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The map label for this gene is ahpF [H]

Identifier: 218710106

GI number: 218710106

Start: 2286632

End: 2288224

Strand: Direct

Name: ahpF [H]

Synonym: VS_2127

Alternate gene names: 218710106

Gene position: 2286632-2288224 (Clockwise)

Preceding gene: 218710105

Following gene: 218710108

Centisome position: 69.31

GC content: 47.71

Gene sequence:

>1593_bases
ATGTTAGATCAAGCAATGAAGCAGCAGCTAAAAGCATACCTAGAAAACCTAAAAACCAATGTTCAGTTAGTCCTTAGCCT
GGATGGCAGCGACACCGCGAACAAACTTCAAGATCTGGCGAATGATATAGCTTCTCTCACTGACAAAATTGAAGTGATTC
GAGATGATAACGCAAGCACTCGCCGCCCAATCATGCAGGTCGTGAACCAATCGAAAGGCACTGCGATCGGTTTCGCCGGT
TTACCTATGGGTCACGAATTCACGTCACTGGTTCTAGCACTGCTCCATAGTGGTGGTCACCCTATCAAGCTTGAAGCGGA
CGTAATCGAGCAAATTAAAGAACTCGATCAAGAGCTTAATGTAGAGATTTTTATCTCACTATCGTGCCAAAACTGTCCAG
AAGTGGTTCAGGCGTTCAACATGATGTCGGCGATTAACCCTCTAGTTAAGACAACGATGATTGATGGCGCAGCATTCCAA
GACGAAGTGAAGTCTCGCGACATCATGGCAGTACCAAGCGTGTTTATTAACGGTGAGCTATTTGGTCAAGGCCGTATGTC
ACTGGCTGAAATCCTTAACAAAGTCGATTCAGGCGCAGCAGAAAAGAAAGCAGCAAACCTAAACCAACAAGCACCGTTTG
ATGTATTGGTTGTCGGTGGTGGCCCTGCGGGTTCTTCAGCCGCTATCTATGCGGCACGTAAAGGCATTCGTACCGGTGTG
GTTGCTGACCGATTCGGTGGTCAAGTGATGGATACGATGGCGATTGAGAACTTTATCTCAGTGAAAGCGACAACTGGCCC
TAAGTTAGTGGCCAGCCTTGAAGAGCACGTAAAAGAGTACGGTGTTGAAGTGATGACTGAGCAGCGCGCTGCAAACATCA
TTGCGGCAGAAAACACTGAAGACGGTTACATCCACGTAGAGCTAGAGAGCGGCGCAACACTACGAGCTCGCACGGTTATC
ACAAGTACCGGTGCTCGCTGGCGTGAAATGAACGTTCCGGGTGAGCAAGAGTACCGCAATAAAGGTGTAGCTTACTGCCC
ACACTGTGACGGTCCTTTATTCAAAGGTAAGAAAACAGCGGTTATCGGTGGTGGTAACTCAGGCATCGAAGCGGCAATTG
ATTTAGCGGGTATCGTTGAACACGTAACCGTACTTGAATTTGCAGACACACTGCGTGCCGACCAAGTGCTTATCGACAAA
GCGAACGCAACACCAAACATCGAAATCATCAAGATGGCGCAAACAACGCAAGTGATTGGTGATGGCAACCGCGTAACCGG
TCTGGAATACAAAGACCGCAACACCGATGAACTTAAACAGATTGAACTAGCAGGTATCTTTGTTCAAATCGGCCTGATGC
CAAACAGCGAATGGTTGAAGGGTTCGAAGGTAGAGCTTTCACCACGCGGTGAAATTGAAATTAACGCTCATGGCGCAACA
TCAATGAAAGGTGTATTCGCAGCGGGTGACGTAACAACCGTACCTTACAAACAGATCATCATTGCAATGGGTGAAGGTGC
AAAAGCAAGCTTAGGTGCATTTGACCACCTAATCCGTAACTCAGCTCCAGTTAAAGAGGCTGAAACCGCTTAA

Upstream 100 bases:

>100_bases
ACCACAATATTTGCCGACTTGCGCCCACTTTTTATTTCCCTTTTTCAGCAAGAAGGTATTGGTCAATACCAAGACACCGA
ATTTAAGAGCAGGCACGATT

Downstream 100 bases:

>100_bases
GCTTTAAACTTAAAACTTAAAAGCTTCTAGGCATAAAAGCTTTTAACTCAAGTTCCTTAAATATTTGTTAACTTAATTCT
TTACTGTTAACGACTTAGTC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDQAMKQQLKAYLENLKTNVQLVLSLDGSDTANKLQDLANDIASLTDKIEVIRDDNASTRRPIMQVVNQSKGTAIGFAG
LPMGHEFTSLVLALLHSGGHPIKLEADVIEQIKELDQELNVEIFISLSCQNCPEVVQAFNMMSAINPLVKTTMIDGAAFQ
DEVKSRDIMAVPSVFINGELFGQGRMSLAEILNKVDSGAAEKKAANLNQQAPFDVLVVGGGPAGSSAAIYAARKGIRTGV
VADRFGGQVMDTMAIENFISVKATTGPKLVASLEEHVKEYGVEVMTEQRAANIIAAENTEDGYIHVELESGATLRARTVI
TSTGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADTLRADQVLIDK
ANATPNIEIIKMAQTTQVIGDGNRVTGLEYKDRNTDELKQIELAGIFVQIGLMPNSEWLKGSKVELSPRGEIEINAHGAT
SMKGVFAAGDVTTVPYKQIIIAMGEGAKASLGAFDHLIRNSAPVKEAETA

Sequences:

>Translated_530_residues
MLDQAMKQQLKAYLENLKTNVQLVLSLDGSDTANKLQDLANDIASLTDKIEVIRDDNASTRRPIMQVVNQSKGTAIGFAG
LPMGHEFTSLVLALLHSGGHPIKLEADVIEQIKELDQELNVEIFISLSCQNCPEVVQAFNMMSAINPLVKTTMIDGAAFQ
DEVKSRDIMAVPSVFINGELFGQGRMSLAEILNKVDSGAAEKKAANLNQQAPFDVLVVGGGPAGSSAAIYAARKGIRTGV
VADRFGGQVMDTMAIENFISVKATTGPKLVASLEEHVKEYGVEVMTEQRAANIIAAENTEDGYIHVELESGATLRARTVI
TSTGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADTLRADQVLIDK
ANATPNIEIIKMAQTTQVIGDGNRVTGLEYKDRNTDELKQIELAGIFVQIGLMPNSEWLKGSKVELSPRGEIEINAHGAT
SMKGVFAAGDVTTVPYKQIIIAMGEGAKASLGAFDHLIRNSAPVKEAETA
>Mature_530_residues
MLDQAMKQQLKAYLENLKTNVQLVLSLDGSDTANKLQDLANDIASLTDKIEVIRDDNASTRRPIMQVVNQSKGTAIGFAG
LPMGHEFTSLVLALLHSGGHPIKLEADVIEQIKELDQELNVEIFISLSCQNCPEVVQAFNMMSAINPLVKTTMIDGAAFQ
DEVKSRDIMAVPSVFINGELFGQGRMSLAEILNKVDSGAAEKKAANLNQQAPFDVLVVGGGPAGSSAAIYAARKGIRTGV
VADRFGGQVMDTMAIENFISVKATTGPKLVASLEEHVKEYGVEVMTEQRAANIIAAENTEDGYIHVELESGATLRARTVI
TSTGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTAVIGGGNSGIEAAIDLAGIVEHVTVLEFADTLRADQVLIDK
ANATPNIEIIKMAQTTQVIGDGNRVTGLEYKDRNTDELKQIELAGIFVQIGLMPNSEWLKGSKVELSPRGEIEINAHGAT
SMKGVFAAGDVTTVPYKQIIIAMGEGAKASLGAFDHLIRNSAPVKEAETA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=57.8034682080925, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=33.4394904458599, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=32.8025477707006, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321898, Length=322, Percent_Identity=30.1242236024845, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56831; Mature: 56831

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQAMKQQLKAYLENLKTNVQLVLSLDGSDTANKLQDLANDIASLTDKIEVIRDDNAST
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCH
RRPIMQVVNQSKGTAIGFAGLPMGHEFTSLVLALLHSGGHPIKLEADVIEQIKELDQELN
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
VEIFISLSCQNCPEVVQAFNMMSAINPLVKTTMIDGAAFQDEVKSRDIMAVPSVFINGEL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCEEECCEE
FGQGRMSLAEILNKVDSGAAEKKAANLNQQAPFDVLVVGGGPAGSSAAIYAARKGIRTGV
ECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCC
VADRFGGQVMDTMAIENFISVKATTGPKLVASLEEHVKEYGVEVMTEQRAANIIAAENTE
HHHHCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHCCEEEECCCC
DGYIHVELESGATLRARTVITSTGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTA
CCEEEEEECCCCEEEEEEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEE
VIGGGNSGIEAAIDLAGIVEHVTVLEFADTLRADQVLIDKANATPNIEIIKMAQTTQVIG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEHHHEEC
DGNRVTGLEYKDRNTDELKQIELAGIFVQIGLMPNSEWLKGSKVELSPRGEIEINAHGAT
CCCEEECCEECCCCHHHHHHEEEEEEEEEEEECCCCCCCCCCEEEECCCCCEEEECCCCC
SMKGVFAAGDVTTVPYKQIIIAMGEGAKASLGAFDHLIRNSAPVKEAETA
CCCCEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLDQAMKQQLKAYLENLKTNVQLVLSLDGSDTANKLQDLANDIASLTDKIEVIRDDNAST
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCH
RRPIMQVVNQSKGTAIGFAGLPMGHEFTSLVLALLHSGGHPIKLEADVIEQIKELDQELN
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
VEIFISLSCQNCPEVVQAFNMMSAINPLVKTTMIDGAAFQDEVKSRDIMAVPSVFINGEL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCEEECCEE
FGQGRMSLAEILNKVDSGAAEKKAANLNQQAPFDVLVVGGGPAGSSAAIYAARKGIRTGV
ECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCC
VADRFGGQVMDTMAIENFISVKATTGPKLVASLEEHVKEYGVEVMTEQRAANIIAAENTE
HHHHCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHCCEEEECCCC
DGYIHVELESGATLRARTVITSTGARWREMNVPGEQEYRNKGVAYCPHCDGPLFKGKKTA
CCEEEEEECCCCEEEEEEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEE
VIGGGNSGIEAAIDLAGIVEHVTVLEFADTLRADQVLIDKANATPNIEIIKMAQTTQVIG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEHHHEEC
DGNRVTGLEYKDRNTDELKQIELAGIFVQIGLMPNSEWLKGSKVELSPRGEIEINAHGAT
CCCEEECCEECCCCHHHHHHEEEEEEEEEEEECCCCCCCCCCEEEECCCCCEEEECCCCC
SMKGVFAAGDVTTVPYKQIIIAMGEGAKASLGAFDHLIRNSAPVKEAETA
CCCCEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]