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The map label for this gene is rnhA

Identifier: 218710291

GI number: 218710291

Start: 2493790

End: 2494254

Strand: Direct

Name: rnhA

Synonym: VS_2325

Alternate gene names: 218710291

Gene position: 2493790-2494254 (Clockwise)

Preceding gene: 218710288

Following gene: 218710295

Centisome position: 75.59

GC content: 50.32

Gene sequence:

>465_bases
ATGACGAAACAAGTTGAAATTTTCACTGATGGTTCTTGTTTAGGCAACCCAGGTCCTGGCGGCTACGGCATCGTACTTCG
CTACAGAAAAGTCGAGAAGACGTTAGCCGAAGGTTTCACCTTAACGACCAACAACCGTATGGAAATGCTCGCCGCTGTTG
TGGCTCTTCAAGCCCTCAAAGAGCCTTGCTCTGTCATTCTGACCACAGATAGCCAATACGTGCGTCAAGGTATCACCCAG
TGGATTCACAACTGGAAAAAGCGTGACTGGAAAACCGCTGACAAGAAACCGGTTAAAAACGCAGACCTGTGGCAAAGGCT
CGATAAAGAGACGGCGCGCCATAGCGTTGATTGGCGCTGGGTTAAGGGGCACGCGGGGCACCGAGAAAATGAAATGTGTG
ATGATTTAGCTCGAAGTGCAGCTGAAAACCCGACCCAAGAAGATACGGGCTATCAGCCAAGCTGA

Upstream 100 bases:

>100_bases
TTTTTTTCGTTCGTGTTTTGTTCTGGAGTGCTACTGGTATTCATAAGGATAACTGTGTCAGACTATGCCGATAATGTGCT
TGAGGTAATAGTATCAAATC

Downstream 100 bases:

>100_bases
TCATTTTCTTAAAGGAAATGTCAGTATCTAAAACAGATATTGACCCGTCCTGATATGGGTTGACACTTGATTGACTAAAA
CGCTCGATGTACTTCATCGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTKQVEIFTDGSCLGNPGPGGYGIVLRYRKVEKTLAEGFTLTTNNRMEMLAAVVALQALKEPCSVILTTDSQYVRQGITQ
WIHNWKKRDWKTADKKPVKNADLWQRLDKETARHSVDWRWVKGHAGHRENEMCDDLARSAAENPTQEDTGYQPS

Sequences:

>Translated_154_residues
MTKQVEIFTDGSCLGNPGPGGYGIVLRYRKVEKTLAEGFTLTTNNRMEMLAAVVALQALKEPCSVILTTDSQYVRQGITQ
WIHNWKKRDWKTADKKPVKNADLWQRLDKETARHSVDWRWVKGHAGHRENEMCDDLARSAAENPTQEDTGYQPS
>Mature_153_residues
TKQVEIFTDGSCLGNPGPGGYGIVLRYRKVEKTLAEGFTLTTNNRMEMLAAVVALQALKEPCSVILTTDSQYVRQGITQW
IHNWKKRDWKTADKKPVKNADLWQRLDKETARHSVDWRWVKGHAGHRENEMCDDLARSAAENPTQEDTGYQPS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=34.0136054421769, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=77.6315789473684, Blast_Score=257, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17505482, Length=133, Percent_Identity=34.5864661654135, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=36.0544217687075, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=36.0544217687075, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_VIBSL (B7VIP1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417912.1
- ProteinModelPortal:   B7VIP1
- GeneID:   7161866
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2325
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17533; Mature: 17402

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVEIFTDGSCLGNPGPGGYGIVLRYRKVEKTLAEGFTLTTNNRMEMLAAVVALQALK
CCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
EPCSVILTTDSQYVRQGITQWIHNWKKRDWKTADKKPVKNADLWQRLDKETARHSVDWRW
HCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VKGHAGHRENEMCDDLARSAAENPTQEDTGYQPS
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKQVEIFTDGSCLGNPGPGGYGIVLRYRKVEKTLAEGFTLTTNNRMEMLAAVVALQALK
CCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHH
EPCSVILTTDSQYVRQGITQWIHNWKKRDWKTADKKPVKNADLWQRLDKETARHSVDWRW
HCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VKGHAGHRENEMCDDLARSAAENPTQEDTGYQPS
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA