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The map label for this gene is hisB [H]

Identifier: 218710004

GI number: 218710004

Start: 2168428

End: 2169516

Strand: Reverse

Name: hisB [H]

Synonym: VS_2023

Alternate gene names: 218710004

Gene position: 2169516-2168428 (Counterclockwise)

Preceding gene: 218710005

Following gene: 218710003

Centisome position: 65.76

GC content: 45.82

Gene sequence:

>1089_bases
GTGAGTAAACAACAGAAAATACTTTTTATAGACCGTGACGGCACCTTAATTGTTGAGCCGCCAGTTGACTTTCAAGTAGA
CCGTTTAGACAAACTAAAATTCGAACCGTTAGTGATTCCTAGCCTACTTGCACTGCAAGATGCGGGTTATCGCTTAGTGA
TGGTGACTAACCAAGATGGTCTAGGTACAGATAGCTACCCGCAAGAAGAGTTCGATGCTCCACACAATATGATGATGGAG
TTCTTTGAGTCTCAGGGCGTTAAGTTTGATGATGTTCTAATCTGCCCTCACTTTGATGAAGACAATTGCTCTTGCCGCAA
GCCTAAGCTGGGTATGGTTAAGGAATACCTACAGGGCGGTAAAGTTGACTTCCAAAAGTCAGTCGTGATTGGTGATCGAA
TGACGGATCTTCAACTTGCTGAGAACATGGCAATTCGTGGTATCCATTACGGACCAGATGCGCAAACTGAAGGCACGCTT
AACTGGCCACAAATCGTTAAAGATCTGACGGTTAATGCGCGCATTGCTGAAGTGGTTCGTACCACTAAAGAAACGGACAT
CAAGGTGGCAGTAAACCTTGATGAAACCGGTGGCAACAAGATCGATACTGGCATGGGTTTCTTCGATCACATGCTTGATC
AAATCGCGACACACGGCGGTTTCCAAATCAACCTGACTGTGAAGGGCGATCTACACATTGATGATCACCACACAGTAGAA
GATACCGCTCTTGCTTTAGGCCAAGCATTGAAAGATGCATTAGGCGATAAACGTGGTATTGGCCGCTTTGGTTTCAGCCT
ACCAATGGATGAGTGTTTGGCTCAGTGTGCGCTAGACCTTTCTGGCCGTCCATACCTGAAGTTCGATGCTAAATTCAGCC
GCGAGCAAGTGGGTGACCTTTCAACAGAGATGGTGGTTCACTTCTTCCGTTCTTTAACCGATACATTGGCTTGTACGCTA
CACCTTTCTTCTGACGGTAATAATGATCACCACATCATTGAGAGCCTATTCAAAGCGTTTGGCCGTACTCTTCGCCAAGC
AATCAAAGTTGAAGGTAACGAGTTACCAAGCAGCAAAGGCGTTCTGTAA

Upstream 100 bases:

>100_bases
GTTTGATTGGATTAATTGCAGGCTGGCAAGTGAAACCAATATTTTGGGTTAGCGTTGATTTTAGCTAGGCCAGCAGATTT
AAAATTAAAAGGAAGTTCAA

Downstream 100 bases:

>100_bases
GGCTAACGTGAGCAAGGAAAAACAATGAAAGATCAAAAAGTCGTTATTATTGATACTGGTTGTGCCAATGTTTCCTCGGT
GAAGTTTGCGATTGAACGTC

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKFEPLVIPSLLALQDAGYRLVMVTNQDGLGTDSYPQEEFDAPHNMMME
FFESQGVKFDDVLICPHFDEDNCSCRKPKLGMVKEYLQGGKVDFQKSVVIGDRMTDLQLAENMAIRGIHYGPDAQTEGTL
NWPQIVKDLTVNARIAEVVRTTKETDIKVAVNLDETGGNKIDTGMGFFDHMLDQIATHGGFQINLTVKGDLHIDDHHTVE
DTALALGQALKDALGDKRGIGRFGFSLPMDECLAQCALDLSGRPYLKFDAKFSREQVGDLSTEMVVHFFRSLTDTLACTL
HLSSDGNNDHHIIESLFKAFGRTLRQAIKVEGNELPSSKGVL

Sequences:

>Translated_362_residues
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKFEPLVIPSLLALQDAGYRLVMVTNQDGLGTDSYPQEEFDAPHNMMME
FFESQGVKFDDVLICPHFDEDNCSCRKPKLGMVKEYLQGGKVDFQKSVVIGDRMTDLQLAENMAIRGIHYGPDAQTEGTL
NWPQIVKDLTVNARIAEVVRTTKETDIKVAVNLDETGGNKIDTGMGFFDHMLDQIATHGGFQINLTVKGDLHIDDHHTVE
DTALALGQALKDALGDKRGIGRFGFSLPMDECLAQCALDLSGRPYLKFDAKFSREQVGDLSTEMVVHFFRSLTDTLACTL
HLSSDGNNDHHIIESLFKAFGRTLRQAIKVEGNELPSSKGVL
>Mature_361_residues
SKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKFEPLVIPSLLALQDAGYRLVMVTNQDGLGTDSYPQEEFDAPHNMMMEF
FESQGVKFDDVLICPHFDEDNCSCRKPKLGMVKEYLQGGKVDFQKSVVIGDRMTDLQLAENMAIRGIHYGPDAQTEGTLN
WPQIVKDLTVNARIAEVVRTTKETDIKVAVNLDETGGNKIDTGMGFFDHMLDQIATHGGFQINLTVKGDLHIDDHHTVED
TALALGQALKDALGDKRGIGRFGFSLPMDECLAQCALDLSGRPYLKFDAKFSREQVGDLSTEMVVHFFRSLTDTLACTLH
LSSDGNNDHHIIESLFKAFGRTLRQAIKVEGNELPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI87082027, Length=358, Percent_Identity=68.7150837988827, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=30.6666666666667, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324776, Length=216, Percent_Identity=42.5925925925926, Blast_Score=162, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568 [H]

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P [H]

EC number: =3.1.3.15; =4.2.1.19 [H]

Molecular weight: Translated: 40320; Mature: 40189

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00954 IGP_DEHYDRATASE_1 ; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKFEPLVIPSLLALQDAGYRLVMVTNQDG
CCCCCEEEEEECCCCEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCC
LGTDSYPQEEFDAPHNMMMEFFESQGVKFDDVLICPHFDEDNCSCRKPKLGMVKEYLQGG
CCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCC
KVDFQKSVVIGDRMTDLQLAENMAIRGIHYGPDAQTEGTLNWPQIVKDLTVNARIAEVVR
CCCEECEEEECCCCHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHH
TTKETDIKVAVNLDETGGNKIDTGMGFFDHMLDQIATHGGFQINLTVKGDLHIDDHHTVE
HCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHH
DTALALGQALKDALGDKRGIGRFGFSLPMDECLAQCALDLSGRPYLKFDAKFSREQVGDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCH
STEMVVHFFRSLTDTLACTLHLSSDGNNDHHIIESLFKAFGRTLRQAIKVEGNELPSSKG
HHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VL
CC
>Mature Secondary Structure 
SKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKFEPLVIPSLLALQDAGYRLVMVTNQDG
CCCCEEEEEECCCCEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCC
LGTDSYPQEEFDAPHNMMMEFFESQGVKFDDVLICPHFDEDNCSCRKPKLGMVKEYLQGG
CCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCC
KVDFQKSVVIGDRMTDLQLAENMAIRGIHYGPDAQTEGTLNWPQIVKDLTVNARIAEVVR
CCCEECEEEECCCCHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHH
TTKETDIKVAVNLDETGGNKIDTGMGFFDHMLDQIATHGGFQINLTVKGDLHIDDHHTVE
HCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHH
DTALALGQALKDALGDKRGIGRFGFSLPMDECLAQCALDLSGRPYLKFDAKFSREQVGDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCH
STEMVVHFFRSLTDTLACTLHLSSDGNNDHHIIESLFKAFGRTLRQAIKVEGNELPSSKG
HHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]