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The map label for this gene is dctD [H]

Identifier: 218710177

GI number: 218710177

Start: 2359399

End: 2360748

Strand: Direct

Name: dctD [H]

Synonym: VS_2208

Alternate gene names: 218710177

Gene position: 2359399-2360748 (Clockwise)

Preceding gene: 218710176

Following gene: 218710181

Centisome position: 71.51

GC content: 46.3

Gene sequence:

>1350_bases
ATGTGTCACGTCTATTTCATTGATGATGAATCCGATCTGAGAATGGCTATTGAACAGAGCTTTGAGCTCGCCGATATTGA
TGCCGAATTCTTTCCCGATGCGGAGTCTGCTCTGCTCGCGATTCAAGAAAACGGCTTACCTCACGTGATCATCACTGATA
TCTGTCTACCAGGTCTATCCGGGCATGATCTGCTCAACACCGTGATGCACAAAGACAAAGAGATCCCCGTGATTATGATT
ACTGGTCACGGCGATATTTCGATGGCTGTACAAGCGATCCAAGATGGCGCCTATGACTTCATCGAAAAGCCGTTCGCCAA
TGAACGTCTAATCGAAACCACCAAACGAGCAATAGAAAAACGTCAACTCACACTCGAGAATCTTGAATTGAAGCGCTCTT
TAAAGGCCAGTAAAGCGCTTGGGCCGAGAATCATTGGTGACACGCAATCAATGACCGACCTGCGTTCTATCATCACTCAT
GTGGCAGATACTAACGCTGACATTTTACTCTTTGGTGAAACTGGCACAGGCAAAGAGTTGGTCGCACGCTCTCTACATGA
ACAAAGCAGCCGGCGAGAACAAAACTTCGTCGCCGTCAATTGCGGTGCGGTACCTGAAAACCTGATAGAGAGTGAACTGT
ATGGTCATGAGAAAGGTGCGTTCACTGGCGCAGAAAGCAAGCGAGTGGGTAAATTTGAATTTGCTCAAGGCGGAACTCTA
TTCTTAGATGAAATTGAGTCCATGCCGATGCAGGCACAAATTCGCCTGTTGCGCGTCTTACAAGAGCGCGTTATTGAAAG
GGTTGGCTCAAACGGTTTAGTGCCTCTCGATATTCGAGTAATCGCCGCAACCAAAGTCGACCTAAAGAAGGCGGCTGAAG
AAGGCGTTTTCCGCCAAGACCTCTATTATCGACTCAACGTTGTCACCTTGGATTTACCGCCGTTAAGAAGCCGTCAAGAA
GATATCCCAGCACTCTTCCACCACTTCTTACTGGTCGCTGCTGCACGTTATGGCAAGACAGCACCAGCACTTCCACAAAA
GGAGCTGCACGCGCTTTTAGCCCATGATTGGCCGGGAAATGTACGAGAATTGCGTAATACAGCTGAGCGGTTTGTACTTT
TAGGTAAGCTTTCTCACTTATCAGACAACACCAATCAAGTCACTCAAGAACTGTCTTTGGCAGAACTGGTGTCTGACTTT
GAGAAAAACACGTTAAAACAAGCACTTATTGAATGTAATGGCAGTATTAAACAGACTATGGATAGACTGCAACTTCCACG
TAAAACCCTCTACGATAAAATGCAAAAACACCAACTAGTTAAAGAGTCATACCGACAGAGCGAAAACTAA

Upstream 100 bases:

>100_bases
TATCGAGTTACCCATAGTTCCAACAAAAGAATCAGATTAGTCGCTAAACAAACCAGTACACCTTCACCAACAATAAAAAT
AAAAGATTAAAGGAATAACT

Downstream 100 bases:

>100_bases
ATTAAATTTGGGAGCAAGATCACCTATACTTATTTCACAACAGGATGTTATGCAACCAAGGTGGTAAGGAGGTCATTATG
GGTGAGAAAACAAAAGTTCC

Product: C4-dicarboxylate transport transcriptional regulatory protein DctD

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MCHVYFIDDESDLRMAIEQSFELADIDAEFFPDAESALLAIQENGLPHVIITDICLPGLSGHDLLNTVMHKDKEIPVIMI
TGHGDISMAVQAIQDGAYDFIEKPFANERLIETTKRAIEKRQLTLENLELKRSLKASKALGPRIIGDTQSMTDLRSIITH
VADTNADILLFGETGTGKELVARSLHEQSSRREQNFVAVNCGAVPENLIESELYGHEKGAFTGAESKRVGKFEFAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERVGSNGLVPLDIRVIAATKVDLKKAAEEGVFRQDLYYRLNVVTLDLPPLRSRQE
DIPALFHHFLLVAAARYGKTAPALPQKELHALLAHDWPGNVRELRNTAERFVLLGKLSHLSDNTNQVTQELSLAELVSDF
EKNTLKQALIECNGSIKQTMDRLQLPRKTLYDKMQKHQLVKESYRQSEN

Sequences:

