAAACCTGAAACCGAGTTTTGTGGTTGGTTACCAAAAGACATGGATTTGTCGCTGCCAGAACGCCACTT
AGGTTTAGTACAAGCACAAGAGTTAGATGACATAGAGGAGCGACTTAATCACGCAGCCAAGCTACTACTAAAACATGGTG
AGATCCCATTACCAACAGCACAAGCATTTGAAAAGCCAAAGCAAGCACTACCAGCCAGACATGAATCATTAACTGGTCAT
ACAATTGCAGTAGCTCGTGATAATAGCTTTGCTTTCTTGTATCAGGCTAACCTTGATTTGTTGATCGAATTGGGGGCAAA
ATTGTCATTTTTCTCACCACTTGCACATGATGATTTACCAGTGTGTGACGCTTTGTATTTACCAGGAGGTTATCCTGAAT
TACATATGGATAAGCTAGCGCTCAATCATGCGTTGAAGCAGCAAATCATCAAACACATTGAACAAGGAAAACCTTGCCTA
GCCGAATGTGGTGGCATGCTATACCTAAACAAAGCATTGACTGATACAGAAAGCCAAACAGAAAACATGGTTGGAGCATT
AAATGCCAAAGCGACTATGCAGAAGAAGCTAGCCGCGCTGGGGATCCTAGAAGCTAATTTCGATGGTCAAATGCTGCGTG
GGCATACTTTCCATTATTCAAAAACAGACAGTAAAGAAACCGTGCTAACTCAACCTGAATCTCAATATGGTAGGCCAACC
GATCCTGTTTGGATAAAACAAAGCACCATCGCTTCTTATGCACATTGGTACTTCCCGTATAACCCAGATTTGGTAGCGAA
AATATTCAAAGGAGAGTTTTATTAA

Upstream 100 bases:

>100_bases
GTCATTGTGATGAAAACGATGCACTTCGTTTCGGGTTACCCGCCAACGAATCACAATGGCAAGCGCTCACTCGCGCACTC
GAACAACTCAAGGAATAATT

Downstream 100 bases:

>100_bases
GCATCGACGAAAAAATACGCCTTGAACGCTTTCAATAGGGCGTGTTGATCTTTCGAGCTGATTTTTGCAGCGAGTTGCTG
GGTATTTATACAAGGCAGAA

Product: cobyrinic acid A,C-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSTAYCSALVVGAPSSGSGKTTVVAALARALVQQGKKVRVFKTGPDFIDPQFLSIASDTPTYQLDLWMCGEAECQHLIYQ
AALEADVILIEGVMGMFDGKCSSADIAAKFNLPMLAVVDAGAMAQTFGAIVNGLSTFRDDVDVFGVVANRVGSERHAEML
KESLKPETEFCGWLPKDMDLSLPERHLGLVQAQELDDIEERLNHAAKLLLKHGEIPLPTAQAFEKPKQALPARHESLTGH
TIAVARDNSFAFLYQANLDLLIELGAKLSFFSPLAHDDLPVCDALYLPGGYPELHMDKLALNHALKQQIIKHIEQGKPCL
AECGGMLYLNKALTDTESQTENMVGALNAKATMQKKLAALGILEANFDGQMLRGHTFHYSKTDSKETVLTQPESQYGRPT
DPVWIKQSTIASYAHWYFPYNPDLVAKIFKGEFY

Sequences:

>Translated_434_residues
MSTAYCSALVVGAPSSGSGKTTVVAALARALVQQGKKVRVFKTGPDFIDPQFLSIASDTPTYQLDLWMCGEAECQHLIYQ
AALEADVILIEGVMGMFDGKCSSADIAAKFNLPMLAVVDAGAMAQTFGAIVNGLSTFRDDVDVFGVVANRVGSERHAEML
KESLKPETEFCGWLPKDMDLSLPERHLGLVQAQELDDIEERLNHAAKLLLKHGEIPLPTAQAFEKPKQALPARHESLTGH
TIAVARDNSFAFLYQANLDLLIELGAKLSFFSPLAHDDLPVCDALYLPGGYPELHMDKLALNHALKQQIIKHIEQGKPCL
AECGGMLYLNKALTDTESQTENMVGALNAKATMQKKLAALGILEANFDGQMLRGHTFHYSKTDSKETVLTQPESQYGRPT
DPVWIKQSTIASYAHWYFPYNPDLVAKIFKGEFY
>Mature_433_residues
STAYCSALVVGAPSSGSGKTTVVAALARALVQQGKKVRVFKTGPDFIDPQFLSIASDTPTYQLDLWMCGEAECQHLIYQA
ALEADVILIEGVMGMFDGKCSSADIAAKFNLPMLAVVDAGAMAQTFGAIVNGLSTFRDDVDVFGVVANRVGSERHAEMLK
ESLKPETEFCGWLPKDMDLSLPERHLGLVQAQELDDIEERLNHAAKLLLKHGEIPLPTAQAFEKPKQALPARHESLTGHT
IAVARDNSFAFLYQANLDLLIELGAKLSFFSPLAHDDLPVCDALYLPGGYPELHMDKLALNHALKQQIIKHIEQGKPCLA
ECGGMLYLNKALTDTESQTENMVGALNAKATMQKKLAALGILEANFDGQMLRGHTFHYSKTDSKETVLTQPESQYGRPTD
PVWIKQSTIASYAHWYFPYNPDLVAKIFKGEFY

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47578; Mature: 47447

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAYCSALVVGAPSSGSGKTTVVAALARALVQQGKKVRVFKTGPDFIDPQFLSIASDTP
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEECCCCC
TYQLDLWMCGEAECQHLIYQAALEADVILIEGVMGMFDGKCSSADIAAKFNLPMLAVVDA
CEEEEEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCEEEECCCCEEEEECC
GAMAQTFGAIVNGLSTFRDDVDVFGVVANRVGSERHAEMLKESLKPETEFCGWLPKDMDL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCC
SLPERHLGLVQAQELDDIEERLNHAAKLLLKHGEIPLPTAQAFEKPKQALPARHESLTGH
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCCHHHCCCCC
TIAVARDNSFAFLYQANLDLLIELGAKLSFFSPLAHDDLPVCDALYLPGGYPELHMDKLA
EEEEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHH
LNHALKQQIIKHIEQGKPCLAECGGMLYLNKALTDTESQTENMVGALNAKATMQKKLAAL
HHHHHHHHHHHHHHCCCCHHHHCCCEEEEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHH
GILEANFDGQMLRGHTFHYSKTDSKETVLTQPESQYGRPTDPVWIKQSTIASYAHWYFPY
HEEEECCCCCEECCCEEECCCCCCCCEEEECCHHHCCCCCCCEEEEHHHHHHHHHEECCC
NPDLVAKIFKGEFY
CHHHHHHHHCCCCC
>Mature Secondary Structure 
STAYCSALVVGAPSSGSGKTTVVAALARALVQQGKKVRVFKTGPDFIDPQFLSIASDTP
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEECCCCC
TYQLDLWMCGEAECQHLIYQAALEADVILIEGVMGMFDGKCSSADIAAKFNLPMLAVVDA
CEEEEEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCEEEECCCCEEEEECC
GAMAQTFGAIVNGLSTFRDDVDVFGVVANRVGSERHAEMLKESLKPETEFCGWLPKDMDL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCC
SLPERHLGLVQAQELDDIEERLNHAAKLLLKHGEIPLPTAQAFEKPKQALPARHESLTGH
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCCHHHCCCCC
TIAVARDNSFAFLYQANLDLLIELGAKLSFFSPLAHDDLPVCDALYLPGGYPELHMDKLA
EEEEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHH
LNHALKQQIIKHIEQGKPCLAECGGMLYLNKALTDTESQTENMVGALNAKATMQKKLAAL
HHHHHHHHHHHHHHCCCCHHHHCCCEEEEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHH
GILEANFDGQMLRGHTFHYSKTDSKETVLTQPESQYGRPTDPVWIKQSTIASYAHWYFPY
HEEEECCCCCEECCCEEECCCCCCCCEEEECCHHHCCCCCCCEEEEHHHHHHHHHEECCC
NPDLVAKIFKGEFY
CHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA