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The map label for this gene is rlmI [H]

Identifier: 218709386

GI number: 218709386

Start: 1525694

End: 1526887

Strand: Reverse

Name: rlmI [H]

Synonym: VS_1395

Alternate gene names: 218709386

Gene position: 1526887-1525694 (Counterclockwise)

Preceding gene: 218709387

Following gene: 218709384

Centisome position: 46.28

GC content: 43.55

Gene sequence:

>1194_bases
ATGACTCCTTCAATTCATCTAGCTAAAGGCCGAGATAAATCACTACGCCGTAAACACCCTTGGGTATTTTCACGCGGTAT
CGATAAGGTCGAAGGCGAACCACAACAAGGTGAAACTGTAGACGTATATGCTCAGAATGGTCAATGGCTAGCAAAAGCGG
CTTACTCACCAGAATCTCAAATTCGAGCTCGTGTTTGGACATTTGAAAAAGAAGAAATTAACAAAGCGTTCTTCGTAAAA
CGAATTCAAGACGCGCAATCACTGCGTGAAGACATCATTGAGCGTGATGGTTTAACTGGCTACCGCCTTACCGCTGCAGA
GTCTGATGGACTGCCAGGTATCACTATCGACAAATATCAAGACTACCTAGTTTGCCAACTACTAAGTGCGGGCGCTGAAT
TCAATAAGAGCGTATTGGTTGAAGCTTTAATTGAATGCTTCCCAGACTGTAATATTTACGAGCGTTCAGACGTAGCAGTT
CGTAAAAAAGAAGGCCTAGAACAAGTTGTTGGTGTACTTCACGGTGAAGAGCCACCAAAATCTATCGTCATTGAAGAAAA
CGGCGTCAAAATCAGCGTAAACATCATGGAAGGCCACAAAACTGGCTTCTACATGGACCAACGTGATAGCCGTAAAGAGT
CAATGAAGTATGTTAAAGGCAAAGATGTGCTTAACTGTTTCTCATACACTGGCGGCTTTGGTCTTTACGCACTGAAAGGC
GACGCAAAACGTGTAATCAACGCAGATGTGTCTCAATTAGCTCTTGATACAGCTAAGTTCAACGCTGAACTCAATGAATT
TGATATCTCGAAAAAGCGTGCTGTATTCCTAAACGCAGACGTATTTAAACTACTTCGTGAATACCGAGATCAAGGCACTA
AGTTTGACGTCGTGATCATGGACCCACCAAAGTTCGTTTCAAGTAAAAACAACCTAACATCAGGTGCTAACGGCTACAAA
GATGTAAACATGCTTGCAATGCAAATCCTAAATCCTGGTGGCACTCTACTCACCTACTCTTGCTCTGGCTTGATGGGCAC
TGATTTATTCCAAAAAATCATCGCTGACGCAGCTCTAGACGCTGGCAGAACAGTAAAATTTGTCGAGCGCTTCGAGCAAG
CAGCCGATCACCTTACAGACACCGCCTACCCAGAAGGTTTCTATCTGAAAGGTTTTGCCTGTAAAGTGTTGTAA

Upstream 100 bases:

>100_bases
GTTCAATACAAGGGCTGCTTTTGCAGCCCTTTCTTATTTGCTTTTCCCTTGCACTTCCCCGACAATACCCCTCCTTCAAA
TTCAACAAACAGAGTAAATT

Downstream 100 bases:

>100_bases
CCGAAGCGTGTTATAACTTCAATGTAAGGTCTTCTTCAGCGCGGTTACTTTACTAAATTAAGATATAAAAAAAGGGCCTT
TCGGCCCTTTTTGTTTAGTT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTPSIHLAKGRDKSLRRKHPWVFSRGIDKVEGEPQQGETVDVYAQNGQWLAKAAYSPESQIRARVWTFEKEEINKAFFVK
RIQDAQSLREDIIERDGLTGYRLTAAESDGLPGITIDKYQDYLVCQLLSAGAEFNKSVLVEALIECFPDCNIYERSDVAV
RKKEGLEQVVGVLHGEEPPKSIVIEENGVKISVNIMEGHKTGFYMDQRDSRKESMKYVKGKDVLNCFSYTGGFGLYALKG
DAKRVINADVSQLALDTAKFNAELNEFDISKKRAVFLNADVFKLLREYRDQGTKFDVVIMDPPKFVSSKNNLTSGANGYK
DVNMLAMQILNPGGTLLTYSCSGLMGTDLFQKIIADAALDAGRTVKFVERFEQAADHLTDTAYPEGFYLKGFACKVL

