Click here to switch to the map view.

The map label for this gene is hisF1 [H]

Identifier: 218710001

GI number: 218710001

Start: 2166253

End: 2167026

Strand: Reverse

Name: hisF1 [H]

Synonym: VS_2020

Alternate gene names: 218710001

Gene position: 2167026-2166253 (Counterclockwise)

Preceding gene: 218710002

Following gene: 218710000

Centisome position: 65.68

GC content: 45.35

Gene sequence:

>774_bases
ATGTTGGCAAAGCGAATAATCCCTTGTTTGGATGTCCGTGATGGACAAGTGGTTAAGGGCGTTCAATTCCGTAATCACGA
AATTATTGGTGACATCGTTCCGCTAGCACAACGCTACGCAGAAGAGGGCGCTGATGAATTAGTATTTTATGACATCACCG
CATCAAGTGATGGCCGTGTGGTCGATAAAAGCTGGGTGAAACGTGTAGCAGAAGTGATTGATATTCCTTTCTGTGTGGCT
GGTGGTATTAAGTCTGCGGAAGATGCAGCACGCATTCTTGAGTTTGGTGCAGATAAAGTATCCATCAACTCGCCTGCATT
GGCTAACCCACAGCTAATCACTGACCTAGCTGATAAGTTCGGCGTACAGTGTATCGTTGTCGGTATCGATTCATACTTCG
ATAAAGAGACGGGTAAATATCAGGTTTACCAATTCACAGGCGATGAAGCGCGTACCAAAGCAACCAAGTGGGAAACCAAA
GATTGGGTGCAGGAAGTACAGAAGCGTGGCGCCGGTGAGATCGTGTTAAACATGATGAACCAAGATGGCGTTCGCAACGG
CTATGATATCGACCAGTTAAATATGGTACGTTCGGTGTGTAATGTACCCCTGATTGCATCAGGTGGCGCAGGTGCAATGG
AACACTTTGCTGAAGCTTATAAAAAGACCAACGTGGATGGAGCACTTGCGGCTTCGGTATTCCACAAACAAATCATCAAT
ATTGGTGAACTTAAACAGTATTTAAAACAACAAGATGTAGAGGTGCGACTATGA

Upstream 100 bases:

>100_bases
GCCTAGCTGATATCGAAGCACTTAAAGGCAGCGGCGTTGCGGGTGTGATTGTTGGTCGTGCACTGCTTGATGGCAAGTTC
ACCGCAGAGGAGGCATTTGC

Downstream 100 bases:

>100_bases
GTTTTGAAACCGCAAGCTTATCAAAAACAGAAGTAGGCGTATTGTCAGAGCGTATTAATTGGGAAAAAGTCGATGGCTTA
GTGCCAGCGATTGTTCAAGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVKRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEARTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIDQLNMVRSVCNVPLIASGGAGAMEHFAEAYKKTNVDGALAASVFHKQIIN
IGELKQYLKQQDVEVRL

Sequences:

>Translated_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVKRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEARTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIDQLNMVRSVCNVPLIASGGAGAMEHFAEAYKKTNVDGALAASVFHKQIIN
IGELKQYLKQQDVEVRL
>Mature_257_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVKRVAEVIDIPFCVA
GGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEARTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDIDQLNMVRSVCNVPLIASGGAGAMEHFAEAYKKTNVDGALAASVFHKQIIN
IGELKQYLKQQDVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=84.0466926070039, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.7610062893082, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28388; Mature: 28388

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVKRVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYFDKETGKYQVYQFTGDEARTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECHHHHCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIDQLNMVRSVCNVPLIASGGAGAMEHFAEAYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQDVEVRL
HHHHHHHHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVKRVAEVIDIPFCVAGGIKSAEDAARILEFGADKVSINSPALANPQLITDLADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
GVQCIVVGIDSYFDKETGKYQVYQFTGDEARTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECHHHHCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHH
QDGVRNGYDIDQLNMVRSVCNVPLIASGGAGAMEHFAEAYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQYLKQQDVEVRL
HHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA