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The map label for this gene is dagA [H]

Identifier: 218709287

GI number: 218709287

Start: 1391902

End: 1393443

Strand: Reverse

Name: dagA [H]

Synonym: VS_1296

Alternate gene names: 218709287

Gene position: 1393443-1391902 (Counterclockwise)

Preceding gene: 218709288

Following gene: 218709284

Centisome position: 42.23

GC content: 43.45

Gene sequence:

>1542_bases
ATGAAAAACATACGTGAATTATTTTTTGGGTTTATATTATTATTTAGTTCCAGTGCATTTGCAGAAGAAGGATCATTTAA
TCAAGCTATCAGTAACTTAAGTCAAAGTGTTGATGGTTTTTTCAACGAATATACAGGTTGGTTCGTTGGACTGATATTTA
AAAGCGTACCGGTAGGTGAAGCAAACTTCCCAATTATTGTTGGCTGGTTACTGCTCGCTGCGATCATCTTTACTGTTTAC
TTTGGCTTTGTTCAATTTAAACGTGTCGGGATGGCCATAGACATTATTAAAGGTAAGTACACAGACCCTAACTCCAAACA
CGAAGGGGAAGTTTCTCACTTTCAAGCGTTAACAACCGCGCTTTCTGGAACTGTTGGGCTTGGTAATATTGCCGGTGTGG
GTGCTGCACTCGCAATTGGTGGTCCTGGTGCAACATTCTGGATGATCTTGTGTGGCCTTCTGGGTATGGCGTCTAAGTTC
TGTGAGTGTACCTTAGGTGTGAAATACCGAACTATCCTACCTTCAGGTGTCGTTTCCGGTGGTCCCATGTACTACCTAAG
CCAAGGTCTTGGTGAAAGAGGGTTGGGTGGACTAGGTAAAGCCCTTGCTATTGGTTTTGCATTAATGTGTATTTTAGGCG
CATTGGGCGGCGGTAACATGTTCCAAGCTAACCAAGCACATGCCATGTTAACTTACGCATTCGATGTACCAAGTGAATAC
GGTGTCATCACAGGCCTTGTACTCGCCGCTTTAGTTTTCTCTGTGATTGTTGGTGGTATGCCTTCTATCGCATCGGTAAC
GGAAAAAGTTGTTCCTTGGATGGCTGCTCTGTATATCGGTATGGCTCTGATTGTTATCGGTTCTAATCTTGATCAAGTGG
GTCCTGCGTTCAGTGCAATCTTTACTGGTGCGTTTACTGGCGAAGGTGTGGTTGGTGGATTCATTGGTGCCTTAATCCAA
GGTCTAAAACGTGCAACGTTCTCAAACGAAGCAGGTGTAGGTTCAGCAGCTATCGCTCACTCAGCGGTTAAAACAAAAGA
GCCAATCACTGAAGGTCTAGTATCACTATTAGAACCGTTCGTTGATACAGTCATCATTTGTACAATGACAGCCTTGGTTA
TCACTATCGCTGGTCTAAATACAGGTCCATTCGATGGTTCTGGCTTAACGGGTGTAACGCTTACTGCTGCATCGTTTACT
GAAACGGCAGAAGTGTTCAAATACACACTCGCTCTAGCGGTAATCATGTTCGCGTTTTCTACCATGATCTCTTGGTCGTA
CTACGGTCTTAAGGCTTGGACTTACCTATTTGGTGAAGGCAAAACAACGGAACTGATTTTTAAAGTGATGTTTTGTGTGT
TTGTTGTTATCGGTGCGACGATTCAGTTTGGCGCGGTAATCGACTTCTCTGACGCAGCTATCTTTGCGATGTCTATCTTC
AACATTCTTGGCCTGTACTTCCTAATGCCTGTTGTGAAGAAAGAACTGCAATCATTTGTTGCTCGCGTTAAATCTGGCGA
AATCAAAACTTACGAAAAATAG

Upstream 100 bases:

>100_bases
TGCCGTCGAATTGCAATAAATAAGTCGCTGAAATGTAAAAAATCAAATAGAAAAAACACAACAGCAACAAACATAATTTA
ATAAAAAGACAGGGAAAACC

Downstream 100 bases:

>100_bases
TGACTCGTCAATAATTTATTGTCGAATAACACTGTTCTAAAAATTTTTGTAAGACCCTTTGCTTGAGAAAGCGAGAACAG
TATTGCAACGTACAAAATCC

Product: sodium:alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKNIRELFFGFILLFSSSAFAEEGSFNQAISNLSQSVDGFFNEYTGWFVGLIFKSVPVGEANFPIIVGWLLLAAIIFTVY
FGFVQFKRVGMAIDIIKGKYTDPNSKHEGEVSHFQALTTALSGTVGLGNIAGVGAALAIGGPGATFWMILCGLLGMASKF
CECTLGVKYRTILPSGVVSGGPMYYLSQGLGERGLGGLGKALAIGFALMCILGALGGGNMFQANQAHAMLTYAFDVPSEY
GVITGLVLAALVFSVIVGGMPSIASVTEKVVPWMAALYIGMALIVIGSNLDQVGPAFSAIFTGAFTGEGVVGGFIGALIQ
GLKRATFSNEAGVGSAAIAHSAVKTKEPITEGLVSLLEPFVDTVIICTMTALVITIAGLNTGPFDGSGLTGVTLTAASFT
ETAEVFKYTLALAVIMFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKVMFCVFVVIGATIQFGAVIDFSDAAIFAMSIF
NILGLYFLMPVVKKELQSFVARVKSGEIKTYEK

Sequences:

>Translated_513_residues
MKNIRELFFGFILLFSSSAFAEEGSFNQAISNLSQSVDGFFNEYTGWFVGLIFKSVPVGEANFPIIVGWLLLAAIIFTVY
FGFVQFKRVGMAIDIIKGKYTDPNSKHEGEVSHFQALTTALSGTVGLGNIAGVGAALAIGGPGATFWMILCGLLGMASKF
CECTLGVKYRTILPSGVVSGGPMYYLSQGLGERGLGGLGKALAIGFALMCILGALGGGNMFQANQAHAMLTYAFDVPSEY
GVITGLVLAALVFSVIVGGMPSIASVTEKVVPWMAALYIGMALIVIGSNLDQVGPAFSAIFTGAFTGEGVVGGFIGALIQ
GLKRATFSNEAGVGSAAIAHSAVKTKEPITEGLVSLLEPFVDTVIICTMTALVITIAGLNTGPFDGSGLTGVTLTAASFT
ETAEVFKYTLALAVIMFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKVMFCVFVVIGATIQFGAVIDFSDAAIFAMSIF
NILGLYFLMPVVKKELQSFVARVKSGEIKTYEK
>Mature_513_residues
MKNIRELFFGFILLFSSSAFAEEGSFNQAISNLSQSVDGFFNEYTGWFVGLIFKSVPVGEANFPIIVGWLLLAAIIFTVY
FGFVQFKRVGMAIDIIKGKYTDPNSKHEGEVSHFQALTTALSGTVGLGNIAGVGAALAIGGPGATFWMILCGLLGMASKF
CECTLGVKYRTILPSGVVSGGPMYYLSQGLGERGLGGLGKALAIGFALMCILGALGGGNMFQANQAHAMLTYAFDVPSEY
GVITGLVLAALVFSVIVGGMPSIASVTEKVVPWMAALYIGMALIVIGSNLDQVGPAFSAIFTGAFTGEGVVGGFIGALIQ
GLKRATFSNEAGVGSAAIAHSAVKTKEPITEGLVSLLEPFVDTVIICTMTALVITIAGLNTGPFDGSGLTGVTLTAASFT
ETAEVFKYTLALAVIMFAFSTMISWSYYGLKAWTYLFGEGKTTELIFKVMFCVFVVIGATIQFGAVIDFSDAAIFAMSIF
NILGLYFLMPVVKKELQSFVARVKSGEIKTYEK

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=450, Percent_Identity=31.5555555555556, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 54141; Mature: 54141

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRELFFGFILLFSSSAFAEEGSFNQAISNLSQSVDGFFNEYTGWFVGLIFKSVPVGE
CCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ANFPIIVGWLLLAAIIFTVYFGFVQFKRVGMAIDIIKGKYTDPNSKHEGEVSHFQALTTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LSGTVGLGNIAGVGAALAIGGPGATFWMILCGLLGMASKFCECTLGVKYRTILPSGVVSG
HHCCCCCCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GPMYYLSQGLGERGLGGLGKALAIGFALMCILGALGGGNMFQANQAHAMLTYAFDVPSEY
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECCCCCC
GVITGLVLAALVFSVIVGGMPSIASVTEKVVPWMAALYIGMALIVIGSNLDQVGPAFSAI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FTGAFTGEGVVGGFIGALIQGLKRATFSNEAGVGSAAIAHSAVKTKEPITEGLVSLLEPF
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
VDTVIICTMTALVITIAGLNTGPFDGSGLTGVTLTAASFTETAEVFKYTLALAVIMFAFS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TMISWSYYGLKAWTYLFGEGKTTELIFKVMFCVFVVIGATIQFGAVIDFSDAAIFAMSIF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
NILGLYFLMPVVKKELQSFVARVKSGEIKTYEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKNIRELFFGFILLFSSSAFAEEGSFNQAISNLSQSVDGFFNEYTGWFVGLIFKSVPVGE
CCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ANFPIIVGWLLLAAIIFTVYFGFVQFKRVGMAIDIIKGKYTDPNSKHEGEVSHFQALTTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LSGTVGLGNIAGVGAALAIGGPGATFWMILCGLLGMASKFCECTLGVKYRTILPSGVVSG
HHCCCCCCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GPMYYLSQGLGERGLGGLGKALAIGFALMCILGALGGGNMFQANQAHAMLTYAFDVPSEY
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECCCCCC
GVITGLVLAALVFSVIVGGMPSIASVTEKVVPWMAALYIGMALIVIGSNLDQVGPAFSAI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FTGAFTGEGVVGGFIGALIQGLKRATFSNEAGVGSAAIAHSAVKTKEPITEGLVSLLEPF
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHH
VDTVIICTMTALVITIAGLNTGPFDGSGLTGVTLTAASFTETAEVFKYTLALAVIMFAFS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TMISWSYYGLKAWTYLFGEGKTTELIFKVMFCVFVVIGATIQFGAVIDFSDAAIFAMSIF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
NILGLYFLMPVVKKELQSFVARVKSGEIKTYEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]