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The map label for this gene is dctB [H]

Identifier: 218710176

GI number: 218710176

Start: 2357530

End: 2359338

Strand: Direct

Name: dctB [H]

Synonym: VS_2207

Alternate gene names: 218710176

Gene position: 2357530-2359338 (Clockwise)

Preceding gene: 218710168

Following gene: 218710177

Centisome position: 71.46

GC content: 44.83

Gene sequence:

>1809_bases
ATGTTTCAAGCTTTGAGAATCCCAATCCTATTAATGGCCACATACAGTTTACTAATGGTGTTCGGCGGTCATTGGGTGTG
GAACACAAGCCATGAAAGTTTGTTAAATGATCATCAATCTAAGCTAGACCGATTTTCGGTTCATATTTCAAGCCAACTCG
ACAAGTTCGCGCACATTCCCGAGCTGCTTTCAAAAGACAAAGAGTTGGTCGATGCTCTTCACTCTCCAAGTAACTCTGCG
CAAATCGAACTCACCAATCGCTACCTAGAACACGTAAACTCGGTGATTCAAGCATCGGACACCTATCTGTTAGATAGCAT
TGGCACCACCATAGCAACCAGTAACTGGAATCTACCGCGTTCTTTTATTCGGCGAAATTTCGCTTTCCGTCCCTACTTTC
AAGAAGCGATTCTTGGCAATGAAAATCAATATTTCGCGCTAGGTTCAACCTCTGGTAAACGAGGCTATTATTATTCTTAC
CCCGTCTCCTATGCCGCCGAGATTATTGGCGTCATTGTCGTAAAGATGGATTTATCGCTGATTGAAGCAAGTTGGAAAGG
CAAACAGAGTTTTTTTGTTGCTGACGATAAAGACCAGATCGTATTTATGTCGAGCAATCCTGAGTGGCTGTTCAAAAGCC
TTCAACCATTAAGCGAAGAACAACACACTCGAATTCAAGAAAGCAGGCAGTATCTCGATACAAAAATAGAAAGCCTGCAC
TTCTCCGGTGACTTAGAGAGTGCTACTAGCAATATTGAGTCTCCCCACAAGCTCGTCCAAGAGCAATTCTTTAACTCCTC
ACGCTTCCTAGCCGAGCCAAAACTCACCATACGAGTATTTTCTCCGACCCACTTAGTTTGGTGGGACTTGGTTGCTTACC
TTGTGGTTTTGAGCCTTATTTTTGCGATTATTTATCTAACGATGCAACTCAATCACCATCGACAACAAAGACGAGCTCAG
ATAGACAGACTGCAATCCGAAGCCAAGCAAAAGCTAGAATTTCAAGTGCTTGAACGAACATCAGAGTTGCATGTCGAGAT
TAAGCACCGAATCGAAACTGAACATGTACTGAGGCAGACACAAGACGAACTCATCCAAGCCGCCAAACTGGCAGTGTTGG
GGCAAATGTCAGCAAGCATCAGCCATGAACTTAATAACCCGCTTGCTGCGATTCGCAGTTACGCCGACAACGGCCGACTG
TTTCTTGCCAAAGAAAAAACCGAGCGTGTCGATGACAACCTATCGCGAATCTCGGCGCTCACTGACCGTATGGCTAAGAT
AAGCCACCAGCTTCGTTCCTTTGCAAAGAAATCCACTGCGGAAGAGCTGCACACGTTACAGATACTGCCAGTATTGCACT
CGTCAAAAGAACTGATGAAGCCACAACTCAAGAGTGAACGAGTTAAGGTCAACGAACTCCCTGAAACCTTTGATGCTTGT
GTGCTCGCCAACGCCATTCAACTGGAGCAAGTGATCATTAACCTGCTGACTAACGCGATTCAGGCGATGGAACAACAAGA
CGACAAACAATTAGCTATTCTGTTAGAGATTAGGAAGCCCGACCATCAACAAGCCAAGACCTTGCTGATTCATGTCGATG
ACAATGGCCCAGGCTTCACCTCCCCTTCTAACGGAAATTTTTTTGAACCCTTCCATACCACCAAGAAAAATGGACTTGGA
CTTGGGCTATCGATTTCTCAACAAATAATCAGCGGAATAAATGGCAAGCTAGTTACCAATCACAGCCCACAAGGTGGTGC
TCGATTTAGTATCGAGTTACCCATAGTTCCAACAAAAGAATCAGATTAG

Upstream 100 bases:

>100_bases
TGGCGACTGTCTACTTTCAGCAAACACTACCGCTATTTGCTACCATAAACGAGAGAAGACAGGCTTGATTATTTACCAAA
GAAATGAGCGGAGAGCCTTC

Downstream 100 bases:

>100_bases
TCGCTAAACAAACCAGTACACCTTCACCAACAATAAAAATAAAAGATTAAAGGAATAACTATGTGTCACGTCTATTTCAT
TGATGATGAATCCGATCTGA

Product: C4-dicarboxylate transport sensor protein DctB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MFQALRIPILLMATYSLLMVFGGHWVWNTSHESLLNDHQSKLDRFSVHISSQLDKFAHIPELLSKDKELVDALHSPSNSA
QIELTNRYLEHVNSVIQASDTYLLDSIGTTIATSNWNLPRSFIRRNFAFRPYFQEAILGNENQYFALGSTSGKRGYYYSY
PVSYAAEIIGVIVVKMDLSLIEASWKGKQSFFVADDKDQIVFMSSNPEWLFKSLQPLSEEQHTRIQESRQYLDTKIESLH
FSGDLESATSNIESPHKLVQEQFFNSSRFLAEPKLTIRVFSPTHLVWWDLVAYLVVLSLIFAIIYLTMQLNHHRQQRRAQ
IDRLQSEAKQKLEFQVLERTSELHVEIKHRIETEHVLRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSYADNGRL
FLAKEKTERVDDNLSRISALTDRMAKISHQLRSFAKKSTAEELHTLQILPVLHSSKELMKPQLKSERVKVNELPETFDAC
VLANAIQLEQVIINLLTNAIQAMEQQDDKQLAILLEIRKPDHQQAKTLLIHVDDNGPGFTSPSNGNFFEPFHTTKKNGLG
LGLSISQQIISGINGKLVTNHSPQGGARFSIELPIVPTKESD

Sequences:

>Translated_602_residues
MFQALRIPILLMATYSLLMVFGGHWVWNTSHESLLNDHQSKLDRFSVHISSQLDKFAHIPELLSKDKELVDALHSPSNSA
QIELTNRYLEHVNSVIQASDTYLLDSIGTTIATSNWNLPRSFIRRNFAFRPYFQEAILGNENQYFALGSTSGKRGYYYSY
PVSYAAEIIGVIVVKMDLSLIEASWKGKQSFFVADDKDQIVFMSSNPEWLFKSLQPLSEEQHTRIQESRQYLDTKIESLH
FSGDLESATSNIESPHKLVQEQFFNSSRFLAEPKLTIRVFSPTHLVWWDLVAYLVVLSLIFAIIYLTMQLNHHRQQRRAQ
IDRLQSEAKQKLEFQVLERTSELHVEIKHRIETEHVLRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSYADNGRL
FLAKEKTERVDDNLSRISALTDRMAKISHQLRSFAKKSTAEELHTLQILPVLHSSKELMKPQLKSERVKVNELPETFDAC
VLANAIQLEQVIINLLTNAIQAMEQQDDKQLAILLEIRKPDHQQAKTLLIHVDDNGPGFTSPSNGNFFEPFHTTKKNGLG
LGLSISQQIISGINGKLVTNHSPQGGARFSIELPIVPTKESD
>Mature_602_residues
MFQALRIPILLMATYSLLMVFGGHWVWNTSHESLLNDHQSKLDRFSVHISSQLDKFAHIPELLSKDKELVDALHSPSNSA
QIELTNRYLEHVNSVIQASDTYLLDSIGTTIATSNWNLPRSFIRRNFAFRPYFQEAILGNENQYFALGSTSGKRGYYYSY
PVSYAAEIIGVIVVKMDLSLIEASWKGKQSFFVADDKDQIVFMSSNPEWLFKSLQPLSEEQHTRIQESRQYLDTKIESLH
FSGDLESATSNIESPHKLVQEQFFNSSRFLAEPKLTIRVFSPTHLVWWDLVAYLVVLSLIFAIIYLTMQLNHHRQQRRAQ
IDRLQSEAKQKLEFQVLERTSELHVEIKHRIETEHVLRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSYADNGRL
FLAKEKTERVDDNLSRISALTDRMAKISHQLRSFAKKSTAEELHTLQILPVLHSSKELMKPQLKSERVKVNELPETFDAC
VLANAIQLEQVIINLLTNAIQAMEQQDDKQLAILLEIRKPDHQQAKTLLIHVDDNGPGFTSPSNGNFFEPFHTTKKNGLG
LGLSISQQIISGINGKLVTNHSPQGGARFSIELPIVPTKESD

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=31.1475409836066, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=26.4227642276423, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=275, Percent_Identity=25.8181818181818, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=273, Percent_Identity=27.1062271062271, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=29.7413793103448, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=27.4900398406374, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68489; Mature: 68489

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQALRIPILLMATYSLLMVFGGHWVWNTSHESLLNDHQSKLDRFSVHISSQLDKFAHIP
CCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLSKDKELVDALHSPSNSAQIELTNRYLEHVNSVIQASDTYLLDSIGTTIATSNWNLPR
HHHHCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCH
SFIRRNFAFRPYFQEAILGNENQYFALGSTSGKRGYYYSYPVSYAAEIIGVIVVKMDLSL
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
IEASWKGKQSFFVADDKDQIVFMSSNPEWLFKSLQPLSEEQHTRIQESRQYLDTKIESLH
HHHCCCCCCEEEEECCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
FSGDLESATSNIESPHKLVQEQFFNSSRFLAEPKLTIRVFSPTHLVWWDLVAYLVVLSLI
CCCCHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
FAIIYLTMQLNHHRQQRRAQIDRLQSEAKQKLEFQVLERTSELHVEIKHRIETEHVLRQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDELIQAAKLAVLGQMSASISHELNNPLAAIRSYADNGRLFLAKEKTERVDDNLSRISAL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHH
TDRMAKISHQLRSFAKKSTAEELHTLQILPVLHSSKELMKPQLKSERVKVNELPETFDAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCEEHHCCHHHHHHH
VLANAIQLEQVIINLLTNAIQAMEQQDDKQLAILLEIRKPDHQQAKTLLIHVDDNGPGFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCC
SPSNGNFFEPFHTTKKNGLGLGLSISQQIISGINGKLVTNHSPQGGARFSIELPIVPTKE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECEECCCC
SD
CC
>Mature Secondary Structure
MFQALRIPILLMATYSLLMVFGGHWVWNTSHESLLNDHQSKLDRFSVHISSQLDKFAHIP
CCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLSKDKELVDALHSPSNSAQIELTNRYLEHVNSVIQASDTYLLDSIGTTIATSNWNLPR
HHHHCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCH
SFIRRNFAFRPYFQEAILGNENQYFALGSTSGKRGYYYSYPVSYAAEIIGVIVVKMDLSL
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
IEASWKGKQSFFVADDKDQIVFMSSNPEWLFKSLQPLSEEQHTRIQESRQYLDTKIESLH
HHHCCCCCCEEEEECCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
FSGDLESATSNIESPHKLVQEQFFNSSRFLAEPKLTIRVFSPTHLVWWDLVAYLVVLSLI
CCCCHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHH
FAIIYLTMQLNHHRQQRRAQIDRLQSEAKQKLEFQVLERTSELHVEIKHRIETEHVLRQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDELIQAAKLAVLGQMSASISHELNNPLAAIRSYADNGRLFLAKEKTERVDDNLSRISAL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHH
TDRMAKISHQLRSFAKKSTAEELHTLQILPVLHSSKELMKPQLKSERVKVNELPETFDAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCEEHHCCHHHHHHH
VLANAIQLEQVIINLLTNAIQAMEQQDDKQLAILLEIRKPDHQQAKTLLIHVDDNGPGFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCC
SPSNGNFFEPFHTTKKNGLGLGLSISQQIISGINGKLVTNHSPQGGARFSIELPIVPTKE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECEECCCC
SD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]