Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 218710296

GI number: 218710296

Start: 2497274

End: 2498845

Strand: Direct

Name: mltD [H]

Synonym: VS_2330

Alternate gene names: 218710296

Gene position: 2497274-2498845 (Clockwise)

Preceding gene: 218710295

Following gene: 218710297

Centisome position: 75.69

GC content: 45.74

Gene sequence:

>1572_bases
ATGCGAGTTAAGTACAGCTGGGCTTTGGTACTACTACTTTCTGGTTGCCAATTAACTCAGTCAGAGAATCCAGACCAAGC
TTCCGAGCAAACCAACACCTCCCCTACTAAAGAAGTTTCTCAAGCGAACGTTTCATCGGGAGCGATCCAAGAACAACCAA
AAGTTGAAACACCCGTCGTTACTCCACAAACACAGCAAGATGTTTGGAAACGTATTGCGATGCAACTTGAAATGGAAGTG
CCGGACCAAAAGAAAGTCGACTACTACCGCACTTGGTACCTCAAGCACCCTGGTCATCTAAAAACTGTATCAAAACGCGC
TGAACCTTTCCTTTATTTGATCACAACTAAGATTGAAGAAAGAGACTTGCCTTTGGAGCTGGCCCTGTTGCCCGTCGTTG
AAAGCTCGTTTGATGCATTTGCTTATTCTCACGGTAGCGCTGCTGGACTATGGCAGTTCATTTCGGGTACTGGCAAAGCT
TACGGACTAGAACAAAACTTCTGGTACGATGGCCGCCGTGACGTAGCCGCTTCAACCGACGCTGCATTGGATTTCTTAAC
CGACCTGAACAAACGTTTTGATGGGAACTGGCAACACGCAATCGCTGCCTACAACAGTGGCGGTGGTCGTGTGAACAGCG
CAATCCGTAAGAACAAGAAGCTGGGTAAGCCAATCGACTTCTTCTCTCTAGACTTACCAAAAGAGACCAGCAGCTATGTA
CCTAAGCTACTTGCACTGGCCGACGTGATTGCCAACCAAGAAAAGTATGGCATCGATATCCCTGCGATCCCGAACAAGGC
AGTACTGACTCTGGTTAACCCAAATGAACAACTTGATTTAGCCATTGCCGCAAACTACGCAGGCATTCCGGTAAAAGAGC
TACAAGGCTACAACCCAGCTTATAACCAATGGGCGACGGCACCAGAAAAACATCAGCAATTATTGCTTCCTCTAAGCTCT
GTTGAGAAGTTCAACAAAGAAGTGGCAGCCAATAAAGGCAAAGGCATGAAGTTGGTGCGCTATAAAGTGCAATCTGGCGA
TAGCATCAGCGTACTGGCGAGTAAATACAACACCACCAGCAAAGTGATCCGCTCTGCAAACGGTTTGAGCAACAATAATA
TTCGTGTTGGTCAGCACCTATTTATTCCAACATCAACCAAAGACGATAAGACATACGCACTAAGCGCTTCAAATCGCCTA
GCAAGCACACAGTCAAAGAGTCGTGGTCAATATAAGATTAGCCATACCGTAAAAAGTGGTGATAGCCTATGGACAATTGC
GCGCGCAAATAAGGTCTCTCACCAATCACTGGCTAAGTGGAATGGCATGGGACCACGTGACACACTTAGAATTGGTCAAG
AACTGGTCATTTGGAAGAATGGTTCAGAGGGTGCCATCATCCGAACTGTCTTTTATAACGTAAGATCAGGTGACACAGTC
AGCGGTATTGCATCAAAGTTCAAAGTAAAAAGCGCAGATGTTGTAAAATGGAACGCTTTGCAGAACAAGAAATACTTACA
GCCAGGACAAAAACTGAAGCTGTATGTTGATGTAACTAAGGTAAGTGTATGA

Upstream 100 bases:

