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The map label for this gene is gloB

Identifier: 218710295

GI number: 218710295

Start: 2496445

End: 2497203

Strand: Direct

Name: gloB

Synonym: VS_2329

Alternate gene names: 218710295

Gene position: 2496445-2497203 (Clockwise)

Preceding gene: 218710291

Following gene: 218710296

Centisome position: 75.67

GC content: 46.25

Gene sequence:

>759_bases
ATGTTACATATCAAAAGCATACCTGCATTTAACGACAATTACATCTGGCTGATTCAAAATAGCGATCGCCGTTGTGCTGT
TGTCGACCCTGGTGATGCAGCTCCAGTATTAGAGTACTTAGCACACCATGAGCTAACTTTAGATGCAATCTTGATTACGC
ACCACCACCACGATCACATTGGTGGCGTGCCAGAGTTGGTAAGACAATTCCCTGGCGTGGATGTCGTTGGCCCCAAAAAT
GAACCTATCCCGACCTTAACGCATCCCGTTGATGACGGTGACCAATTAGAGCTGTTTGGCGAAGTATTCCTCGTGCTAGG
GCTTAGTGGGCACACCGCTGGGCACATTGGTTATGTTGGTGATGCCAAACTGTTTTGTGGCGATGTACTGTTTTCAGCTG
GTTGTGGGCGAATCATGGAAGGCACACCACAGCAGATGTTTGATGCATTGAACAAGATCACAGCACTCCCACAAGAAACC
GAGGTTTACTGTGCTCATGAATACACGGCAGCCAATATCGCTTTTGCACTGGCTGTTGAGCCTGACAACCAACATTTGCA
ACAATACCGCGACCAAGTGAATCGGCTTCGAGCCCAAAATAAGTCGACCATCCCCACAAATTTAAGACAAGAAAAGTTTA
TTAACCCCTTCTTACGCTACACAGAACCAAGTGTAGTGAAATCAGTATCTAATCGTACTGAGCAGACCGATCCTTTATCG
GTTTTTACCGCTTTACGTGCGTGGAAGAACGAATTTTAA

Upstream 100 bases:

>100_bases
CAAGTATAAGGACGCTCAAACTTCTTTCTGCTACGTGCTGGCTTCATCAATAATCTGTCTCACTTATTCTGTCTCACTAA
AAGTGACTGGAGAACCAATA

Downstream 100 bases:

>100_bases
CAAATACAGGCTTGTCACTCATAGGGAGTGGCAAGTATTATCATCGGCCGTTTATTAAAAAGGCTGTAACATGCGAGTTA
AGTACAGCTGGGCTTTGGTA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLHIKSIPAFNDNYIWLIQNSDRRCAVVDPGDAAPVLEYLAHHELTLDAILITHHHHDHIGGVPELVRQFPGVDVVGPKN
EPIPTLTHPVDDGDQLELFGEVFLVLGLSGHTAGHIGYVGDAKLFCGDVLFSAGCGRIMEGTPQQMFDALNKITALPQET
EVYCAHEYTAANIAFALAVEPDNQHLQQYRDQVNRLRAQNKSTIPTNLRQEKFINPFLRYTEPSVVKSVSNRTEQTDPLS
VFTALRAWKNEF

Sequences:

>Translated_252_residues
MLHIKSIPAFNDNYIWLIQNSDRRCAVVDPGDAAPVLEYLAHHELTLDAILITHHHHDHIGGVPELVRQFPGVDVVGPKN
EPIPTLTHPVDDGDQLELFGEVFLVLGLSGHTAGHIGYVGDAKLFCGDVLFSAGCGRIMEGTPQQMFDALNKITALPQET
EVYCAHEYTAANIAFALAVEPDNQHLQQYRDQVNRLRAQNKSTIPTNLRQEKFINPFLRYTEPSVVKSVSNRTEQTDPLS
VFTALRAWKNEF
>Mature_252_residues
MLHIKSIPAFNDNYIWLIQNSDRRCAVVDPGDAAPVLEYLAHHELTLDAILITHHHHDHIGGVPELVRQFPGVDVVGPKN
EPIPTLTHPVDDGDQLELFGEVFLVLGLSGHTAGHIGYVGDAKLFCGDVLFSAGCGRIMEGTPQQMFDALNKITALPQET
EVYCAHEYTAANIAFALAVEPDNQHLQQYRDQVNRLRAQNKSTIPTNLRQEKFINPFLRYTEPSVVKSVSNRTEQTDPLS
VFTALRAWKNEF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI14150041, Length=260, Percent_Identity=37.3076923076923, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=35, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=35, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI116642887, Length=221, Percent_Identity=38.0090497737557, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI21703352, Length=221, Percent_Identity=38.0090497737557, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=40.5128205128205, Blast_Score=137, Evalue=7e-33,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=45.0199203187251, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1787158, Length=160, Percent_Identity=35, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=265, Percent_Identity=36.2264150943396, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538952, Length=194, Percent_Identity=28.3505154639175, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=28.7878787878788, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=259, Percent_Identity=31.2741312741313, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=39.0804597701149, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=39.0804597701149, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=39.0804597701149, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_VIBSL (B7VIP5)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417916.1
- GeneID:   7161870
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2329
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK874727
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28143; Mature: 28143

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHIKSIPAFNDNYIWLIQNSDRRCAVVDPGDAAPVLEYLAHHELTLDAILITHHHHDHI
CEEECCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCC
GGVPELVRQFPGVDVVGPKNEPIPTLTHPVDDGDQLELFGEVFLVLGLSGHTAGHIGYVG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECC
DAKLFCGDVLFSAGCGRIMEGTPQQMFDALNKITALPQETEVYCAHEYTAANIAFALAVE
CHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECEEECEEEEEEEEC
PDNQHLQQYRDQVNRLRAQNKSTIPTNLRQEKFINPFLRYTEPSVVKSVSNRTEQTDPLS
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
VFTALRAWKNEF
HHHHHHHHHCCC
>Mature Secondary Structure
MLHIKSIPAFNDNYIWLIQNSDRRCAVVDPGDAAPVLEYLAHHELTLDAILITHHHHDHI
CEEECCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCC
GGVPELVRQFPGVDVVGPKNEPIPTLTHPVDDGDQLELFGEVFLVLGLSGHTAGHIGYVG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECC
DAKLFCGDVLFSAGCGRIMEGTPQQMFDALNKITALPQETEVYCAHEYTAANIAFALAVE
CHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECEEECEEEEEEEEC
PDNQHLQQYRDQVNRLRAQNKSTIPTNLRQEKFINPFLRYTEPSVVKSVSNRTEQTDPLS
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
VFTALRAWKNEF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA