Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 218709885

GI number: 218709885

Start: 2038927

End: 2041059

Strand: Reverse

Name: nrdE [H]

Synonym: VS_1898

Alternate gene names: 218709885

Gene position: 2041059-2038927 (Counterclockwise)

Preceding gene: 218709886

Following gene: 218709884

Centisome position: 61.86

GC content: 43.37

Gene sequence:

>2133_bases
ATGGATAACCACTTTGAGAACGAGTCCCTTGACTACCATGCTTTAAACGCAATGTTGAATCTACTTGATGAACAAGGGAC
GATTCAGTTTGAGGCAGATAAGCAAGCGGCTCGACAGTTTTTCTTACAACACGTTAATCAAAATACCGTTTTCTTTCATA
ATTTAGACGAGAAGATCGACTATTTGCTCAGAGAGGGATATTACGAGGCTCAAGTTATTGAGCAATATGATCGCAGTTTT
CTTCATCAAATCTGGGCAAACGCCTATCAAGCCAAATTTCGATTCCGCACGTTCTTAGGTGCTTATAAGTTCTTTACTTC
CTATGCACTAAAAACTCGTGATGGTAAGCGATATTTAGAAAGGTTCGAAGACCGAGTGGTTATGACTGCACTGTTACTGG
CTCGTGGTGACTGTGAATTTGCACTGGCACTAATGGAAGAAATCATTCATCAGCGCTTTCAACCGGCTACGCCCACTTTT
ATTAACTCCGGCAAAAAGAGCCGTGGTGAATTGATCTCCTGCTTTTTGTTGAGAATTGAAGACAATATGGAGAGCATCGG
TCGTGCGATTAACTCATCGTTGCAACTCTCACGTCGTGGCGGTGGTGTTGCTTTACTTCTGACGAATTTAAGAGAGCAAG
GTGCGCCCATTAAGGGGATCTTAAATCAAAGTTCAGGTGTCGTCCCCGTGATGAAATTGCTTGAAGACAGTTTCTCGTAT
GCAAACCAATTGGGGTCTAGGCAAGGCGCGGGGGCTGTCTATCTTAATGTTCACCATCCAGACATCATGCAATTTCTTGA
TACGAAAAGAGAAAATGCCGATGAAAAAATCAGAATTAAGACCTTGAGCCTTGGTGTTGTCATCCCCGATATTACTCTGG
AATTAGCCAAGCAAAACAAAGATATGTACTTGTTTTCTCCCCATGATGTTGAGCGAGTGTATGGCGTACCTTTCTCTGAA
ATTAGCGTGACGGAGAAGTACCATGAAATGGTGGACGACCCGAGGATCCGCAAAGGGAAAATGAGTGCAAGAGGCCTTTT
CCAAACGCTTGCCGAGATCCAATTTGAGTCGGGCTATCCATACATTATGTTCGAAGATACGGTTAATCAGGCTAACCCCA
TTCAGGGGCGTGTAAATATGTCTAACTTGTGTTCTGAAATATTGCAGGTCAACGAGGCGTCAAATTATGATGATGACTTG
AACTATAGTCATGTCGGGCGAGATATTTCGTGCAATTTAGGCTCGGTCAATATTGCTAAAGCCTTGGATGGAGGCCAGTT
ATCTCAAACCGTTGATACCGCAATTCGAGCATTAAATGCAGTGTCAGAGATGAGCGCAATCAACAGTGTTCCGTCGATAA
GAAAGGGCAACGATGAAAGTCATGCTATTGGTTTAGGTCAGATGAACTTGCATGGGTTTTTGGCTAGAGAACGCATTCAC
TATGATTCACCAGAGGCCATTGATTTCACCAGCAGTTATTTTGCCTGTGTTTTATATCACTGTCTCGTGGCTTCCAATAA
GTTGGCACAAGAGAAAAAGAGTACGTTCAAAGGGTTCGAAGATTCTAGTTACAGTGATGGGTCTTTCTTCGACAAATATT
TGCAGCAGGATTTCTCGCCAAAACTGGACGTGGTCCAAGATCTCTTTAGCCGTTTAGATATACGAATACCCACAGTGTCT
GAGTGGAGCGAATTGAAACAACTCGTCATGAGCTCAGGGCTATACAACCGATACTTGCAGGCCATTCCTCCTACAGGCTC
AATTAGCTATATCAACGACTCTACCGCTTCTATTCATCCCGTTACCGCTAAAGTGGAGATAAGAAAAGAGGGTAAAATTG
GGCGAGTTTACTTCCCTGCGCCGTATTTGAATAACTCCAATTTAGAGTACTTCCGTGATGCCTTTTCCATAGGCCCTAAG
GCAATAATCGATGTCTATGCGGCGGCCACTGAACATGTTGATCAAGGCCTTTCATTAACTTTGTTTTTTGACGATGAGGT
AACGACGAGAGGTATCAACAAAGCTCAGATCTACGCATGGAAAAAGAAAATTAAAACGCTTTACTACATCCGAATGCGAC
AGAAAGCATTAGAAGGTACTCAAGTCGAAGGGTGCGTGTCGTGCTCACTGTAA

Upstream 100 bases:

>100_bases
GAAAAATGCAATGTGCCGCTGTTGTACCGTTTCGAACTATCTGGAACCCAGAGCGATATTGATATCGTGACAAATGGGTT
AGAACAATTTTGGAAACATC

Downstream 100 bases:

>100_bases
TAAAGGGATAAACGCAATGAAAAAGATGGAATCCGGCCCCGTTCAAGCGATTAACTGGAATCGTATGATTGATGGCAAAG
ATCTAGAGATTTGGAATCGC

Product: ribonucleotide reductase r1 chain

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MDNHFENESLDYHALNAMLNLLDEQGTIQFEADKQAARQFFLQHVNQNTVFFHNLDEKIDYLLREGYYEAQVIEQYDRSF
LHQIWANAYQAKFRFRTFLGAYKFFTSYALKTRDGKRYLERFEDRVVMTALLLARGDCEFALALMEEIIHQRFQPATPTF
INSGKKSRGELISCFLLRIEDNMESIGRAINSSLQLSRRGGGVALLLTNLREQGAPIKGILNQSSGVVPVMKLLEDSFSY
ANQLGSRQGAGAVYLNVHHPDIMQFLDTKRENADEKIRIKTLSLGVVIPDITLELAKQNKDMYLFSPHDVERVYGVPFSE
ISVTEKYHEMVDDPRIRKGKMSARGLFQTLAEIQFESGYPYIMFEDTVNQANPIQGRVNMSNLCSEILQVNEASNYDDDL
NYSHVGRDISCNLGSVNIAKALDGGQLSQTVDTAIRALNAVSEMSAINSVPSIRKGNDESHAIGLGQMNLHGFLARERIH
YDSPEAIDFTSSYFACVLYHCLVASNKLAQEKKSTFKGFEDSSYSDGSFFDKYLQQDFSPKLDVVQDLFSRLDIRIPTVS
EWSELKQLVMSSGLYNRYLQAIPPTGSISYINDSTASIHPVTAKVEIRKEGKIGRVYFPAPYLNNSNLEYFRDAFSIGPK
AIIDVYAAATEHVDQGLSLTLFFDDEVTTRGINKAQIYAWKKKIKTLYYIRMRQKALEGTQVEGCVSCSL

Sequences:

>Translated_710_residues
MDNHFENESLDYHALNAMLNLLDEQGTIQFEADKQAARQFFLQHVNQNTVFFHNLDEKIDYLLREGYYEAQVIEQYDRSF
LHQIWANAYQAKFRFRTFLGAYKFFTSYALKTRDGKRYLERFEDRVVMTALLLARGDCEFALALMEEIIHQRFQPATPTF
INSGKKSRGELISCFLLRIEDNMESIGRAINSSLQLSRRGGGVALLLTNLREQGAPIKGILNQSSGVVPVMKLLEDSFSY
ANQLGSRQGAGAVYLNVHHPDIMQFLDTKRENADEKIRIKTLSLGVVIPDITLELAKQNKDMYLFSPHDVERVYGVPFSE
ISVTEKYHEMVDDPRIRKGKMSARGLFQTLAEIQFESGYPYIMFEDTVNQANPIQGRVNMSNLCSEILQVNEASNYDDDL
NYSHVGRDISCNLGSVNIAKALDGGQLSQTVDTAIRALNAVSEMSAINSVPSIRKGNDESHAIGLGQMNLHGFLARERIH
YDSPEAIDFTSSYFACVLYHCLVASNKLAQEKKSTFKGFEDSSYSDGSFFDKYLQQDFSPKLDVVQDLFSRLDIRIPTVS
EWSELKQLVMSSGLYNRYLQAIPPTGSISYINDSTASIHPVTAKVEIRKEGKIGRVYFPAPYLNNSNLEYFRDAFSIGPK
AIIDVYAAATEHVDQGLSLTLFFDDEVTTRGINKAQIYAWKKKIKTLYYIRMRQKALEGTQVEGCVSCSL
>Mature_710_residues
MDNHFENESLDYHALNAMLNLLDEQGTIQFEADKQAARQFFLQHVNQNTVFFHNLDEKIDYLLREGYYEAQVIEQYDRSF
LHQIWANAYQAKFRFRTFLGAYKFFTSYALKTRDGKRYLERFEDRVVMTALLLARGDCEFALALMEEIIHQRFQPATPTF
INSGKKSRGELISCFLLRIEDNMESIGRAINSSLQLSRRGGGVALLLTNLREQGAPIKGILNQSSGVVPVMKLLEDSFSY
ANQLGSRQGAGAVYLNVHHPDIMQFLDTKRENADEKIRIKTLSLGVVIPDITLELAKQNKDMYLFSPHDVERVYGVPFSE
ISVTEKYHEMVDDPRIRKGKMSARGLFQTLAEIQFESGYPYIMFEDTVNQANPIQGRVNMSNLCSEILQVNEASNYDDDL
NYSHVGRDISCNLGSVNIAKALDGGQLSQTVDTAIRALNAVSEMSAINSVPSIRKGNDESHAIGLGQMNLHGFLARERIH
YDSPEAIDFTSSYFACVLYHCLVASNKLAQEKKSTFKGFEDSSYSDGSFFDKYLQQDFSPKLDVVQDLFSRLDIRIPTVS
EWSELKQLVMSSGLYNRYLQAIPPTGSISYINDSTASIHPVTAKVEIRKEGKIGRVYFPAPYLNNSNLEYFRDAFSIGPK
AIIDVYAAATEHVDQGLSLTLFFDDEVTTRGINKAQIYAWKKKIKTLYYIRMRQKALEGTQVEGCVSCSL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=25.4573170731707, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1789030, Length=703, Percent_Identity=65.149359886202, Blast_Score=980, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=683, Percent_Identity=24.3045387994143, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=681, Percent_Identity=25.2569750367107, Blast_Score=184, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=659, Percent_Identity=25.7966616084977, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320914, Length=659, Percent_Identity=25.4931714719272, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17352481, Length=645, Percent_Identity=25.4263565891473, Blast_Score=180, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80485; Mature: 80485

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNHFENESLDYHALNAMLNLLDEQGTIQFEADKQAARQFFLQHVNQNTVFFHNLDEKID
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHH
YLLREGYYEAQVIEQYDRSFLHQIWANAYQAKFRFRTFLGAYKFFTSYALKTRDGKRYLE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RFEDRVVMTALLLARGDCEFALALMEEIIHQRFQPATPTFINSGKKSRGELISCFLLRIE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHH
DNMESIGRAINSSLQLSRRGGGVALLLTNLREQGAPIKGILNQSSGVVPVMKLLEDSFSY
HHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHHH
ANQLGSRQGAGAVYLNVHHPDIMQFLDTKRENADEKIRIKTLSLGVVIPDITLELAKQNK
HHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHHCCC
DMYLFSPHDVERVYGVPFSEISVTEKYHEMVDDPRIRKGKMSARGLFQTLAEIQFESGYP
CEEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCC
YIMFEDTVNQANPIQGRVNMSNLCSEILQVNEASNYDDDLNYSHVGRDISCNLGSVNIAK
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEECCCCCEEHHH
ALDGGQLSQTVDTAIRALNAVSEMSAINSVPSIRKGNDESHAIGLGQMNLHGFLARERIH
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHC
YDSPEAIDFTSSYFACVLYHCLVASNKLAQEKKSTFKGFEDSSYSDGSFFDKYLQQDFSP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
KLDVVQDLFSRLDIRIPTVSEWSELKQLVMSSGLYNRYLQAIPPTGSISYINDSTASIHP
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCEEEE
VTAKVEIRKEGKIGRVYFPAPYLNNSNLEYFRDAFSIGPKAIIDVYAAATEHVDQGLSLT
EEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
LFFDDEVTTRGINKAQIYAWKKKIKTLYYIRMRQKALEGTQVEGCVSCSL
EEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MDNHFENESLDYHALNAMLNLLDEQGTIQFEADKQAARQFFLQHVNQNTVFFHNLDEKID
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHH
YLLREGYYEAQVIEQYDRSFLHQIWANAYQAKFRFRTFLGAYKFFTSYALKTRDGKRYLE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RFEDRVVMTALLLARGDCEFALALMEEIIHQRFQPATPTFINSGKKSRGELISCFLLRIE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHH
DNMESIGRAINSSLQLSRRGGGVALLLTNLREQGAPIKGILNQSSGVVPVMKLLEDSFSY
HHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHHH
ANQLGSRQGAGAVYLNVHHPDIMQFLDTKRENADEKIRIKTLSLGVVIPDITLELAKQNK
HHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHHCCC
DMYLFSPHDVERVYGVPFSEISVTEKYHEMVDDPRIRKGKMSARGLFQTLAEIQFESGYP
CEEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCC
YIMFEDTVNQANPIQGRVNMSNLCSEILQVNEASNYDDDLNYSHVGRDISCNLGSVNIAK
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEECCCCCEEHHH
ALDGGQLSQTVDTAIRALNAVSEMSAINSVPSIRKGNDESHAIGLGQMNLHGFLARERIH
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHC
YDSPEAIDFTSSYFACVLYHCLVASNKLAQEKKSTFKGFEDSSYSDGSFFDKYLQQDFSP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
KLDVVQDLFSRLDIRIPTVSEWSELKQLVMSSGLYNRYLQAIPPTGSISYINDSTASIHP
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCEEEE
VTAKVEIRKEGKIGRVYFPAPYLNNSNLEYFRDAFSIGPKAIIDVYAAATEHVDQGLSLT
EEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
LFFDDEVTTRGINKAQIYAWKKKIKTLYYIRMRQKALEGTQVEGCVSCSL
EEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]