>100_bases
GCTTTACGTGCGTGGAAGAACGAATTTTAACAAATACAGGCTTGTCACTCATAGGGAGTGGCAAGTATTATCATCGGCCG
TTTATTAAAAAGGCTGTAAC

Downstream 100 bases:

>100_bases
ACCCGTCAAGTAACCCACTCGTCATGCTGTTGGATATATTCCGTTCACCAACAGCTTGTTTCTTAGCGCTTTATCAAAGA
AGTGCTTGGGGATGGCAACC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MRVKYSWALVLLLSGCQLTQSENPDQASEQTNTSPTKEVSQANVSSGAIQEQPKVETPVVTPQTQQDVWKRIAMQLEMEV
PDQKKVDYYRTWYLKHPGHLKTVSKRAEPFLYLITTKIEERDLPLELALLPVVESSFDAFAYSHGSAAGLWQFISGTGKA
YGLEQNFWYDGRRDVAASTDAALDFLTDLNKRFDGNWQHAIAAYNSGGGRVNSAIRKNKKLGKPIDFFSLDLPKETSSYV
PKLLALADVIANQEKYGIDIPAIPNKAVLTLVNPNEQLDLAIAANYAGIPVKELQGYNPAYNQWATAPEKHQQLLLPLSS
VEKFNKEVAANKGKGMKLVRYKVQSGDSISVLASKYNTTSKVIRSANGLSNNNIRVGQHLFIPTSTKDDKTYALSASNRL
ASTQSKSRGQYKISHTVKSGDSLWTIARANKVSHQSLAKWNGMGPRDTLRIGQELVIWKNGSEGAIIRTVFYNVRSGDTV
SGIASKFKVKSADVVKWNALQNKKYLQPGQKLKLYVDVTKVSV

Sequences:

>Translated_523_residues
MRVKYSWALVLLLSGCQLTQSENPDQASEQTNTSPTKEVSQANVSSGAIQEQPKVETPVVTPQTQQDVWKRIAMQLEMEV
PDQKKVDYYRTWYLKHPGHLKTVSKRAEPFLYLITTKIEERDLPLELALLPVVESSFDAFAYSHGSAAGLWQFISGTGKA
YGLEQNFWYDGRRDVAASTDAALDFLTDLNKRFDGNWQHAIAAYNSGGGRVNSAIRKNKKLGKPIDFFSLDLPKETSSYV
PKLLALADVIANQEKYGIDIPAIPNKAVLTLVNPNEQLDLAIAANYAGIPVKELQGYNPAYNQWATAPEKHQQLLLPLSS
VEKFNKEVAANKGKGMKLVRYKVQSGDSISVLASKYNTTSKVIRSANGLSNNNIRVGQHLFIPTSTKDDKTYALSASNRL
ASTQSKSRGQYKISHTVKSGDSLWTIARANKVSHQSLAKWNGMGPRDTLRIGQELVIWKNGSEGAIIRTVFYNVRSGDTV
SGIASKFKVKSADVVKWNALQNKKYLQPGQKLKLYVDVTKVSV
>Mature_523_residues
MRVKYSWALVLLLSGCQLTQSENPDQASEQTNTSPTKEVSQANVSSGAIQEQPKVETPVVTPQTQQDVWKRIAMQLEMEV
PDQKKVDYYRTWYLKHPGHLKTVSKRAEPFLYLITTKIEERDLPLELALLPVVESSFDAFAYSHGSAAGLWQFISGTGKA
YGLEQNFWYDGRRDVAASTDAALDFLTDLNKRFDGNWQHAIAAYNSGGGRVNSAIRKNKKLGKPIDFFSLDLPKETSSYV
PKLLALADVIANQEKYGIDIPAIPNKAVLTLVNPNEQLDLAIAANYAGIPVKELQGYNPAYNQWATAPEKHQQLLLPLSS
VEKFNKEVAANKGKGMKLVRYKVQSGDSISVLASKYNTTSKVIRSANGLSNNNIRVGQHLFIPTSTKDDKTYALSASNRL
ASTQSKSRGQYKISHTVKSGDSLWTIARANKVSHQSLAKWNGMGPRDTLRIGQELVIWKNGSEGAIIRTVFYNVRSGDTV
SGIASKFKVKSADVVKWNALQNKKYLQPGQKLKLYVDVTKVSV

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=454, Percent_Identity=35.4625550660793, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58001; Mature: 58001

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKYSWALVLLLSGCQLTQSENPDQASEQTNTSPTKEVSQANVSSGAIQEQPKVETPVV
CEEEHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
TPQTQQDVWKRIAMQLEMEVPDQKKVDYYRTWYLKHPGHLKTVSKRAEPFLYLITTKIEE
CCCCHHHHHHHHHHEEEECCCCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEEEECCC
RDLPLELALLPVVESSFDAFAYSHGSAAGLWQFISGTGKAYGLEQNFWYDGRRDVAASTD
CCCCEEEEEEHHHHCCCHHHEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHH
AALDFLTDLNKRFDGNWQHAIAAYNSGGGRVNSAIRKNKKLGKPIDFFSLDLPKETSSYV
HHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHH
PKLLALADVIANQEKYGIDIPAIPNKAVLTLVNPNEQLDLAIAANYAGIPVKELQGYNPA
HHHHHHHHHHHCCHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHCCCCCC
YNQWATAPEKHQQLLLPLSSVEKFNKEVAANKGKGMKLVRYKVQSGDSISVLASKYNTTS
HHHCCCCCHHHHHEECCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCHHH
KVIRSANGLSNNNIRVGQHLFIPTSTKDDKTYALSASNRLASTQSKSRGQYKISHTVKSG
HHHHHCCCCCCCCEEECCEEEEECCCCCCCEEEEECCCCHHHCCCCCCCCEEEEEEECCC
DSLWTIARANKVSHQSLAKWNGMGPRDTLRIGQELVIWKNGSEGAIIRTVFYNVRSGDTV
CCEEEEECCCCCCHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCH
SGIASKFKVKSADVVKWNALQNKKYLQPGQKLKLYVDVTKVSV
HHHHHHHEECCCCEEEEECCCCCHHCCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MRVKYSWALVLLLSGCQLTQSENPDQASEQTNTSPTKEVSQANVSSGAIQEQPKVETPVV
CEEEHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
TPQTQQDVWKRIAMQLEMEVPDQKKVDYYRTWYLKHPGHLKTVSKRAEPFLYLITTKIEE
CCCCHHHHHHHHHHEEEECCCCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEEEECCC
RDLPLELALLPVVESSFDAFAYSHGSAAGLWQFISGTGKAYGLEQNFWYDGRRDVAASTD
CCCCEEEEEEHHHHCCCHHHEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHH
AALDFLTDLNKRFDGNWQHAIAAYNSGGGRVNSAIRKNKKLGKPIDFFSLDLPKETSSYV
HHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHH
PKLLALADVIANQEKYGIDIPAIPNKAVLTLVNPNEQLDLAIAANYAGIPVKELQGYNPA
HHHHHHHHHHHCCHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHCCCCCC
YNQWATAPEKHQQLLLPLSSVEKFNKEVAANKGKGMKLVRYKVQSGDSISVLASKYNTTS
HHHCCCCCHHHHHEECCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEEECCCHHH
KVIRSANGLSNNNIRVGQHLFIPTSTKDDKTYALSASNRLASTQSKSRGQYKISHTVKSG
HHHHHCCCCCCCCEEECCEEEEECCCCCCCEEEEECCCCHHHCCCCCCCCEEEEEEECCC
DSLWTIARANKVSHQSLAKWNGMGPRDTLRIGQELVIWKNGSEGAIIRTVFYNVRSGDTV
CCEEEEECCCCCCHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCH
SGIASKFKVKSADVVKWNALQNKKYLQPGQKLKLYVDVTKVSV
HHHHHHHEECCCCEEEEECCCCCHHCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]