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The map label for this gene is rnfA [H]

Identifier: 218708974

GI number: 218708974

Start: 1011240

End: 1011818

Strand: Reverse

Name: rnfA [H]

Synonym: VS_0978

Alternate gene names: 218708974

Gene position: 1011818-1011240 (Counterclockwise)

Preceding gene: 218708978

Following gene: 218708973

Centisome position: 30.67

GC content: 46.63

Gene sequence:

>579_bases
ATGACCGAATACCTTTTGTTGTTGGTTGGCACAGTGCTGGTCAATAACTTTGTGCTAGTGAAGTTTTTAGGGCTATGTCC
TTTCATGGGAGTCTCCAAGAAACTGGAGACTGCGATTGGCATGGGCCTCGCGACGACTTTCGTTCTGACGTTAGCGTCGG
TATCTGCATACCTAGTAGAAACCTACATCCTTACCCCTCTGGGTATTGAATACCTGCGTACCATGAGCTTTATCTTGGTT
ATCGCGGTGGTTGTTCAATTTACGGAAATGGTCGTTCACAAAACCAGCCCAACACTATATCGCCTATTGGGGATCTTTCT
GCCGCTTATCACCACCAACTGTGCGGTATTAGGTGTGGCACTTCTGAACATCAATGAAAACCACAACTTCATTCAGTCAA
TCATCTATGGTTTCGGTGCTGCGGTCGGTTTCTCGCTTGTATTGATCCTATTTGCTGCAATGCGTGAGCGTATTGCGGTT
GCTGATGTTCCAATGCCCTTCAAAGGCGCATCGATTGCGATGATCACAGCAGGCCTAATGTCTCTGGCATTTATGGGCTT
TACTGGGTTGGTGAAATAA

Upstream 100 bases:

>100_bases
TTTTTTTCACCAAGTGTCTAAATCCCTAGTGATCCTTGGTTGTTATTAGGTGAGCAAAGCAGGATAATATCGGGATCGGA
ATCTCAAGAATTAATCCATT

Downstream 100 bases:

>100_bases
GCATGAGCACCATCTTAATTGCAATCATTGCACTCGCCGTTTTAGCCGCCGTTTTTGGCGCTATCTTAGGCTTTGCTTCT
ATCCGCTTTAAAGTAGAAGC

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTEYLLLLVGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVSAYLVETYILTPLGIEYLRTMSFILV
IAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFAAMRERIAV
ADVPMPFKGASIAMITAGLMSLAFMGFTGLVK

Sequences:

>Translated_192_residues
MTEYLLLLVGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVSAYLVETYILTPLGIEYLRTMSFILV
IAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFAAMRERIAV
ADVPMPFKGASIAMITAGLMSLAFMGFTGLVK
>Mature_191_residues
TEYLLLLVGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVSAYLVETYILTPLGIEYLRTMSFILVI
AVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFAAMRERIAVA
DVPMPFKGASIAMITAGLMSLAFMGFTGLVK

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=192, Percent_Identity=82.2916666666667, Blast_Score=320, Evalue=3e-89,
Organism=Escherichia coli, GI1787919, Length=159, Percent_Identity=34.5911949685535, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20753; Mature: 20621

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLLLLVGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVSAYLVE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYILTPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNINENHNFIQSIIYGFGAAVGFSLVLILFAAMRERIAVADVPMPFKGASIAMITAGLM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCC
>Mature Secondary Structure 
TEYLLLLVGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVSAYLVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYILTPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNINENHNFIQSIIYGFGAAVGFSLVLILFAAMRERIAVADVPMPFKGASIAMITAGLM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLAFMGFTGLVK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA