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The map label for this gene is yfcE [H]

Identifier: 218710240

GI number: 218710240

Start: 2444470

End: 2445015

Strand: Reverse

Name: yfcE [H]

Synonym: VS_2273

Alternate gene names: 218710240

Gene position: 2445015-2444470 (Counterclockwise)

Preceding gene: 218710241

Following gene: 218710236

Centisome position: 74.11

GC content: 42.67

Gene sequence:

>546_bases
GTGAAATTATTTTTTGCTTCAGACCTACACGGTTCGCTACCAGCAACAGAAAAGGTATTAGAACTCTACCAAGCGTCTGG
TGCGCAATATTTAGTGCTATTGGGTGACATTCTGAATCATGGGCCAAGAAATCCGATTCCAGAAGGATACAATCCACCGG
CGGTTGCAGAGAAGTTGAATGCGTTTTCTCAAGAGATCATCGCCGTTCGTGGTAACTGCGACAGTGAAGTGGATCAAATG
CTGTTGTCTTTTCCAATGATGATGGATTACTCATGGGTTTTATTGGAATCAGGTCAGCGTATCTTTCTAACCCATGGTCA
TTTGTATAACACCACTAAACGACCAGCACTAAAAGCGGGTGATATTATTGCCCATGGTCATACTCATATTCCTGTTGCTG
AATACCAAGATGAGATCTTTATCTTTAATCCTAGCTCGGTGACATTCCCACGAGATGGTCACGCGGCTAGCTACGGTGTA
TATGAAAATAAAACGTTCAAAGTTATTAGCCTTGAGGGTGACGTAATTGTGAGTGGTGAGCTTTAA

Upstream 100 bases:

>100_bases
TGGTTAGTTACAATAATATCGTGATCAACGGTATGGCGAGAGATGACGTGTAGCATTTAGATACGTACAATAGGCTCATA
AATTTTATGGAGAAGTACGT

Downstream 100 bases:

>100_bases
ATAAACTCAATCGGCGGCGTTAGAAAAGGCTCACTTTGAGTCTACGTGGCCGAGGTCCCTGTCTGGAGCAATGATGTTTC
GCACTCTTTGCTTCAGAACT

Product: phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKLFFASDLHGSLPATEKVLELYQASGAQYLVLLGDILNHGPRNPIPEGYNPPAVAEKLNAFSQEIIAVRGNCDSEVDQM
LLSFPMMMDYSWVLLESGQRIFLTHGHLYNTTKRPALKAGDIIAHGHTHIPVAEYQDEIFIFNPSSVTFPRDGHAASYGV
YENKTFKVISLEGDVIVSGEL

Sequences:

>Translated_181_residues
MKLFFASDLHGSLPATEKVLELYQASGAQYLVLLGDILNHGPRNPIPEGYNPPAVAEKLNAFSQEIIAVRGNCDSEVDQM
LLSFPMMMDYSWVLLESGQRIFLTHGHLYNTTKRPALKAGDIIAHGHTHIPVAEYQDEIFIFNPSSVTFPRDGHAASYGV
YENKTFKVISLEGDVIVSGEL
>Mature_181_residues
MKLFFASDLHGSLPATEKVLELYQASGAQYLVLLGDILNHGPRNPIPEGYNPPAVAEKLNAFSQEIIAVRGNCDSEVDQM
LLSFPMMMDYSWVLLESGQRIFLTHGHLYNTTKRPALKAGDIIAHGHTHIPVAEYQDEIFIFNPSSVTFPRDGHAASYGV
YENKTFKVISLEGDVIVSGEL

Specific function: Shows phosphodiesterase activity [H]

COG id: COG0622

COG function: function code R; Predicted phosphoesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily. YfcE family [H]

Homologues:

Organism=Escherichia coli, GI1788638, Length=178, Percent_Identity=60.1123595505618, Blast_Score=228, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR020935
- InterPro:   IPR000979 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 20035; Mature: 20035

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFFASDLHGSLPATEKVLELYQASGAQYLVLLGDILNHGPRNPIPEGYNPPAVAEKLN
CEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHHH
AFSQEIIAVRGNCDSEVDQMLLSFPMMMDYSWVLLESGQRIFLTHGHLYNTTKRPALKAG
HHHHEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCEECCCCCCCCCCC
DIIAHGHTHIPVAEYQDEIFIFNPSSVTFPRDGHAASYGVYENKTFKVISLEGDVIVSGE
CEEECCCCCCCHHHCCCEEEEECCCCEECCCCCCCEECCEECCCEEEEEEECCCEEEECC
L
C
>Mature Secondary Structure
MKLFFASDLHGSLPATEKVLELYQASGAQYLVLLGDILNHGPRNPIPEGYNPPAVAEKLN
CEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHHH
AFSQEIIAVRGNCDSEVDQMLLSFPMMMDYSWVLLESGQRIFLTHGHLYNTTKRPALKAG
HHHHEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCEECCCCCCCCCCC
DIIAHGHTHIPVAEYQDEIFIFNPSSVTFPRDGHAASYGVYENKTFKVISLEGDVIVSGE
CEEECCCCCCCHHHCCCEEEEECCCCEECCCCCCCEECCEECCCEEEEEEECCCEEEECC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]