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The map label for this gene is nrdF [H]

Identifier: 218709884

GI number: 218709884

Start: 2037939

End: 2038910

Strand: Reverse

Name: nrdF [H]

Synonym: VS_1897

Alternate gene names: 218709884

Gene position: 2038910-2037939 (Counterclockwise)

Preceding gene: 218709885

Following gene: 218709881

Centisome position: 61.8

GC content: 42.8

Gene sequence:

>972_bases
ATGAAAAAGATGGAATCCGGCCCCGTTCAAGCGATTAACTGGAATCGTATGATTGATGGCAAAGATCTAGAGATTTGGAA
TCGCCTGACGGTTAATTTTTGGCTGCCTGAAAAGGTACCTTTATCCAATGATATTCAAACATGGAAGCAACTAACTGAAG
AAGAGCAAACGCTAACGATTAGAGTTTTTACCGGTTTAACGTTACTCGACACGATTCAGAATACGGTGGGCGCGCCAGCC
TTGATGGAAGATGCGAGAACGCCTCATGAAGAAGCGGTATTAACCAACATCGCGTTTATGGAAGCCGTTCATGCTCGTAG
CTACTCTTCGGTCTTCTCAACTTTGTGTACTACGCCGCAAATTGATGAAGCGTTTCGCTGGGCTCAAGAGAACCCTCTGT
TACAGAAAAAAGCACAGATTGTCCTCGATGATTATTTGGCTGAAGGCGATCCGCTAAAGAAAAAAGTGGCCAGTGTTTTT
CTAGAGAGTTTCTTATTCTATTCTGGGTTTTATCTACCCATGCATTGGTCGAGTCGGGCTAAACTGACCAATACAGCGGA
TTTGATCCGTCTGATTATTCGTGATGAGGCGATTCATGGTTACTACATTGGATACAAATTTCAACTCGCTTACCAATCTC
TTAGTGAGGAAGAACAGGCGAGAGTTAAGGATGAAGCTTATTCATTAATGTTCTCTCTTTATGAAATCGAAACACAATAT
ACAGAATCGCTTTATGATCCTGTTGGCTTAACTGAAGATGTGAAGCATTTTCTACATTACAATGCCAACAAAGCATTGAT
GAATCTCGGGTTTGAAGCATTGTTCCCGGATGAGTTGTGTCAGGTTAACCCTGCTATCATGGCCGCCCTGTCGCCCAATG
CTGACGAAAATCATGACTTCTTTTCTGGTTCTGGATCTTCTTATGTCATAGGTAAAGCTGTCGCTACTGAAGACGATGAC
TGGGACTTTTGA

Upstream 100 bases:

>100_bases
ATTAAAACGCTTTACTACATCCGAATGCGACAGAAAGCATTAGAAGGTACTCAAGTCGAAGGGTGCGTGTCGTGCTCACT
GTAATAAAGGGATAAACGCA

Downstream 100 bases:

>100_bases
ACAAATAGCCCGAGTATGAACTCGGGCTTGAAAGAGGATTACTTACCCCTGAATCGACATCATCGTTCTAAGGCCAGCCG
TTTGAAAATTAGGTCACTCA

Product: ribonucleoside-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKKMESGPVQAINWNRMIDGKDLEIWNRLTVNFWLPEKVPLSNDIQTWKQLTEEEQTLTIRVFTGLTLLDTIQNTVGAPA
LMEDARTPHEEAVLTNIAFMEAVHARSYSSVFSTLCTTPQIDEAFRWAQENPLLQKKAQIVLDDYLAEGDPLKKKVASVF
LESFLFYSGFYLPMHWSSRAKLTNTADLIRLIIRDEAIHGYYIGYKFQLAYQSLSEEEQARVKDEAYSLMFSLYEIETQY
TESLYDPVGLTEDVKHFLHYNANKALMNLGFEALFPDELCQVNPAIMAALSPNADENHDFFSGSGSSYVIGKAVATEDDD
WDF

Sequences:

>Translated_323_residues
MKKMESGPVQAINWNRMIDGKDLEIWNRLTVNFWLPEKVPLSNDIQTWKQLTEEEQTLTIRVFTGLTLLDTIQNTVGAPA
LMEDARTPHEEAVLTNIAFMEAVHARSYSSVFSTLCTTPQIDEAFRWAQENPLLQKKAQIVLDDYLAEGDPLKKKVASVF
LESFLFYSGFYLPMHWSSRAKLTNTADLIRLIIRDEAIHGYYIGYKFQLAYQSLSEEEQARVKDEAYSLMFSLYEIETQY
TESLYDPVGLTEDVKHFLHYNANKALMNLGFEALFPDELCQVNPAIMAALSPNADENHDFFSGSGSSYVIGKAVATEDDD
WDF
>Mature_323_residues
MKKMESGPVQAINWNRMIDGKDLEIWNRLTVNFWLPEKVPLSNDIQTWKQLTEEEQTLTIRVFTGLTLLDTIQNTVGAPA
LMEDARTPHEEAVLTNIAFMEAVHARSYSSVFSTLCTTPQIDEAFRWAQENPLLQKKAQIVLDDYLAEGDPLKKKVASVF
LESFLFYSGFYLPMHWSSRAKLTNTADLIRLIIRDEAIHGYYIGYKFQLAYQSLSEEEQARVKDEAYSLMFSLYEIETQY
TESLYDPVGLTEDVKHFLHYNANKALMNLGFEALFPDELCQVNPAIMAALSPNADENHDFFSGSGSSYVIGKAVATEDDD
WDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=262, Percent_Identity=24.4274809160305, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI289177074, Length=262, Percent_Identity=24.4274809160305, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789031, Length=320, Percent_Identity=68.4375, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788567, Length=281, Percent_Identity=26.3345195729537, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36891; Mature: 36891

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMESGPVQAINWNRMIDGKDLEIWNRLTVNFWLPEKVPLSNDIQTWKQLTEEEQTLTI
CCCCCCCCCEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHCCHHEEEE
RVFTGLTLLDTIQNTVGAPALMEDARTPHEEAVLTNIAFMEAVHARSYSSVFSTLCTTPQ
EEECCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDEAFRWAQENPLLQKKAQIVLDDYLAEGDPLKKKVASVFLESFLFYSGFYLPMHWSSRA
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KLTNTADLIRLIIRDEAIHGYYIGYKFQLAYQSLSEEEQARVKDEAYSLMFSLYEIETQY
CCCHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TESLYDPVGLTEDVKHFLHYNANKALMNLGFEALFPDELCQVNPAIMAALSPNADENHDF
HHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCHHCCCHHHHHCCHHHEEEECCCCCCCCCC
FSGSGSSYVIGKAVATEDDDWDF
CCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKKMESGPVQAINWNRMIDGKDLEIWNRLTVNFWLPEKVPLSNDIQTWKQLTEEEQTLTI
CCCCCCCCCEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHCCHHEEEE
RVFTGLTLLDTIQNTVGAPALMEDARTPHEEAVLTNIAFMEAVHARSYSSVFSTLCTTPQ
EEECCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDEAFRWAQENPLLQKKAQIVLDDYLAEGDPLKKKVASVFLESFLFYSGFYLPMHWSSRA
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KLTNTADLIRLIIRDEAIHGYYIGYKFQLAYQSLSEEEQARVKDEAYSLMFSLYEIETQY
CCCHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
TESLYDPVGLTEDVKHFLHYNANKALMNLGFEALFPDELCQVNPAIMAALSPNADENHDF
HHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCHHCCCHHHHHCCHHHEEEECCCCCCCCCC
FSGSGSSYVIGKAVATEDDDWDF
CCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195103; 11677609; 2691838; 2548994; 9748343 [H]