>Translated_449_residues
MCHVYFIDDESDLRMAIEQSFELADIDAEFFPDAESALLAIQENGLPHVIITDICLPGLSGHDLLNTVMHKDKEIPVIMI
TGHGDISMAVQAIQDGAYDFIEKPFANERLIETTKRAIEKRQLTLENLELKRSLKASKALGPRIIGDTQSMTDLRSIITH
VADTNADILLFGETGTGKELVARSLHEQSSRREQNFVAVNCGAVPENLIESELYGHEKGAFTGAESKRVGKFEFAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERVGSNGLVPLDIRVIAATKVDLKKAAEEGVFRQDLYYRLNVVTLDLPPLRSRQE
DIPALFHHFLLVAAARYGKTAPALPQKELHALLAHDWPGNVRELRNTAERFVLLGKLSHLSDNTNQVTQELSLAELVSDF
EKNTLKQALIECNGSIKQTMDRLQLPRKTLYDKMQKHQLVKESYRQSEN
>Mature_449_residues
MCHVYFIDDESDLRMAIEQSFELADIDAEFFPDAESALLAIQENGLPHVIITDICLPGLSGHDLLNTVMHKDKEIPVIMI
TGHGDISMAVQAIQDGAYDFIEKPFANERLIETTKRAIEKRQLTLENLELKRSLKASKALGPRIIGDTQSMTDLRSIITH
VADTNADILLFGETGTGKELVARSLHEQSSRREQNFVAVNCGAVPENLIESELYGHEKGAFTGAESKRVGKFEFAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERVGSNGLVPLDIRVIAATKVDLKKAAEEGVFRQDLYYRLNVVTLDLPPLRSRQE
DIPALFHHFLLVAAARYGKTAPALPQKELHALLAHDWPGNVRELRNTAERFVLLGKLSHLSDNTNQVTQELSLAELVSDF
EKNTLKQALIECNGSIKQTMDRLQLPRKTLYDKMQKHQLVKESYRQSEN

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=421, Percent_Identity=40.6175771971496, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788550, Length=450, Percent_Identity=36.8888888888889, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1790437, Length=402, Percent_Identity=38.3084577114428, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=43.8438438438438, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788905, Length=406, Percent_Identity=37.4384236453202, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1789087, Length=400, Percent_Identity=38.5, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=39.2523364485981, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=41.6666666666667, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=254, Percent_Identity=43.7007874015748, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=36.3636363636364, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1787583, Length=292, Percent_Identity=36.3013698630137, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=290, Percent_Identity=32.4137931034483, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=38.3259911894273, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50442; Mature: 50442

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCHVYFIDDESDLRMAIEQSFELADIDAEFFPDAESALLAIQENGLPHVIITDICLPGLS
CEEEEEECCCHHHHHHHHHCCCEECCCHHHCCCHHHHEEEEECCCCCEEEEHHHHCCCCC
GHDLLNTVMHKDKEIPVIMITGHGDISMAVQAIQDGAYDFIEKPFANERLIETTKRAIEK
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHH
RQLTLENLELKRSLKASKALGPRIIGDTQSMTDLRSIITHVADTNADILLFGETGTGKEL
HHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
VARSLHEQSSRREQNFVAVNCGAVPENLIESELYGHEKGAFTGAESKRVGKFEFAQGGTL
HHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEIESMPMQAQIRLLRVLQERVIERVGSNGLVPLDIRVIAATKVDLKKAAEEGVFRQD
EHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHCCHHHHH
LYYRLNVVTLDLPPLRSRQEDIPALFHHFLLVAAARYGKTAPALPQKELHALLAHDWPGN
HEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
VRELRNTAERFVLLGKLSHLSDNTNQVTQELSLAELVSDFEKNTLKQALIECNGSIKQTM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DRLQLPRKTLYDKMQKHQLVKESYRQSEN
HHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCHVYFIDDESDLRMAIEQSFELADIDAEFFPDAESALLAIQENGLPHVIITDICLPGLS
CEEEEEECCCHHHHHHHHHCCCEECCCHHHCCCHHHHEEEEECCCCCEEEEHHHHCCCCC
GHDLLNTVMHKDKEIPVIMITGHGDISMAVQAIQDGAYDFIEKPFANERLIETTKRAIEK
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHH
RQLTLENLELKRSLKASKALGPRIIGDTQSMTDLRSIITHVADTNADILLFGETGTGKEL
HHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
VARSLHEQSSRREQNFVAVNCGAVPENLIESELYGHEKGAFTGAESKRVGKFEFAQGGTL
HHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEE
FLDEIESMPMQAQIRLLRVLQERVIERVGSNGLVPLDIRVIAATKVDLKKAAEEGVFRQD
EHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHCCHHHHH
LYYRLNVVTLDLPPLRSRQEDIPALFHHFLLVAAARYGKTAPALPQKELHALLAHDWPGN
HEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
VRELRNTAERFVLLGKLSHLSDNTNQVTQELSLAELVSDFEKNTLKQALIECNGSIKQTM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DRLQLPRKTLYDKMQKHQLVKESYRQSEN
HHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]