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The map label for this gene is pflA [H]

Identifier: 218709044

GI number: 218709044

Start: 1098239

End: 1098979

Strand: Reverse

Name: pflA [H]

Synonym: VS_1050

Alternate gene names: 218709044

Gene position: 1098979-1098239 (Counterclockwise)

Preceding gene: 218709045

Following gene: 218709043

Centisome position: 33.31

GC content: 43.59

Gene sequence:

>741_bases
ATGTCTACAACTGGTCGTATTCACTCATTCGAATCTTGTGGTACTGTCGATGGCCCTGGTATCCGCTTTATTGTGTTTCT
TCAAGGCTGCTTAATGCGTTGTATGTACTGCCATAATCGCGATACATGGGAGCTTCATGACGGAAAGGAAGTAACGGTCG
AAGAGATCATTAACGAAGCAAAATCATACCGTCATTTCATGAAAGCCTCTGGCGGTGGTATCACCTGTTCAGGTGGCGAA
GCGATGCTACAACCTGAGTTTGTTCGTGATTTTTTCCGCGCGGCTCAAGCTGAAGGCATCCACACTTGTCTTGATACTAA
TGGCTATATTCGTAAACACACTGAAGTGGTCGATGAAGTACTAGAAGCCTCTGATCTGGTAATGCTGGATCTTAAACATA
TGCGAGATGAAATTCACCACGATTTCATTGGTGTATCAAACCGACGTACTCTGGATTTTGCACGCTACCTACACAAAATA
GGTAAGAAGACGTGGATTCGTTATGTGATTGTTCCTGGCTACACAGATACGCCAGAAGATGCGCATCTTCTGGGTGAATT
CATTAAGGACATGGATAACATCGAAAAAGTAGAACTGCTTCCATACCACAAGCTAGGTGCTCATAAGTGGGAAGCATTGG
GTCACGACTACCCTCTTGAAGGTACAAATCCGCCAAGCAAAGAAAAAATGGACGAGATTGTTGCTATCCTCAGTCAATAC
CATTCAAACGTAAAATACTAA

Upstream 100 bases:

>100_bases
ATTGCCTTTATTTTTAAAGACCTAGTTACCATTTGACCCTATTTTATGTACCGTTTTTGTAATAAAATAAACGGTATAAA
TTCCTATACGAGAATAGCTC

Downstream 100 bases:

>100_bases
TTTAGACTTACGTTAATAGTGATTTCAAACGCCTCAATTTCGATTGGGGCGTTTTCTTTTTTGGTTAAAACAATCCTTTC
ACTTTTCTTTACAACTTTTG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSTTGRIHSFESCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWELHDGKEVTVEEIINEAKSYRHFMKASGGGITCSGGE
AMLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTEVVDEVLEASDLVMLDLKHMRDEIHHDFIGVSNRRTLDFARYLHKI
GKKTWIRYVIVPGYTDTPEDAHLLGEFIKDMDNIEKVELLPYHKLGAHKWEALGHDYPLEGTNPPSKEKMDEIVAILSQY
HSNVKY

Sequences:

>Translated_246_residues
MSTTGRIHSFESCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWELHDGKEVTVEEIINEAKSYRHFMKASGGGITCSGGE
AMLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTEVVDEVLEASDLVMLDLKHMRDEIHHDFIGVSNRRTLDFARYLHKI
GKKTWIRYVIVPGYTDTPEDAHLLGEFIKDMDNIEKVELLPYHKLGAHKWEALGHDYPLEGTNPPSKEKMDEIVAILSQY
HSNVKY
>Mature_245_residues
STTGRIHSFESCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWELHDGKEVTVEEIINEAKSYRHFMKASGGGITCSGGEA
MLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTEVVDEVLEASDLVMLDLKHMRDEIHHDFIGVSNRRTLDFARYLHKIG
KKTWIRYVIVPGYTDTPEDAHLLGEFIKDMDNIEKVELLPYHKLGAHKWEALGHDYPLEGTNPPSKEKMDEIVAILSQYH
SNVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=65.4471544715447, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1790389, Length=253, Percent_Identity=25.6916996047431, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI226510931, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28174; Mature: 28043

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTGRIHSFESCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWELHDGKEVTVEEIINEA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
KSYRHFMKASGGGITCSGGEAMLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTEVVDEV
HHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHH
LEASDLVMLDLKHMRDEIHHDFIGVSNRRTLDFARYLHKIGKKTWIRYVIVPGYTDTPED
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
AHLLGEFIKDMDNIEKVELLPYHKLGAHKWEALGHDYPLEGTNPPSKEKMDEIVAILSQY
HHHHHHHHHHHHCCCHHHCCCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HSNVKY
HHCCCC
>Mature Secondary Structure 
STTGRIHSFESCGTVDGPGIRFIVFLQGCLMRCMYCHNRDTWELHDGKEVTVEEIINEA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
KSYRHFMKASGGGITCSGGEAMLQPEFVRDFFRAAQAEGIHTCLDTNGYIRKHTEVVDEV
HHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHH
LEASDLVMLDLKHMRDEIHHDFIGVSNRRTLDFARYLHKIGKKTWIRYVIVPGYTDTPED
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
AHLLGEFIKDMDNIEKVELLPYHKLGAHKWEALGHDYPLEGTNPPSKEKMDEIVAILSQY
HHHHHHHHHHHHCCCHHHCCCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HSNVKY
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]