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The map label for this gene is hisI [H]

Identifier: 218710000

GI number: 218710000

Start: 2165606

End: 2166256

Strand: Reverse

Name: hisI [H]

Synonym: VS_2019

Alternate gene names: 218710000

Gene position: 2166256-2165606 (Counterclockwise)

Preceding gene: 218710001

Following gene: 218709995

Centisome position: 65.66

GC content: 47.93

Gene sequence:

>651_bases
ATGAGTTTTGAAACCGCAAGCTTATCAAAAACAGAAGTAGGCGTATTGTCAGAGCGTATTAATTGGGAAAAAGTCGATGG
CTTAGTGCCAGCGATTGTTCAAGATTACCAGTCTAGCCAAGTGCTGATGATGGGATACATGAACCCAGCAGCACTAGAGA
AAACAGGCGAGACTGGCCAAGTGACGTTTTTCTCTCGCACCAAAGAGCGCCTTTGGACGAAAGGTGAAACGTCAGGCAAC
GTATTGCAACTGCACAACATCGCCTTGGACTGTGACAGCGACACATTACTGGTTAAAGTTAATCCCATTGGTCCAACGTG
CCACACAGGTACAACAACGTGTTGGGATGGAGACAAGCAAGAAGAGTCTCAGATGGTGTGGCTTCATCAGCTTGAGCAGC
TATTGGCTGCCCGCAAGGACGCCGATCCTGAATCTTCTTATACTGCCAGCCTATATGCCCGTGGCACCAAGCGTATTTCG
CAAAAAGTGGGCGAAGAAGGCGTTGAAGTCGCGCTTGCGGCGACGTCGGGCGATAAGGCGGAATTAGTGTGTGAATCAGC
AGATTTGATTTACCACTTGATGGTTCTTCTTCAAGACCAAGGCTTATCGATGAACGATGTGGTGAACAAGCTAAAAGAGC
GTCATAAGTAA

Upstream 100 bases:

>100_bases
ACGTGGATGGAGCACTTGCGGCTTCGGTATTCCACAAACAAATCATCAATATTGGTGAACTTAAACAGTATTTAAAACAA
CAAGATGTAGAGGTGCGACT

Downstream 100 bases:

>100_bases
GCTCTCTTTGGATTAATCGAACCACGCAAAATATAGATAAAAGCCCCGTAAACAAATATGTTTACGGGGCTTTTTAACTG
TTTAATTTTCAAGATTTCAA

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSFETASLSKTEVGVLSERINWEKVDGLVPAIVQDYQSSQVLMMGYMNPAALEKTGETGQVTFFSRTKERLWTKGETSGN
VLQLHNIALDCDSDTLLVKVNPIGPTCHTGTTTCWDGDKQEESQMVWLHQLEQLLAARKDADPESSYTASLYARGTKRIS
QKVGEEGVEVALAATSGDKAELVCESADLIYHLMVLLQDQGLSMNDVVNKLKERHK

Sequences:

>Translated_216_residues
MSFETASLSKTEVGVLSERINWEKVDGLVPAIVQDYQSSQVLMMGYMNPAALEKTGETGQVTFFSRTKERLWTKGETSGN
VLQLHNIALDCDSDTLLVKVNPIGPTCHTGTTTCWDGDKQEESQMVWLHQLEQLLAARKDADPESSYTASLYARGTKRIS
QKVGEEGVEVALAATSGDKAELVCESADLIYHLMVLLQDQGLSMNDVVNKLKERHK
>Mature_215_residues
SFETASLSKTEVGVLSERINWEKVDGLVPAIVQDYQSSQVLMMGYMNPAALEKTGETGQVTFFSRTKERLWTKGETSGNV
LQLHNIALDCDSDTLLVKVNPIGPTCHTGTTTCWDGDKQEESQMVWLHQLEQLLAARKDADPESSYTASLYARGTKRISQ
KVGEEGVEVALAATSGDKAELVCESADLIYHLMVLLQDQGLSMNDVVNKLKERHK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=197, Percent_Identity=65.989847715736, Blast_Score=270, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6319819, Length=203, Percent_Identity=34.4827586206897, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 23867; Mature: 23736

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFETASLSKTEVGVLSERINWEKVDGLVPAIVQDYQSSQVLMMGYMNPAALEKTGETGQ
CCCCCCCCCHHHHHHHHHHCCHHHHHCHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCE
VTFFSRTKERLWTKGETSGNVLQLHNIALDCDSDTLLVKVNPIGPTCHTGTTTCWDGDKQ
EEEEECHHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
EESQMVWLHQLEQLLAARKDADPESSYTASLYARGTKRISQKVGEEGVEVALAATSGDKA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
ELVCESADLIYHLMVLLQDQGLSMNDVVNKLKERHK
EEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SFETASLSKTEVGVLSERINWEKVDGLVPAIVQDYQSSQVLMMGYMNPAALEKTGETGQ
CCCCCCCCHHHHHHHHHHCCHHHHHCHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCE
VTFFSRTKERLWTKGETSGNVLQLHNIALDCDSDTLLVKVNPIGPTCHTGTTTCWDGDKQ
EEEEECHHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
EESQMVWLHQLEQLLAARKDADPESSYTASLYARGTKRISQKVGEEGVEVALAATSGDKA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
ELVCESADLIYHLMVLLQDQGLSMNDVVNKLKERHK
EEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]