Sequences:

>Translated_397_residues
MTPSIHLAKGRDKSLRRKHPWVFSRGIDKVEGEPQQGETVDVYAQNGQWLAKAAYSPESQIRARVWTFEKEEINKAFFVK
RIQDAQSLREDIIERDGLTGYRLTAAESDGLPGITIDKYQDYLVCQLLSAGAEFNKSVLVEALIECFPDCNIYERSDVAV
RKKEGLEQVVGVLHGEEPPKSIVIEENGVKISVNIMEGHKTGFYMDQRDSRKESMKYVKGKDVLNCFSYTGGFGLYALKG
DAKRVINADVSQLALDTAKFNAELNEFDISKKRAVFLNADVFKLLREYRDQGTKFDVVIMDPPKFVSSKNNLTSGANGYK
DVNMLAMQILNPGGTLLTYSCSGLMGTDLFQKIIADAALDAGRTVKFVERFEQAADHLTDTAYPEGFYLKGFACKVL
>Mature_396_residues
TPSIHLAKGRDKSLRRKHPWVFSRGIDKVEGEPQQGETVDVYAQNGQWLAKAAYSPESQIRARVWTFEKEEINKAFFVKR
IQDAQSLREDIIERDGLTGYRLTAAESDGLPGITIDKYQDYLVCQLLSAGAEFNKSVLVEALIECFPDCNIYERSDVAVR
KKEGLEQVVGVLHGEEPPKSIVIEENGVKISVNIMEGHKTGFYMDQRDSRKESMKYVKGKDVLNCFSYTGGFGLYALKGD
AKRVINADVSQLALDTAKFNAELNEFDISKKRAVFLNADVFKLLREYRDQGTKFDVVIMDPPKFVSSKNNLTSGANGYKD
VNMLAMQILNPGGTLLTYSCSGLMGTDLFQKIIADAALDAGRTVKFVERFEQAADHLTDTAYPEGFYLKGFACKVL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=398, Percent_Identity=59.5477386934673, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1787181, Length=228, Percent_Identity=28.0701754385965, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44430; Mature: 44299

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSIHLAKGRDKSLRRKHPWVFSRGIDKVEGEPQQGETVDVYAQNGQWLAKAAYSPESQ
CCCCEEEECCCCHHHHHCCCCHHHCCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHH
IRARVWTFEKEEINKAFFVKRIQDAQSLREDIIERDGLTGYRLTAAESDGLPGITIDKYQ
HHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
DYLVCQLLSAGAEFNKSVLVEALIECFPDCNIYERSDVAVRKKEGLEQVVGVLHGEEPPK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCC
SIVIEENGVKISVNIMEGHKTGFYMDQRDSRKESMKYVKGKDVLNCFSYTGGFGLYALKG
EEEEECCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEECC
DAKRVINADVSQLALDTAKFNAELNEFDISKKRAVFLNADVFKLLREYRDQGTKFDVVIM
CHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFVSSKNNLTSGANGYKDVNMLAMQILNPGGTLLTYSCSGLMGTDLFQKIIADAALD
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
AGRTVKFVERFEQAADHLTDTAYPEGFYLKGFACKVL
CCCHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECC
>Mature Secondary Structure 
TPSIHLAKGRDKSLRRKHPWVFSRGIDKVEGEPQQGETVDVYAQNGQWLAKAAYSPESQ
CCCEEEECCCCHHHHHCCCCHHHCCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCHHH
IRARVWTFEKEEINKAFFVKRIQDAQSLREDIIERDGLTGYRLTAAESDGLPGITIDKYQ
HHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
DYLVCQLLSAGAEFNKSVLVEALIECFPDCNIYERSDVAVRKKEGLEQVVGVLHGEEPPK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCC
SIVIEENGVKISVNIMEGHKTGFYMDQRDSRKESMKYVKGKDVLNCFSYTGGFGLYALKG
EEEEECCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEECC
DAKRVINADVSQLALDTAKFNAELNEFDISKKRAVFLNADVFKLLREYRDQGTKFDVVIM
CHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFVSSKNNLTSGANGYKDVNMLAMQILNPGGTLLTYSCSGLMGTDLFQKIIADAALD
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
AGRTVKFVERFEQAADHLTDTAYPEGFYLKGFACKVL
CCCHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA