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The map label for this gene is hisH [H]

Identifier: 218710003

GI number: 218710003

Start: 2167789

End: 2168403

Strand: Reverse

Name: hisH [H]

Synonym: VS_2022

Alternate gene names: 218710003

Gene position: 2168403-2167789 (Counterclockwise)

Preceding gene: 218710004

Following gene: 218710002

Centisome position: 65.72

GC content: 44.55

Gene sequence:

>615_bases
ATGAAAGATCAAAAAGTCGTTATTATTGATACTGGTTGTGCCAATGTTTCCTCGGTGAAGTTTGCGATTGAACGTCTAGG
CTACGCAGTTGAAATTTCAAAAGAACCAGACGTTGTTCTTGCTGCCGATAAGCTATTCCTACCCGGTGTAGGTACAGCAA
GTGAGGCAATGAAGAACCTGCAAGAACGTGACCTTGTTACCCTTGTGAAGCAAGTAGAGAAGCCTCTGCTAGGTATCTGT
TTAGGTATGCAGCTGTTAGGCAAATTGTCTCAAGAGAAAGGCCAAAAAGCTGACGAGTTAGTTGAATGCCTAGGATTGTG
TGATGGCGAAGTTCGTTTACTTGAAACGGGTGATTTGCCATTACCACACATGGGTTGGAACACAGTAACCGCGACACCTA
ACCACCCTCTATTCAAAGATATTGAAGAGGGCGAGTACTTCTACTTTGTACACAGCTTCGCAATGTCAGTGGGTGACTAC
ACAATCGCACAATGTGATTACGGCAATCCGTTTACTGCCGCAGTGCAAAGTGGCAACTATTATGGTGTTCAGTTCCACCC
AGAACGTTCGTCAAAAGCTGGCTCTAAGCTGATTCAGAACTTCTTGGAGTTGTAA

Upstream 100 bases:

>100_bases
AGCGTTTGGCCGTACTCTTCGCCAAGCAATCAAAGTTGAAGGTAACGAGTTACCAAGCAGCAAAGGCGTTCTGTAAGGCT
AACGTGAGCAAGGAAAAACA

Downstream 100 bases:

>100_bases
TAAGGATTTGGCAGCGGCTAACGTTGCCCTATAAGGAATGTAAATGATTATTCCCGCGTTAGATTTAATTGAAGGCCAAG
TAGTTCGCTTATTCCAAGGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKDQKVVIIDTGCANVSSVKFAIERLGYAVEISKEPDVVLAADKLFLPGVGTASEAMKNLQERDLVTLVKQVEKPLLGIC
LGMQLLGKLSQEKGQKADELVECLGLCDGEVRLLETGDLPLPHMGWNTVTATPNHPLFKDIEEGEYFYFVHSFAMSVGDY
TIAQCDYGNPFTAAVQSGNYYGVQFHPERSSKAGSKLIQNFLEL

Sequences:

>Translated_204_residues
MKDQKVVIIDTGCANVSSVKFAIERLGYAVEISKEPDVVLAADKLFLPGVGTASEAMKNLQERDLVTLVKQVEKPLLGIC
LGMQLLGKLSQEKGQKADELVECLGLCDGEVRLLETGDLPLPHMGWNTVTATPNHPLFKDIEEGEYFYFVHSFAMSVGDY
TIAQCDYGNPFTAAVQSGNYYGVQFHPERSSKAGSKLIQNFLEL
>Mature_204_residues
MKDQKVVIIDTGCANVSSVKFAIERLGYAVEISKEPDVVLAADKLFLPGVGTASEAMKNLQERDLVTLVKQVEKPLLGIC
LGMQLLGKLSQEKGQKADELVECLGLCDGEVRLLETGDLPLPHMGWNTVTATPNHPLFKDIEEGEYFYFVHSFAMSVGDY
TIAQCDYGNPFTAAVQSGNYYGVQFHPERSSKAGSKLIQNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=59.5, Blast_Score=245, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=34.1121495327103, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22392; Mature: 22392

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQKVVIIDTGCANVSSVKFAIERLGYAVEISKEPDVVLAADKLFLPGVGTASEAMKNL
CCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECCEECCCCCCHHHHHHHH
QERDLVTLVKQVEKPLLGICLGMQLLGKLSQEKGQKADELVECLGLCDGEVRLLETGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCC
LPHMGWNTVTATPNHPLFKDIEEGEYFYFVHSFAMSVGDYTIAQCDYGNPFTAAVQSGNY
CCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCEEEEEECCCCCEEEEEECCCE
YGVQFHPERSSKAGSKLIQNFLEL
EEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKDQKVVIIDTGCANVSSVKFAIERLGYAVEISKEPDVVLAADKLFLPGVGTASEAMKNL
CCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECCEECCCCCCHHHHHHHH
QERDLVTLVKQVEKPLLGICLGMQLLGKLSQEKGQKADELVECLGLCDGEVRLLETGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCC
LPHMGWNTVTATPNHPLFKDIEEGEYFYFVHSFAMSVGDYTIAQCDYGNPFTAAVQSGNY
CCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCEEEEEECCCCCEEEEEECCCE
YGVQFHPERSSKAGSKLIQNFLEL
EEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA