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The map label for this gene is cysS

Identifier: 218709995

GI number: 218709995

Start: 2160493

End: 2161878

Strand: Reverse

Name: cysS

Synonym: VS_2014

Alternate gene names: 218709995

Gene position: 2161878-2160493 (Counterclockwise)

Preceding gene: 218710000

Following gene: 218709994

Centisome position: 65.53

GC content: 46.03

Gene sequence:

>1386_bases
ATGCTGAAGATATATAACACGCTCACAAGACAGAAAGAGGAATTCAAACCAATTACAGCTGGCAAAGTCGGTATGTATGT
CTGTGGGGTAACCATATACGATCTCTGTCATATTGGTCATGGCCGTACGTTCGTTTCATTTGACGTGGTAACTCGTTACC
TGCGTTACCTTGGTTACGATTTGAATTTCGTTCGTAATATCACTGATATCGATGACAAAATCATCAAGCGCGCTAATGAA
AACGGCGAGTCTTGTGACTCTCTGACTGAGCGTCTGATCGGTGAAATGCACGCGGACTTCGATGCATTGAACATGAAACG
CCCAGATGTCGAGCCTCGTGCGACTGAATTCATCACAGAGATCATTGAGTTAGTAGAAAAGCTAATTCAACGTGGCTTTG
CTTACGTTGCAAGCAACGGTGACGTGATGTTCGAAGTTAAGAAGTTTGACGAATACGGTAAGCTTTCTAAGCAAGATCTT
GATCAGCTTCAAGCGGGTGCTCGTGTTGACATCGATTCTGCAAAACGTAGCCCGTTAGACTTTGTTCTTTGGAAGATGTC
TAAGCCTGGTGAGCCGACGTGGGAATCGCCATGGGGGCCAGGTCGTCCAGGTTGGCACATCGAATGTTCAGCAATGAACT
CGTCTATTCTTGGTAATCACTTCGATATTCATGGTGGTGGTTCAGATCTACAGTTCCCGCACCACGAGAACGAAATCGCA
CAATCTTGCTGTGCACATGGCACCCAGTATGTGAATACTTGGATGCACAGTGGCATGGTGATGGTAGACAGAGAAAAAAT
GTCTAAGTCATTGGGTAACTTCTTCACTATCCGTGACGTACTTCAGCATTACGATGCAGAGACAGTTCGTTACTTCCTGA
TGTCTGGTCACTACCGTAGCCAACTGAACTACAGCGAAGATAACCTAAATCAGGCTCGTGCATCACTTGAACGTCTCTAT
ACATCACTTCGTGGTCTAGACGTTACCGCAGCCCCTGCTGGTGGTGAAGAGTATGTGACTCGTTTCTCTACAGCGATGAA
CGATGATTTCAACACACCTGAAGCTTATTCAGTGCTGTTTGAAATGGCTCGTGAAATCAACCGTATCAAGACTGAAAGCA
TAGAAAAAGCAAGTGCACTAGGTGCGTTGATGCGTGAACTGGCAGACATCATCGGTATTCTTCACCAAGATCCAGAAGCT
TTCTTGCAAGGCGATGCAGCAGGTAACGATGACGAAGTCGCTGAAATCGAAGGCCTAATTAAGCTACGTAATGACTCTCG
CGCTTCGAAGGATTGGGCAAATGCCGATCTTGCACGTGATAAGCTGAACGAGCTAGGTATTGTTCTGGAAGATGGTCCAG
AAGGTACGACTTGGCGTCGTAAGTAA

Upstream 100 bases:

>100_bases
GATGTTTGAGGTAATTCCTGCTTTGGGGGATTACCTATTAAGGTATGACTTGTTATACTGCGATCTTATTTTTGATTAAT
CCATAAAATAGATAGAGATC

Downstream 100 bases:

>100_bases
ATCTTATAAAAAGGGCTGATTTTCAGCCCTTTTTTCTAGCAAAAATTCTCTAATTTCACCTTTTTATCTTCAACTTTTAA
TTAACAGGAATATTTCTGTG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLKIYNTLTRQKEEFKPITAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVTRYLRYLGYDLNFVRNITDIDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMKRPDVEPRATEFITEIIELVEKLIQRGFAYVASNGDVMFEVKKFDEYGKLSKQDL
DQLQAGARVDIDSAKRSPLDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLQFPHHENEIA
QSCCAHGTQYVNTWMHSGMVMVDREKMSKSLGNFFTIRDVLQHYDAETVRYFLMSGHYRSQLNYSEDNLNQARASLERLY
TSLRGLDVTAAPAGGEEYVTRFSTAMNDDFNTPEAYSVLFEMAREINRIKTESIEKASALGALMRELADIIGILHQDPEA
FLQGDAAGNDDEVAEIEGLIKLRNDSRASKDWANADLARDKLNELGIVLEDGPEGTTWRRK

Sequences:

>Translated_461_residues
MLKIYNTLTRQKEEFKPITAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVTRYLRYLGYDLNFVRNITDIDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMKRPDVEPRATEFITEIIELVEKLIQRGFAYVASNGDVMFEVKKFDEYGKLSKQDL
DQLQAGARVDIDSAKRSPLDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLQFPHHENEIA
QSCCAHGTQYVNTWMHSGMVMVDREKMSKSLGNFFTIRDVLQHYDAETVRYFLMSGHYRSQLNYSEDNLNQARASLERLY
TSLRGLDVTAAPAGGEEYVTRFSTAMNDDFNTPEAYSVLFEMAREINRIKTESIEKASALGALMRELADIIGILHQDPEA
FLQGDAAGNDDEVAEIEGLIKLRNDSRASKDWANADLARDKLNELGIVLEDGPEGTTWRRK
>Mature_461_residues
MLKIYNTLTRQKEEFKPITAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVTRYLRYLGYDLNFVRNITDIDDKIIKRANE
NGESCDSLTERLIGEMHADFDALNMKRPDVEPRATEFITEIIELVEKLIQRGFAYVASNGDVMFEVKKFDEYGKLSKQDL
DQLQAGARVDIDSAKRSPLDFVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLQFPHHENEIA
QSCCAHGTQYVNTWMHSGMVMVDREKMSKSLGNFFTIRDVLQHYDAETVRYFLMSGHYRSQLNYSEDNLNQARASLERLY
TSLRGLDVTAAPAGGEEYVTRFSTAMNDDFNTPEAYSVLFEMAREINRIKTESIEKASALGALMRELADIIGILHQDPEA
FLQGDAAGNDDEVAEIEGLIKLRNDSRASKDWANADLARDKLNELGIVLEDGPEGTTWRRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=384, Percent_Identity=39.84375, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=45.4935622317597, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=45.4935622317597, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=45.4935622317597, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=45.4935622317597, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=75.0542299349241, Blast_Score=722, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=247, Percent_Identity=41.7004048582996, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17509795, Length=247, Percent_Identity=41.7004048582996, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324082, Length=358, Percent_Identity=34.9162011173184, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI19922218, Length=499, Percent_Identity=35.6713426853707, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI19922364, Length=323, Percent_Identity=36.5325077399381, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_VIBSL (B7VGZ1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417616.1
- GeneID:   7161570
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2014
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52170; Mature: 52170

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNTLTRQKEEFKPITAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVTRYLRYLGYD
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LNFVRNITDIDDKIIKRANENGESCDSLTERLIGEMHADFDALNMKRPDVEPRATEFITE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHH
IIELVEKLIQRGFAYVASNGDVMFEVKKFDEYGKLSKQDLDQLQAGARVDIDSAKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCHH
FVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCHHHHH
QSCCAHGTQYVNTWMHSGMVMVDREKMSKSLGNFFTIRDVLQHYDAETVRYFLMSGHYRS
HHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCHHH
QLNYSEDNLNQARASLERLYTSLRGLDVTAAPAGGEEYVTRFSTAMNDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EMAREINRIKTESIEKASALGALMRELADIIGILHQDPEAFLQGDAAGNDDEVAEIEGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHH
KLRNDSRASKDWANADLARDKLNELGIVLEDGPEGTTWRRK
HHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYNTLTRQKEEFKPITAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVTRYLRYLGYD
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LNFVRNITDIDDKIIKRANENGESCDSLTERLIGEMHADFDALNMKRPDVEPRATEFITE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHH
IIELVEKLIQRGFAYVASNGDVMFEVKKFDEYGKLSKQDLDQLQAGARVDIDSAKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCHH
FVLWKMSKPGEPTWESPWGPGRPGWHIECSAMNSSILGNHFDIHGGGSDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCHHHHH
QSCCAHGTQYVNTWMHSGMVMVDREKMSKSLGNFFTIRDVLQHYDAETVRYFLMSGHYRS
HHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCHHH
QLNYSEDNLNQARASLERLYTSLRGLDVTAAPAGGEEYVTRFSTAMNDDFNTPEAYSVLF
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EMAREINRIKTESIEKASALGALMRELADIIGILHQDPEAFLQGDAAGNDDEVAEIEGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHH
KLRNDSRASKDWANADLARDKLNELGIVLEDGPEGTTWRRK
HHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA